利巴韦林对猫细小病毒感染诱导F81猫肾细胞中凋亡相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2015.02.011

Abstract

Abstract: The F81 cells were divided into three groups,control group,feline panleukopenia virus (FPV) infected group and ribavirin treatment group,we used Real-time fluorescent quanlitative PCR to detect the expression of Bcl-2,Bcl-xl,Bax and Caspase 3.Real-time fluorescent quanlitative PCR results showed that the number of apoptotic cells increased in FPV infected group compared with the control group,and mRNA levels of Bcl-2 was inhibited,which was an anti-apoptotic gene,while the expressions of Bax and Caspase 3 were both increased.In ribavirin treatment group,expression levels of Bcl-2 and Bcl-xl were significantly higher than that of FPV infected group (P<0.05),and had a peak at 48 h in all time points.However,mRNA levels of Bax and Caspase 3 in ribavirin treatment group were significantly lower than that of FPV infected group (P<0.05),and the lowest levels were at 72 h in all time points.In a word,ribavirin could inhibit the apoptosis of F81 cells infected by FPV at the genetic level.

Key words: ribavirin; F81 cells; gene expression; cell apoptosis

CLC Number:

Q786

HU Lian-mei, ZHENG Liang-yan, YU Wen-lan, LI Ya-feng, TANG Zhao-xin. Effects of Ribavirin on Expression of Apoptotic Related Genes of F81 Cells Infected by Feline Panleukopenia Virus[J]. , 2015, 42(2): 309-316.

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Effects of Ribavirin on Expression of Apoptotic Related Genes of F81 Cells Infected by Feline Panleukopenia Virus

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