›› 2019, Vol. 46 ›› Issue (7): 2012-2020.doi: 10.16431/j.cnki.1671-7236.2019.07.016

• Genetics and Breeding • Previous Articles     Next Articles

Morphological Analysis of Spermatogenic Epithelial Cycle and Testicular Development in Congjiang Xiang Pigs

WANG Weiyong1,2, GONG Ting1,2, WANG Tingting2, XU Yongjian1,2, MENG Lijie1,2   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China;
    2. College of Animal Science, Guizhou University, Guiyang 550025, China
  • Received:2018-11-22 Online:2019-07-20 Published:2019-07-22

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Abstract:

This study was aimed to investigate the difference of spermatogenic epithelial cycle and the morphological changes during testicular development before and after puberty.The testicular correlation indexes at 15,30,60 and 90 d were measured,and the morphology of testicular tissue was observed to judge the puberty,and divided the spermatogenic epithelial cycle in Congjiang Xiang pigs.The results showed that the testicular index at 30 d was extremely significantly higher than that of 15 d (P<0.01).The growth rates of testicular weight,long axis and short axis were 298.05%,66.42% and 65.45%,respectively,the testicular weight growth rate was relatively stable at the two stages of 60 and 90 d.Morphological observation showed that the dissociative sperm appeared in the seminiferous tubules at 30 d of Congjiang Xiang pigs,completing the first spermatogenesis and entering the puberty phase.The seminiferous tubules area and seminiferous epithelium thickness at 30 d were extremely significantly increased relative to 15 d (P<0.01),the growth rate were 136.12% and 40.19%,respectively,it was in a stable growth state at 60 and 90 d.Statistical analysis result of the testicular cells showed that the number of setoli cells (SC) was not affected by the increase of age (P>0.05),but the number of germ cells (GC) at 30 d extremely significantly increased compared with that of 15 d (P<0.01).Correlation analysis result showed that there was a significant positive correlation between the increase of germ cell number and the increase of the seminiferous tubule area and spermatogenic epithelium thickness (r=0.994;0.96).The spermatogenic epithelium was divided into 3 and 8 stages before and after the puberty stage by the difference in the combination of germ cells.Germ cells in the first meiotic period were mainly at the first stage of meiosis,there were A and B types spermatogonia,SC,primary spermatocyte (Ps),preleptotene (PI) and leptotene (L) in the spermatogenic epithelium before and after puberty,but the round spermatids (R),elongating spermatid (E) and spermatozoa (S) were only found in the spermatogenic epithelium after puberty.This study found that Congjiang Xiang pigs reached the puberty when it was 30 days old,the testicular development was dominated by the rapid increase in the area of germ cells and seminiferous tubules.This results had important guiding significance for excavating early-maturing traits,breeding pigs,development and utilization in Congjiang Xiang pigs.

Key words: Congjiang Xiang pigs; testicles; puberty; spermatogenic epithelial cycle

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