›› 2015, Vol. 42 ›› Issue (6): 1538-1546.doi: 10.16431/j.cnki.1671-7236.2015.06.032

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Study on the Expression Profiles of A-FABP Gene in 5 Goat Breeds

YANG Jia-da   

  1. College of Environmental and Life Sciences, Kaili University, Kaili 556011, China
  • Received:2015-01-28 Online:2015-06-20 Published:2015-07-23

Abstract: In order to understand the regularities of A-FABP gene expression in Guizhou native goats,and to establish the theoretical basis of molecular marker assisted selection for meat quality traits,β-actin gene was used as control,expression levels of A-FABP gene in livers,kidneys,hearts,lungs,longissimus muscle,semimembranosus muscle and subcutaneous fat of Qiandongnan small Xiang goat,Guizhou White goat,Guizhou Black goat,Qianbei Ma goat and Nanjiang Yellow goat were detected by Real-time fluorescence quantitative PCR.The results showed that A-FABP gene was expressed in all 7 tissues of 5 goat breeds,and the expression levels of A-FABP gene mRNA were all high in subcutaneous fat of 5 goat breeds;In subcutaneous fat,which was the main tissue of A-FABP gene expression in Guizhou native goats,and in longissimus muscle and semimembranosus muscle,which were directly associated with meat production property,A-FABP gene mRNA levels were all high in Qiandongnan small Xiang goat.The results suggested that the tissue distribution patterns of A-FABP gene mRNA in Guizhou native goat breeds were similar to each other.The excellent meat quality of Qiandongnan small Xiang goat might be related with the high expression levels of A-FABP gene mRNA in it's subcutaneous fat,longissimus muscle and semimembranosus muscle.

Key words: Guizhou native goat; A-FABP gene; gene expression

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