C基因型牛副流感3型病毒的分离鉴定

doi:10.16431/j.cnki.1671-7236.2016.05.037

Abstract

Abstract: To identify the infection agents from Ningxia Hui Autonomous region, where feedlot cattle indicated bovine respiratory disease complex (BRDC), the M gene of the bovine parainfluenza virus type 3 was amplified by RT-PCR.The PCR product was ligated to pMD18-T vector and cloned to E.coli DH5α.The positive clones were sequenced and compared with the reference strains in GenBank by the molecular biology software.Sequence alignment results showed that a BPIV3 strain was isolated from the samples and named NX49, the M gene of NX49 included 1 056 nucleotides.Evolutionary analysis showed that the NX49 belonged to BPIV3 C genotype and shared 99.4% nucleotide identity with that of the SD0835 isolated in Shandong province.The characterization of the NX49 demonstrated that it was sensitive to temperature, acid and organic matter.The presence of Mg²⁺showed no protection against the treatment at high temperature.The HA test suggested that the NX49 enables to agglutinate the guinea pig RBC at 4 ℃ and the titer was 1∶4.The study isolated a BPIV3 genotype C strain successfully, which facilitate the study of molecular evolution and epidemiology of BPIV3 in China.

Key words: BPIV3; genotype C; isolation; identification; homologous comparison

CLC Number:

S852.65

RAN Xu-hua, ZHANG Yao, CAO Si, LU Yuan-he, ZHANG Ling-ling, LIU Yang-yang, NI Hong-bo, ZHU Zhan-bo, WEN Xiao-bo. Isolation and Identification of BPIV3 Genotype C[J]. , 2016, 43(5): 1368-1373.

References

[1] Ellis J A.Bovine parainfluenza-3 virus[J].The Veterinary clinics of North America Food Animal Practice,2010,26(3):575-593.
[2] Neill J D,Ridpath J F,Valayudhan B T.Identification and genome characterization of genotype B and genotype C bovine parainfluenza type 3 viruses isolated in the United States[J].BMC Veterinary Research,2015,11:112.
[3] 刘鹏,侯喜林,周玉龙,等.牛副流感病毒3型的分离鉴定[J].微生物学通报,2009,36(9):1384-1389.
[4] Zhu Y M,Shi H F,Gao Y R,et al.Isolation and genetic characterization of bovine parainfluenza virus type 3 from cattle in China[J].Veterinary Microbiology,2011,149(3-4):446-451.
[5] Wen Y J,Shi X C,Wang F X,et al.Phylogenetic analysis of the bovine parainfluenza virus type 3 from cattle herds revealing the existence of a genotype A strain in China[J].Virus Genes,2012,45(3):542-547.
[6] 王海勇,童钦,王炜,等.我国牛副流感病毒3型血清学调查[J].中国预防兽医学报,2014,36(2):154-156.
[7] 师新川,温永俊,王凤雪,等.牛副流感病毒3型RT-LAMP检测方法的建立及应用[J].中国畜牧兽医,2012,39(7):31-34.
[8] 薛娟,曹思,张峣,等.牛副流感病毒3型N基因的克隆与系统进化分析[J].动物医学进展,2015,36(3):32-34.
[9] Frank G H,Marshall R G.Relationship of serum and nasal secretion-neutralizing antibodies in protection of calves against parainfluenza-3 virus[J].American Journal of Veterinary Research,1971,32(11):1707-1713.
[10] Takimoto T,Portner A.Molecular mechanism of paramyxovirus budding[J].Virus Research,2004,106(2):133-145.
[11] Abinanti F R,Huebner R J.The serological relationships of strains of parainfluenza 3 virus isolated from humans and cattle with respiratory disease[J].Virology,1959,8(3):391-394.
[12] Andrewes C H,Bang F B,Chanock R M,et al.Para-influenza viruses 1,2,and 3:Suggested names for recently described myxoviruses[J].Virology,1959,8(1):129-130.

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Isolation and Identification of BPIV3 Genotype C

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