噬菌体vB_EcoM-Bp7裂解酶基因的密码子使用偏性分析

doi:10.16431/j.cnki.1671-7236.2015.09.003

Abstract

Abstract: Bioinformatics softwares were used to analyze the codon bias of phage vB_EcoM-Bp7.Using Mobyle cusp of CodonW 1.4.4 and the program calculated the codon bias indicators, and calculated codon usage patterns of lysozyme gene of 7 phages in total GC content, the codon in the first and second position GC content and the codon in the third position GC content.Using MegAlign software analysis the amino acid sequence of 7 phage lyase, phylogenetic tree.The results manifested that codon usage patterns of lysozyme gene of Bp7 were basically consistent with those of other five phage lysozyme genes except phi68.Bp7 lysozyme preferred to use the A or U ending codon.Twelve kinds of high-frequency codons were identified besides in addition to AUG (Met) and UGG (Trp).Bias comparison of lysozyme codon among related phages showed that Bp7 was most similar with IME08, JS98 came second.Both phylogenetic relationship and cluster analysis demonstrated that Bp7 lysozyme was the closest with IME08 lysozyme.The results would provide further guidance for the evolution research of phage Bp7.

Key words: phage; vB_EcoM-Bp7; lysozyme gene; codon bias

CLC Number:

LU Guo-min, ZHANG Can, ZOU Ling, LIU Wen-hua, WEN Jian-xin, REN Hui-ying. Analysis of Codon Bias on Lysozyme Gene of Phage vB_EcoM-Bp7[J]. , 2015, 42(9): 2233-2239.

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Analysis of Codon Bias on Lysozyme Gene of Phage vB_EcoM-Bp7

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