梅山猪Mx1基因第14外显子多态性及其与繁殖性能的关联分析

doi:10.16431/j.cnki.1671-7236.2015.12.025

Abstract

Abstract: To analyze the effect of different genotypes in Mx1 gene exon 14 on the reproductive performance in Meishan pigs,PCR-RFLP method was used to amplify DNA and detect the polymorphisms of Mx1 gene exon 14, and the relationship between different genotypes and the reproductive performance was further analyzed by generalized linear model (GLM) in this study.The results of PCR-RFLP showed that there was a G36T mutation on Hin6 Ⅰ restriction site in Mx1 gene exon 14 and three genotypes were detected named as AA,AB and BB,in which BB was a dominant genotype.The results of correlation analysis showed that the reproductive performance of three genotypes in Mx1 gene presented the increasing tendency of BB >AB >AA in total number born (TNB),number born alive (NBA),little size at weaning (LBW),birth weight (BW) and weaning weight (WW) in gilts sows on the whole.Moreover,there was significant difference in TNB,NBA,LBW and WW in gilts sows (P< 0.05).However,there was no significant difference among all reproductive performance in multiparous sows (P >0.05).The results of this study indicated that the polymorphisms of Mx1 gene exon 14 had significantly genetic effects on production performance in Meishan gilts sows instead of multiparous sows.

Key words: Meishan pigs; Mx1 gene; polymorphisms; reproductive performance

CLC Number:

S813.3

DAI Chao-hui, DAI Kai-yu, ZONG Qiu-fang, BAO Wen-bin, WU Sheng-long. Polymorphism of Mx1 Gene Exon 14 and its Relationship with Reproductive Performance in Meishan Pigs[J]. , 2015, 42(12): 3282-3287.

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Polymorphism of Mx1 Gene Exon 14 and its Relationship with Reproductive Performance in Meishan Pigs

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