两株鸡传染性支气管炎病毒的分离鉴定及S1基因序列分析

doi:10.16431/j.cnki.1671-7236.2015.08.007

Abstract

Abstract: In order to investigate the epidemiology and genetic variation of avian infectious bronchitis virus (IBV) in Guangdong province, two strains of IBV were isolated by inoculation of embryo and RT-PCR detection from diseased chickens at different farms in Zhanjiang, Guangdong province in 2013.We denoted these two strains of IBV as CK/CH/GD/ZJ10/2013 and CK/CH/GD/ZJ11/2013, respectively.Analysis of the S1 gene sequences from these two isolated strains showed that the S1 gene of CK/CH/GD/ZJ10/2013 was 1 626 bp, which encoded 542 amino acids with a cleavage site sequence of NRFRR, while the S1 gene of CK/CH/GD/ZJ11/2013 was 1620 bp, encoded 540 amino acids with a cleavage site sequence of HRRRR.Phylogenetic analysis revealed that these two isolated strains were clustered into genetic groups Ⅲ and Ⅰ(QX-like type), respectively.Homology analysis demonstrated that nucleotide and deduced amino acid sequence homologies between these two isolated strains were lower, while the homologies were higher among the same genotypes, however, the homologies were lower comparing with current vaccine strains such as H120 and H52, with which the nucleotide homologies ranged from 75.7% to 76.3% and the amino acid homologies ranged from 77.1% to 77.9%.Further analysis showed that the CK/CH/GD/ZJ10/2013 strain was formed from a recombination event between CK/CH/GX/NN11-3 and GX-NN-6.Taken together, this study provided valuable insight into prevention and control of IBV infection in Guangdong province.

Key words: avian infectious bronchitis virus; isolation; identification; S1 gene; sequence analysis

CLC Number:

S852.65

SHANG Hui-qin, LI Lin, CHEN Rui-ai, LIU Hong-bo, LIANG Mei-lan, HE Dong-sheng. Isolation,Identification and Sequence Analysis of S1 Gene of Two Strains of Avian Infectious Bronchitis Viruses[J]. , 2015, 42(8): 1963-1972.

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Isolation,Identification and Sequence Analysis of S1 Gene of Two Strains of Avian Infectious Bronchitis Viruses

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