新生牛血中Cu/Zn-SOD的提纯及肝素、壳聚糖的逐层自组装包裹

doi:10.16431/j.cnki.1671-7236.2016.08.015

Abstract

Abstract:

In order to improve the stability of super oxide dismutase (SOD) with some modification,we used thermal denaturation extraction method to extract Cu/Zn-SOD from newborn bovine blood,and used layer-by-layer self-assembly technique,heparin and chitosan as material to modificate the SOD which separated and purified from newborn bovine blood.The results showed that compared with the natural SOD,the modified SOD increased 3.3 times in the thermal stability;In acid and alkaline,under the condition of pH 3.0 and pH 11.0,the acid-base resistance property of the modified SOD raised 2.0 times;On the antitrypsin hydrolysis ability,the relative enzyme activity of modified SOD had increased 2.7 times than natural SOD.The modified SOD with shell outside made of heparin and chitosan by physical modification could get better effect,and had the strong mechanical strength,better biocompatible,outer surface of chitosan was helpful to cross the cell membrane transport,and it could be applied to the development of oral dosage forms of SOD.

Key words: SOD; heparin; chitosan; layer-by-layer self-assembly technique; relative enzyme activity

CLC Number:

S852.5

HOU Jing, LIN Chun-li, BAI Zhi-jun, WEI Jian-min, ZHANG Yong-ming, ZHANG Feng. Separation and Purification of Cu/Zn-SOD from Newborn Bovine Blood and Prepatation of HEP and CHI Using Layer-by-layer Self-assembly Technique[J]. , 2016, 43(8): 2032-2037.

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Separation and Purification of Cu/Zn-SOD from Newborn Bovine Blood and Prepatation of HEP and CHI Using Layer-by-layer Self-assembly Technique

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