瘤胃源益生菌对小鼠生长和生理特性的影响

doi:10.16431/j.cnki.1671-7236.2018.09.015

摘要/Abstract

摘要：

试验旨在对筛选出的6种瘤胃源益生菌进行小鼠试验，以期为其在畜禽养殖业的进一步开发和利用提供参考依据。选取5周龄雄性昆明小鼠56只，随机分为7组：生理盐水对照组、解淀粉芽孢杆菌Lw-J21组、枯草芽孢杆菌Lw-K16组、乳酸菌Lw-R35组、产蛋白酶菌Lw-D48组、产纤维素酶菌Lw-X21组和酵母菌Lw-JM70组，试验组每天以0.2 mL/10 g体重剂量连续灌胃42 d，对照组灌喂相同剂量的生理盐水，比较并分析灌胃不同益生菌对小鼠体重、肠道菌群数量、脂肪积累量、免疫器官指数和血糖降解能力等的影响。结果显示，与对照组相比，解淀粉芽孢杆菌Lw-J21和产纤维素酶菌Lw-X21组小鼠体重增加明显（P>0.05），同时可显著减少小鼠体内的脂肪积累（P<0.05）。乳酸菌Lw-R35和产纤维素酶菌Lw-X21组小鼠肠道中的乳酸菌和总厌氧菌数量均显著或极显著增多（P<0.05；P<0.01）。各益生菌组小鼠脾脏指数和胸腺指数均有显著或极显著提高（P<0.05；P<0.01），且可不同程度提高小鼠血糖的调节能力。综上所述，用筛选出的6种天然益生菌饲喂小鼠可改善其肠道微生态环境、免疫器官指数和血糖调节能力等。

关键词: 益生菌; 肠道菌群; 脂肪积累量; 免疫器官指数; 血糖

Abstract:

The experiments of six kinds of probiotics screened from the rumen were conducted to provide references for their further development and utilization in livestock and poultry husbandry.56 male 5-week-old Kunming mice were chosen and randomly divided into 7 groups.The mice in control group was fed with 0.2 mL of normal saline,while that of the other experimental groups were fed with 0.2 mL/(10 g·BW) of Bacillus amyloliquefaciens Lw-J21,Bacillus subtilis Lw-K16,Lactobacillus Lw-R35,Bifidobacterium Lw-D48,cellulose-producing bacteria Lw-X21,Candida dattila Lw-JM70 (1×10⁷ CFU/mL) for 42 d,respectively.The effects of different probiotics on body weight,intestinal flora,fat accumulation,immune indexes and hypoglycemia of mice were compared and analyzed.The results showed that compared with the control group,the body weight of mice in Lw-J21 and Lw-X21 groups were higher (P>0.05), and the fat accumulation in mice were significantly reduced at the same time (P<0.05).The number of Lactobacillus and total anaerobic bacteria in the intestinal tract of mice in Lw-R35 and Lw-X21 groups were significantly or extremely significantly increased (P<0.05;P<0.01).The spleen index and thymus index of mice in probiotics groups significantly or extremely significantly enhanced (P<0.05;P<0.01),meanwhile the hypoglycemia of mice was improved.In conclusion,the screed probiotics had different degrees of influence on intestinal microenvironment, immune indexes and blood glucose regulation ability of mice.

Key words: probiotic; intestinal flora; fat accumulation; immune organ indexes; blood glucose

中图分类号:

S816.32

冀海龙, 王红英, 周英男, 钱斯日古楞. 瘤胃源益生菌对小鼠生长和生理特性的影响[J]. 《中国畜牧兽医》, 2018, 45(9): 2463-2470.

JI Hailong, WANG Hongying, ZHOU Yingnan, QIAN Siriguleng. Effects of Rumen-derived Probiotics on the Growth and Physiological Characteristics of Mice[J]. , 2018, 45(9): 2463-2470.

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