1株鸽Ⅰ型副黏病毒的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2016.07.030

Abstract

Abstract:

A pigeon paramyxovirus serotypeⅠ(PPMV-1) isolate was recovered from samples collected from dead pigeons and initial isolation of the virus was performed in 9-day-old specific-pathogen-free (SPF) chicken embryos.The isolate was identified by the hemagglutination (HA) test,hemagglutination-inhibition (HI) assays,RT-PCR,sequencing and analysis of partial of F gene of the virus.The virulence and pathogenicity of the isolate were determined by mean death time (MDT),intracerebral pathogenicity index (ICPI) in 1-day-old chicks and animal regression experiment.The results indicated that one PPMV-1 was isolated from dead pigeon and named as GXP120012.The F gene sequence analysis of PPMV-1 GXP120012 strain showed that the cleavage sites of the virus was ¹¹²R-R-Q-K-R-F¹¹⁷motif in accordance with characteristic of velogenic strains;The nucleotide sequence homologies of F gene between strain GXP120012 and PPMV-1 reference strain pi/CH/LLN/110713 were high and reached 98.9%.They located the same branch on phylogenetic tree and fell into genotype Ⅵ.The MDT and ICPI values of isolate were 102 h and 0,respectively.The results of animal regression experiment showed that GXP120012 isolate was virulent for pigeons and avirulent for chickens.The present study provided useful material and theoretical basis for the study of molecular epidemiology and prevention and control of Newcastle disease in pigeon in future.

Key words: pigeon paramyxovirus serotypeⅠ; isolation and identification; pathogenicity; genotype

CLC Number:

S852.65⁺9.2

WEI Tian-chao, ZHAI Guo-sheng, LI Hai-qiong, PAN Chao, NONG Hai-lian, HUANG Wen-fei, MO Mei-lan, WEI Ping. Isolation and Identification of One Strain of Pigeon Paramyxovirus SerotypeⅠ[J]. , 2016, 43(7): 1856-1861.

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Isolation and Identification of One Strain of Pigeon Paramyxovirus SerotypeⅠ

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