毒害艾美耳球虫感染对雏鸡肠道组织热休克蛋白-90mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2015.08.037

Abstract

Abstract: The 90 chickens were randomly divided into control group, E.necatrix one time infection group and E.necatrix two times infection group.We used duplex PCR method to test the expression changes of HSP-90 mRNA in intestinal tissue of chickens infected with E.necatrix and researched the effects of it comprehensively and systematically.The results were showed that after 0 to 14 days the expression of HSP-90 mRNA in intestinal tissues of 14-day-old chickens infected with E.necatrix presented a upward trend though it was lower than the control chickens.These chickens were infected with E.necatrix again at 28 days, HSP-90 mRNA expressions of duodenum, jejunum and ileum decreased and then increased.However, it always showed a downward trend in appendix.When 28 days chickens were infected with high-does E.necatrix once, HSP-90 mRNA expressions of duodenum, jejunum and appendix were in a declining curve.While it was increased originally and then decreased in ileum.This study indicated that HSP-90 mRNA expressions in intestinal tissue of chickens infected with E.necatrix were inhibited at first, and the expression quantity was closely related to the degrees of intestinal tissue damage.

Key words: E.necatrix; chicken; intestinal tissue; HSP-90 mRNA expression

CLC Number:

Q786

HOU Ning, LV Zhi-hui, ZHAO Liang, GAO Xue-li, LIU Chao-nan, LV Xiao-ping, ZHENG Shi-min. Effect of Heat Shock Protein-90 (HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix[J]. , 2015, 42(8): 2183-2188.

References

[1] Martin W S,Adrian L S,Damer P B.Challenges in the successful control of the avian coccidia[J].Vaccine,2007,25:5540-5547.
[2] 刘红霞,张德祯.鸡球虫疫苗的研究进展[J].当代畜牧,2014,1:59.
[3] 曾彩霞.鸡球虫病的预防和治疗[J].畜牧兽医杂志,2013,32(2):116.
[4] 周容.鸡球虫病的病因及防治措施[J].中国畜牧兽医文摘,2012,28(12):129-130.
[5] Hafez H M.Poultry coccidiosis:Prevention and control approachs[J].Archiv Fur Geflugelkunde,2008,72(1):2-7.
[6] 韩玲,李培英.鸡球虫病免疫学研究进展[J].动物医学进展,2005,26(8):26-30.
[7] 钱根林,李建,樊彦红,等.鸡球虫疫苗研究进展[J].上海畜牧兽医通讯,2013,2:28-29.
[8] 刘娣勤.鸡球虫抗原的研究进展[J].国外畜牧学,2013,33(6):71-72.
[9] 吴绍强,蒋金书,刘群,等.鸡球虫疫苗研究进展[J].畜牧兽医学报,2005,36(1):1-5.
[10] 罗浩铭,孙薇,尹建元,等.热休克蛋白90抑制剂的研究进展[J].药学学报,2010,45(7):813-820.
[11] 姚元春.HSP90α、HSP90β蛋白和mRNA在人胃癌组织中的表达及临床意义[D].合肥: 安徽医科大学,2013.
[12] 孟帅,山广志,李卓荣.Hsp90抑制剂的研究进展[J].中国抗生素杂志,2011,36(4):241-248.
[13] 何彩梅,李海燕,魏大巧,等.Hsp90抑制剂的研究进展[J].生物技术通报,2010,7:64-67.
[14] Trepel J,Mollapour M,Giaccone G,et al.Targeting the dynamic HSP90 complex in cancer[J].Nature Reviews Cancer,2010,10(8):537-549.
[15] 孙秀玲,孙同虎,薄鹏飞,等.热激蛋白90在植物发育和疾病抗性中的作用[J].生命科学,2008,20:142-146.
[16] 吴丽贤,许建华,张昆仲,等. Hsp90抑制剂新生霉素诱导HL-60细胞凋亡及其机制[J].中国药理学通报,2007,23(11):1419-1424.
[17] Grammatikakis N.The role of Hsp90N,a new member of the Hsp90 family,in signal transduction and neoplastic transformation[J].Biol Chem,2002,277(10):8312-8320.
[18] 韦慧,汪慧.热休克蛋白与寄生虫感染[J].国外医学寄生虫病分册,2000,27(6):253-257.
[19] 翟杰,吕志慧,马春霞,等.毒害艾美尔球虫感染对雏鸡肠组织热休克蛋白70表达的影响[J].畜牧兽医学报,2014,45(6):1004-1010.
[20] 刁力,李成文,盛志,等.大鼠烫伤早期肠黏膜组织热休克蛋白HSP70和HSP90的表达[J].中华烧伤杂志,2000,16(5):279-282.
[21] 雷蕾,鲍恩东.急性热应激肉鸡组织中Hsp90的表达与应激损伤[J].中国农业科学,2008,41(11):3816-3821.
[22] 李德全,黄晓元,龙剑虹,等.HSP90在离体人真皮成纤维细胞热损伤中的保护作用[J].第四军医大学学报,2008,29(4):292-294.
[23] Kurahashi T,Miyake H,Hara I,et al.Expression of major heat shock proteins in prostate cancer:Correlation with clinico pathological outcomes in patients undergoing radical prostatectomy[J].J Urol,2007,177:757-761.
[24] Didelot C,Lanneau D,Brunet M,et al.Anti-cancer therapeutic approaches based on intracellular and extracellular heat shock proteins[J].Curr Med Chem,2007,14(27):2839-2847.
[25] Blagosklonny M V,Toretsky J,Bohen S,et al.Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90[J].Proec Natl Acad Sci USA,1996,93(16):8379.

Effect of Heat Shock Protein-90 (HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix

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