N-羟乙酰神经氨酸IgY抗体的制备及鉴定

doi:10.16431/j.cnki.1671-7236.2016.03.010

Abstract

Abstract: The experiment was carried out to establish a method for preparation and identification of N-glycolylneuramic acid IgY antibody.Neu5Gc was linked to carrier proteins by carbodiimide(EDC)method.After the immunization of immunogen to laying hens,the IgY antibody was gained.Neu5Gc-IgY was analyzed and identified by ELISA.The results of UV and agarose gel electrophoresis showed that the artificial antigens of Neu5Gc were successfully synthesized.The development of the IgY antibody titer was monitored by indirect ELISA.The result showed that the IgY antibody was generated at 7th day after the first immunization.The titer was gradually increased and reached its peak (1:10 000) at 63th day after the first immunization.The high titer of the IgY antibody maintained through the whole observation period.The sensitivity of anti-Neu5AGc IgY antibody was detected by the competitive blocking ELISA.The linearity about the first chicken of the standard curve showed good,the liner regression equation was y=30.28x+16.923,R²=0.9581.The IgY antibody gained in this study laid the foundation for the establishment of indirect ELISA immunoassay method for detecting Neu5Gc in red meat,milk and tumor tissues.

Key words: Neu5Gc; artificial antigen; laying hen; IgY

CLC Number:

S852.4⁺3

WU Zong-cheng, GAO Yang, HU Pan, LI Yan-song, ZHOU Yu, LIU Zeng-shan, REN Hong-lin, LU Shi-ying. Preparation and Identification of N-glycolylneuraminic Acid IgY Antibody[J]. , 2016, 43(3): 629-636.

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Preparation and Identification of N-glycolylneuraminic Acid IgY Antibody

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