从江香猪γ干扰素基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2018.06.002

Abstract

Abstract:

This experiment was aimed to study the cloning and sequence analysis of interferon gamma (IFN-γ) gene in Congjiang Xiang pig.The total RNA of liver tissue in Congjiang Xiang pig was extracted and reversely transcribed into cDNA.The encoding region of Congjiang Xiang pig IFN-γ gene (CJ-poIFN-γ) was amplified using specific primers by nested PCR,ligated to pUCm-T vector,and verified by sequencing.Sequence analysis of CJ-poIFN-γ by NCBI,SOPMA,SignalP-4.1,DNAStar and so on.The results showed that the coding region of CJ-poIFN-γ gene was 501 bp,encoding 166 amino acids.The results of nucleotide sequence alignment showed that the homology of CJ-poIFN-γ among Congjiang Xiang pig, Meishan pig,Jianbai pig,Tibetan pig,Chenghua pig,Rongchang pig,Indian pig,Landrace pig and Neijiang pig was 99.4% to 100.0%.Phylogenetic tree analysis showed that CJ-poIFN-γ was closely related to Meishan pig,Jianbai pig,Tibetan pig and Chenghua pig;CJ-poIFN-γ gene coding protein was secretory protein,there was no transmembrane structure,and the first 23 amino acids were signal peptide sequences.The secondary structure of CJ-poIFN-γ gene coding protein was mainly α-helix (50.60%) and random coil (33.14%).The B cell epitopes were mainly located in 62-65,84-87,113-115,144-156 and 162-166 amino acids.The results laid a foundation for further study on biological activity of IFN-γ and speeding up the effective utilization of the resources in Congjiang Xiang pig.

Key words: Congjiang Xiang pig; IFN-γ gene; cloning; sequence analysis

CLC Number:

WEN Guilan, CHEN Shaopin, ZHANG Shengbo, LIN Hanqing, GUAN Guodan, WANG Kaigong, WEN Ming, ZHOU Bijun, CHENG Zhentao, ZHAO Degang. Cloning and Sequence Analysis of Interferon Gamma Gene in Congjiang Xiang Pig[J]. , 2018, 45(6): 1437-1446.

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Cloning and Sequence Analysis of Interferon Gamma Gene in Congjiang Xiang Pig

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