从江香猪基因组甲基化修饰与体重之间的相关性研究

doi:10.16431/j.cnki.1671-7236.2015.04.030

Abstract

Abstract: To explore the epigenetics mechanism for the slow growth in Xiang pig, 60 piglets in 20 days were divided into two groups with high or low body weight. The methylation modification at cytosine in the genomic DNA from blood and liver was investigated by methylation-sensitive amplifying polymorphism (MSAP) technique. A total of 5 977 sites were counted based on MSAP results. Compared to the low-weight group, the total DNA methylation level in high-weight group was lower. Although the half DNA methylation levels in blood samples were higher than that in liver samples, the both/total DNA methylation levels in blood were lower than that in liver. It showed that the total DNA methylation level tended to decrease as the increasing of body weight. Five specific fragments from A1 to A5 were sequenced which were differently methylated between two groups. Both of A1 and A4 detected from high-weight group while the others got from low-weight group. Fragment A1 and A2 detected from blood genomic DNA and A3 to A5 from liver samples. A3 was apart far from two genes without coding region for RNA. The others were located in genes related with metabolism and immune. The results indicated that genomic DNA methylation level was different among individual piglet and related with the body weight of Xiang piglet in 20 days. The four specific methylation fragments would be useful for research on the mechanism of growth in Xiang pig breed.

Key words: Congjiang Xiang pig; genomic methylation; specific fragments; body weight

CLC Number:

S813.3

LIN Ru-tao, RAN Xue-qin, WANG Jia-fu. Relationship between Genomic DNA Methylation and Body Weight of Congjiang Xiang Pig[J]. , 2015, 42(4): 973-979.

References

[1] Jeltsch A.Beyond Watson and Crick:DNA methylation and molecular enzymology of DNA methyltransferases[J].Chembiochchem,2002,3(4):274-293.
[2] Johnson A A,Akman K,Calimport S R,et al.The role of DNA methylation in aging,rejuvenation,and age-related disease[J].Rejuvenation Res,2012,15(5):483-494.
[3] Huang L H,Wang R,Gama-Sosa M A,et al.A protein from human placental nuclei binds preferentially to 5 methyleytosine rich DNA[J].Nature,1984,308(5956):293-295.
[4] 索效军,张年.DNA甲基化、组蛋白修饰与基因沉默[J].中国畜牧兽医,2007,34(2):82-85.
[5] 邹丹丹,王晓利,汪海林.DNA甲基化结合蛋白[J].环境化学,2014,33(10):1672-1680.
[6] Ohki Ⅰ,Shimotake N,Fujita N,et al.Solution structure of the methyl CpG binding domain of human MBD1 in complex with methylated DNA[J]. Cell,2001,105(4):487-497.
[7] Xu M L,Li X Q,Korban S S.AFLP-based detection of DNA methylation[J].Plant Mol Biol Rep,2000,18(4):361-368.
[8] 杨廷模,刘畅,赵恒鑫,等.高产从江香猪资源调查及其FSH基因型分析[J].畜牧与兽医,2014,46(8):5-8.
[9] 申学林,杨秀江.从江香猪生长发育繁殖性能测定[J].种业研究,2007,11:39-41.
[10] Chen X Q,Ma Y,Chen F,et al.Analysis of DNA methylation patterns of PLBs derived from Cymbidium hybridium based on MSAP[J].Plant Cell Tiss Organ Cult,2009,98(3):67-77.
[11] Stuber C W,Lineoln S E,Wblff D W,et al.Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers[J]. Genetics,1992,132(3):823-839.
[12] 蒋曹德,邓昌彦,熊远著.DNA甲基化差异对猪生长性状的影响[J].畜牧兽医学报,2005,36(2):105-110.
[13] 李颖,唐世倩,翟瑞芳,等.S100A4及其甲基化与早期宫颈癌淋巴转移的相关性研究[J].山西医科大学学报,2013,8:641-644.
[14] Kuang Z,Lewis R S,Curtis J M,et al.The SPRY domain-containing SOCS box protein SPSB2 targets iNOs for proteasomal degradation[J].J Cell Biol,2009,190(1):129-141.
[15] Radominka-Pandya A,Czemik P J,Little J M,et al.Structural and functional studies of UDP- glucuronosylferase[J].Drug Metab Rev,1999,31:817-822.
[16] Achour B,Rostami-Hodjegan A,Barber J.Protein expression of various hepatic uridine 5'-diphosphate glucuronosyltransferase (UGT) enzymes and their inter-correlations:A meta-analysis[J].Biopharmaceutics & Drug Disposition,2014,35:353-361.
[17] 彭金咏,栾连军.尿苷二磷酸葡萄糖醛酸转移酶的研究进展[J].中国现代应用药学,2002,19(5):373-376.
[18] de Moor C H,Richter J D.Cytoplasmic polyadenylation mediate masking and unmasking of cycling B1 mRNA[J].EMBO J,2006,18(8):2294-2303.
[19] Kang M K,Han S J.Post-transcriptional and post-translational regulation during mouse oocyte maturation[J].BMB Rep,2011,44(3):147-157.
[20] Campbell Z T,Menichelli E,Friend K,et al.Identification of a conserved interface between PUF and CPEB proteins[J].The Journal of Biological Chemistry,2012,287(22):18854-18862.
[21] Voss M D,Czechtizky W,Li Z,et al.Discovery and pharmacological characterization of a novel small molecule inhibitor of phosphatidylinositol-5-phosphate 4-kinase,type Ⅱ,beta[J].Biochemical and Biophysical Research Communications,2014,449(3):327-331.
[22] 陈兴娟,张熙东,张璇.蛋白激酶C和磷脂酰肌醇4,5二磷酸之间相互调节作用的研究进展[J].中国药理学通报,2012,11:1497-1499.

