华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析

doi:10.16431/j.cnki.1671-7236.2015.11.030

Abstract

Abstract: In order to disclose the genetic variation of PRRSV,65 materials from some areas were identified during the period from 2013 to 2014.Viruses were isolated from the suspected PRRS samples.Four isolates were isolated and identified by RT-PCR and IFA.Partial Nsp2 amino acid sequences of the isolates were obtained by sequencing,and comparison analysis showed that all of the isolates were variant strains.Then we found a discontinuous deletion of 30 amino acids in Nsp2.It shared the same feature with JXA1 and TJ.Besides,we sequenced and analyzed the GP5 amino acids of 23 currently epidemic strains with some typical strains published online earlier,the results showed that all strains were American strain,21 of which had high homology with highly pathogenic variant strains (JXA1,TJ and HUN4).Moreover,further analysis of the GP5 protein suggested that these strains exhibited variations in the primary neutralizing epitope and N-linked glycosylation sites.The other two strains had the same genetic features with RespPRRSV MLV.The results showed that the highly pathogenic PRRSV was still the dominant strain and GP5 amino acid mutations were frequently,there were some genetic differences between PRRSV in different regions.

Key words: porcine reproductive and respiratory syndrome; isolation and identification; sequence analysis

CLC Number:

S852.65

LI Bin, WANG Hao-nan, PENG Zhong, HU Rui-ming, WU Bin. Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China[J]. , 2015, 42(11): 3026-3036.

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Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China

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