含β-内酰胺类药物有抗奶对犊牛生长发育和血液免疫指标的影响

doi:10.16431/j.cnki.1671-7236.2017.07.016

Abstract

Abstract:

This experiment was conducted to investigate the effect of feeding antibiotic-contaminated milk on growth performance and blood immune parameters of Holstein calves. Eighteen calves (3 days old) with similar body weight were selected and randomly assigned into two feeding groups, which were fed bulk milk (control group) or antibiotic-contaminated milk (experimental group) until 60 days old. The experiment period lasted for 180 d. Bulk milk and antibiotic-contaminated milk were respectively collected on 10, 20, 30, 40, 50 d, which were used to detect milk composition content, E.coli and antibiotics in milk. Body weight and body measurement were severally measured on 30, 60, 90 and 180 d, and then average daily gain (ADG) was calculated. On 7, 15, 30, 60, 90 and 180 d, serum samples were collected from Holstein calves, and used for measure of immunoserologic indexes. The results showed that β-lactam antibiotic residues were 268.65 μg/L in antibiotic-contaminated milk, and the milk protein content, somatic cell counts (SCC), and E.coli number in antibiotic-contaminated milk were all significantly higher than that in bulk milk (P<0.05). Feeding antibiotic-contaminated milk significantly decreased body weight on 30 d and ADG in 60 d, as well as body length on 90 d and heart girth on 60 and 90 d (P<0.05). Compared with control group, the calves'IgA levels on 30, 60, and 90 d, IgM levels on 7 d, IgG levels on 30 and 60 d of experimental group were all significantly increased (P<0.05). Likewise, IL-2 and TNF-α levels on 15 and 30 d of experimental group were also significantly increased (P<0.05). It was concluded that feeding antibiotic-contaminated milk could obviously decrease calves'ADG of early lactation, and to some extent enhance the levels of serum immune parameters before or after weaning.

Key words: β-lactam antibiotic-contaminated milk; holstein calves; growth and development; serum immunoserologic indexes

CLC Number:

S823

HAN Yun-sheng, QU Yong-li, WU Jian-hao, LI Wei, YUAN Xue, PAN Qi-qi, GAO Yan. Effects of β-lactam Antibiotic-contaminated Milk on Growth Performance and Blood Immune Parameters in Holstein Calves[J]. , 2017, 44(7): 2009-2015.

References

[1] Brunton L A, Duncan D, Coldham N G, et al. A survey of antimicrobial usage on dairy farms and waste milk feeding practices in England and Wales[J]. Vet Rec, 2012, 171(12):296.
[2] Donaldson S C, Straley B A, Hegde N V, et al. Molecular epidemiology of ceftiofur-resistant Escherichia coli isolates from dairy calves[J]. Appl Environ Microb, 2006, 72:3940-3948.
[3] Selim S A, Cullor J S. Number of viable bacteria and presumptive antibiotic residues in milk fed to calves on commercial dairies[J]. J Am Vet Med Assoc, 1997, 211:1029-1035.
[4] 冯仰廉, 方有生, 莫放,等. 中国奶牛饲养标准[M]. 北京:中国农业出版社, 2004.
[5] 张丽英. 饲料分析及饲料质量检测技术[M]. 北京:中国农业大学出版社, 2003.
[6] 张立, 李娜思, 冯峰,等. 超高压液相色谱-串联质谱法测定牛奶中的β-内酰胺类抗生素及其酶抑制剂[J]. 食品安全质量检测学报, 2013,4(6):1822-1826.
[7] 朱正鹏, 单安山, 薛艳林,等. 牛乳体细胞数对牛奶品质的影响[J]. 中国畜牧杂志, 2006, 42(13):47-49.
[8] Aust V, Knappstein K, Kunz H J, et al. Feeding untreated and pasteurized waste milk and bulk milk to calves:Effects on calf performance, health status and antibiotic resistance of faecal bacteria[J]. Arch Anim Nutr, 2013, 97:1091-1103.
[9] Brosh A, Aharoni Y, Levy D, et al. Effect of dietary protein concentration and source on the growth rate and on body composition of Holstein Friesian male calves[J]. J Anim Sci, 2000, 70:527-536.
[10] 魏清甜, 李平华, 汪涵,等. 粪肠球菌替代抗生素对保育仔猪生长性能、腹泻率、体液免疫指标和肠道微生物数量的影响[J]. 南京农业大学学报, 2014, 37(6):143-148.
[11] 刘显军, 丁雪, 陈静,等. 抗菌肽和抗生素对断奶仔猪生长性能的影响[J]. 中国畜牧兽医, 2012, 39(7):252-255.
[12] Donovan D C, Franklin S, Chase C C, et al. Growth and health of Holstein calves fed milk replacer supplement with antibiotics or enteroguards[J]. J Dairy Sci, 2002, 85, 947-950.
[13] Moore D A,Taylor J, Hartman M L, et al. Quality assessments of waste milk at a calf ranch[J]. J Dairy Sci, 2009, 92:3503-3509.
[14] Hill S R, Knowlton K F, Daniels K M, et al. Effects of mill replacer composition on growth, body composition, and nutrient excretion in preweaned Holstein heifers[J]. J Dairy Sci, 2008, 91:3145-3155.
[15] Daniels K M, Hill S R, Knowlton K F, et al. Effects of milk replacer composition on selected blood metabolites and hormones in preweaned Holstein heifers[J]. J Dairy Sci, 2008, 91:2628-2640.
[16] 赵会利, 高艳霞, 李建国,等. 丁酸钠对断奶犊牛生长、血液生化指标及胃肠道发育的影响[J]. 畜牧兽医学报, 2013, 44(10):1600-1608.
[17] Blecha F, Minocha H C. Suppressed lymphocyte blastogenic response and enhanced in vitro growth of infectious bovine rhinotracheitis virus in stressed feeder calves[J]. Am J Vet Res, 1983, 44(11):2145-2148.
[18] Skrinavona V, Marounek M, Simunek J, et al. Effect of virginiamycin on digestibility of nutrients, blood and immunologic paramaters, and quality of meat in veal calves[J]. J Agric Sci, 1994, 123(2):275-278.
[19] Kidd P. Th1/Th2 balance:The hypothesis, its limitation, and implication for health and disease[J]. Altern Med Rev, 2003, 8:223-246.
[20] Srivastava U S, Thakur M L, Majumdar P K, et al. Lymphoid organ mRNA translatability in rats:Effect of protein energy undernutrition in early life[J]. J Nutr, 1987, 117(2):242-246.
[21] Matsushita K, Arima N, Fujiyoshi T, et al. Interleukin-2-mediated growth of leukemic cells in lymph nodes of patients with adult T-cell leukeinia/lymphoma[J]. Leuk Res, 1996, 20(2):135-141.

Effects of β-lactam Antibiotic-contaminated Milk on Growth Performance and Blood Immune Parameters in Holstein Calves

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