Relationship between Genomic DNA Methylation and Body Weight of Congjiang Xiang Pig

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[2]	XU Pan, ZHONG De, MA Zheng, LIU Linyu, WANG Yihui, ZHAO Xuting. Identification of Candidate Genes for Body Weight in Sujiang Pigs Based on Genome-wide Differential Methylation Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2889-2900.
[3]	XU Lei, ZHANG Menghua, WANG Dan, ZHANG Xiaoxue, FAN Shoumin, LIU Jianming, LIU Jiangwei, YAN Chengxu, HUANG Xixia, WANG Yachun. Estimation of Genetic Parameters of Body Size and Body Weight in Xinjiang Brown Cattle at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4105-4114.
[4]	SI Jinglei, HUANG Ye, CHEN Kuirong, LYU Dongling, CHENG Feng, TIAN Weilong, LIU Xiaoxiao, LIANG Liang, LI Yueyue, FENG Lingli, GAO Jiuyu, MO Jiayuan, LIANG Jing, LAN Ganqiu. Evaluating of the Association Between Early-stage Weight and Gut Microbiota in Duroc Pigs Using 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 190-199.
[5]	LIU Xiangping, ZHU Jing, BAI Hao, KONG Linglin, GU Huabing, DOU Xinhong. Correlation Between Growth Traits and Immune Indexes of Xueshan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1481-1488.
[6]	CAI Chunbo, XU Kai, LI Meng, YANG Yang, ZHAO Yan, GAO Pengfei, GUO Xiaohong, CAO Guoqing, LI Bugao. Correlation Analysis Between Haplotypes of ApoA5 Gene on Body Weight and Meat Quality in Jinfen White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3563-3572.
[7]	WANG Xinyue, CAO Hang, LYU Shipeng, WANG Qiong, LIU Lingling, JUEKEN Aniwash, LIU Wujun. Morphological Variation Analysis of Fat Tail of the Backcrossing Second Generation of Wild Argali Sheep×Bashbay Sheep [J]. , 2019, 46(8): 2415-2422.
[8]	WANG Weiyong, GONG Ting, WANG Tingting, XU Yongjian, MENG Lijie. Morphological Analysis of Spermatogenic Epithelial Cycle and Testicular Development in Congjiang Xiang Pigs [J]. , 2019, 46(7): 2012-2020.
[9]	LI Honglin, LI Wei, RAO Yongchao, MU Tenghui, YANG Defeng, JIAN Huafeng, LIN Jiadong, ZHANG Fuping. Study on Growth Curve Fitting of Slow-feathering Line of Guizhou Yellow Chickens [J]. , 2019, 46(7): 2053-2058.
[10]	LU Huining, LIU Yanyan, ZHANG Li, JIN Xin, HE Linbo, CAO Xin. Correlation Analyses Between Body Weight and Body Sizes of F1 Hybrid of Lanzhou Fat-tailed Sheep×Small-tailed Han Sheep [J]. , 2019, 46(6): 1628-1635.
[11]	SHI Huibin, WANG Yuqin, HE Wengtan, WU Zhibo, TIAN Zhilong, YU Zuhua, DING Ke, ZHAO Zhanqin. The Determination of Growth Performance,Wool Quality and Blood Physiological and Biochemical Indexes of SDH Hybrid Sheep [J]. , 2019, 46(2): 404-413.
[12]	LIANG Xiaopeng, ZHANG Zhenliang, AMANKAIDI·Mohamedikhan, YAO Lidan, BAOAODUNGERILE,JUEKEN·Aniwashi. Study on Growth and Development Regularity and Its Correlation with Body Size of Xinjiang Ymule White Lamb [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2937-2945.
[13]	WEN Guilan, ZHANG Shengbo, LI Changhong, XU Li, TIAN Lang, WEN Ming, WANG Kaigong, CHENG Zhentao, ZHAO Degang. Sequence Analysis and Prokaryotic Expression of IFN-β Gene in Congjiang Xiang Pig [J]. , 2019, 46(1): 10-19.
[14]	XU Min, XU Houqiang, CHEN Wei, YANG Yang. Study on Expression of the Relate Genes Involved in Intramuscular Preadipocyte Differentiation in Congjiang Xiang Pig [J]. , 2018, 45(9): 2492-2499.
[15]	WEN Guilan, CHEN Shaopin, ZHANG Shengbo, LIN Hanqing, GUAN Guodan, WANG Kaigong, WEN Ming, ZHOU Bijun, CHENG Zhentao, ZHAO Degang. Cloning and Sequence Analysis of Interferon Gamma Gene in Congjiang Xiang Pig [J]. , 2018, 45(6): 1437-1446.