犬细小病毒的分离鉴定及其VP2基因序列分析

doi:10.16431/j.cnki.1671-7236.2015.10.011

Abstract

Abstract: A strain of virus was isolated and identified from feces of a suspected canine parvovirus (CPV) infected dog.It was eventually identified as CPV using electron microscope examination,hemagglutination (HA) test,animal regression experiment and molecular biology idenfication,designated as BJ-24 strain.The VP2 gene sequence analysis of BJ-24 strain showed that BJ-24 strain belonged to CPV-2a subtype.The analysis of VP2 gene indicated that the BJ-24 VP2 gene shared higher homology of nucleotide with 19 CPV strains available in GenBank and were more than 98.7%.The highest homology was 100.0% with CPV-JS2 strain.Phylogenetic tree analysis showed that BJ-24 strain belonged to China cluster and there was the closest distant between BJ-24 strain and CPV-JS2 strain.This study provided the basic evidence for further study on molecular epidemiology in Beijing and laid the solid foundation for developing vaccine and preventing the spread of CPV.

Key words: canine parvovirus; isolation; identification; sequence analysis

CLC Number:

LIN Peng, ZHAO Hang, WANG Jian-ke, CHENG Yue-ning, REN Jing-qiang, YI Li, TONG Ming-wei, CHENG Shi-peng. Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene[J]. , 2015, 42(10): 2587-2593.

References

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Isolation and Identification of Canine Parvovirus Virus and Sequence Analysis of VP2 Gene

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