共表达K88/987p菌毛产肠毒素性大肠杆菌的分离鉴定及其卵黄抗体的制备

doi:10.16431/j.cnki.1671-7236.2015.02.010

Abstract

Abstract: For preparation of specific IgY against the ETEC strains from the pig farms,types of fimbriae gene of an ETEC strain isolated from these pig farms were identified by PCR.The pilin was purified and used to immunize hens to obtain the IgY.The titer,specificity and in vitro inhibitory effect of IgY were detected.The results showed that this ETEC strain had two types of fimbriae gene of K88 and 987p,and the immunogenic of the strain fimbriae was high.The titers of the isolates fimbriae IgY against the K88 and 987p fimbriae were both 1:64 000.The IgY could bind with K88 and 987p but could not either with K99 or F41. 5 mg/mL fimbriae IgY could completely inhibit the growth of K88 and 987p in vitro.

Key words: K88; 987p; co-expression; pilin; IgY; in vitro inhibition

CLC Number:

S852.5

PENG Jiao, LI Zhi, QI Zhen-qiang, LU Chao. Isolation of the ETEC Strain Co-expressed K88/987p Fimbriae and Preparation of its Yolk Antibodies[J]. , 2015, 42(2): 303-308.

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Isolation of the ETEC Strain Co-expressed K88/987p Fimbriae and Preparation of its Yolk Antibodies

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