Abstract: To investigate molecular epidemiology of pseudorabies virus(PRV) in recent years in Hunan province and control pseudorabies more better, four wild-type PRV strains were detected from porcine brain tissues, which were collected from four farms of Pingjiang, Miluo, Liuyang and Changsha in Hunan province during 2012-2014.The four PRV strains were isolated by inoculating porcine brain tissues to the PK-15 cells, identified by PCR test and animal inoculation, and named PRV-XiangA, PRV-GA, PRV-YY and PRV-LY, respectively.The results showed the nucleotide homology among the four strains was 99.8% to 100.0%, by aligning the immune(gB, gG, gH, gI, gL and gM) and virulence genes(gE, PK and TK) of the four PRV strains, which indicated that these strains displayed almost no differences.The results of nucleotide alignment between the four strains and main strains isolated at home and abroad showed that the four strains had a high homology with PRV main strains isolated from China, with displaying nucleotide identity of 99.8% to 100.0% to BJ-YT and TJ strains, which revealed that the epidemic PRV strains in China had a little variation currently.

Key words: pseudorabies virus; isolation and identification; homology; immune and virulence genes; sequence analysis