›› 2015, Vol. 42 ›› Issue (5): 1180-1186.doi: 10.16431/j.cnki.1671-7236.2015.05.022

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Expression of Proton-dependent Oligopeptide Transporters (POTs) in Calves' Tissues

GUO Jing, YANG Hong-bo, CHEN Yin-yue, LIU Hong, ZHAO Guo-qi   

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
  • Received:2014-10-29 Online:2015-05-20 Published:2015-06-02

Abstract: This test was designed to study the expression of POTs (PepT1, PepT2, PHT1 and PHT2) mRNA in calves'tissues.Tissue-specific expression of the mRNA corresponding to peptide transporter protein in four 3-month-old Chinese Holstein calves was examined by relative quantitative RT-PCR analysis.The results showed that the expression of PepT1 mRNA in the rumen was extremely significantly higher than that in the heart and muscle (P<0.01);The expression of PepT2 mRNA in the liver and kidney was extremely significantly higher than that in the heart, spleen, thymus, muscle, rumen, reticulum, omasum and abomasum (P<0.01);The expression of PHT1 mRNA in the lung and spleen was extremely significantly higher than that in the heart and muscle (P<0.01);The expression of PHT2 mRNA in the lung and thymus was significantly higher than that in the heart, liver, kidney, spleen, muscle, rumen, reticulum, omasum and abomasum (P<0.05).The results indicated that PepT1, PepT2, PHT1 and PHT2 mRNA were respectively abundantly expressed in rumen, kidney and liver, spleen and lung, lung and thymus.

Key words: PepT1; PepT2; PHT1; PHT2; calves

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