Abstract:

In order to study the effects of Fagopyrum dibotryis superfine powder on the immune function and growth and development of broilers,160 one-day-old White feather broilers were chosen and randomly divided into 4 groups. The nasal drops were given with Newcastle disease live vaccine at 7 days old,and Newcastle disease-avian influenza inactivated vaccine was given by subcutaneous injection (0.3 mL/only).After 12 h,each group was fed with different feeds,the broilers in the low and high dose groups were fed diet supplemented 4.50‰,8.75‰ Fagopyrum dibotryis superfine powder,respectively,administration for 7 d. The broilers in the positive control group were fed diet added 400 g/L Astragalus polysaccharide, continuous administration for 7 d,and feed of blank control group did not add any drugs. The lymphocytes and serum were collected and then used for the separation of lymphocytes and serum at 7,21 and 35 d after the end of the administration. The proliferation of peripheral blood lymphocytes was measured by CCK-8 method. The titer of serum was evaluated by the hemagglutination inhibition test.The body weight of each group was weighed at the same time.After that,the rats were sacrificed,the spleen and bursa of Fabricius were used to calculate the immune organ index. The results showed that compared with the control group,the F/G of high dose group,low dose group and Astragalus polysaccharide group were decreased by different degrees at 7,21 and 35 d after the end of the administration (P>0.05). In addition,the proliferation ability of the peripheral blood lymphocytes,immune antibody level and the body weight in broilers of administration groups were increased,in which the titers of AIV and NDV antibodies were extremely significantly increased in high dose group compared with the control group (P<0.01).Meanwhile the Fagopyrum dibotryis superfine powder also could increase the spleen and bursa of Fabricius indexes. In conclusion,the Fagopyrum dibotryis superfine powder could significantly improve the cellular immunity, humoral immunity and nonspecific immune function of broilers,and significantly increased the body weight. According to the specification of the results of the instructions, we could determine that it had the effect to comprehensively improve the immunity and growth of White feather broilers. The study could lay the foundation of the future development of Fagopyrum dibotryis superfine powder into a new natural herbal medicine and feed additives.

Key words: Fagopyrum dibotryis superfine powder; broilers; immune function; growth and development