竹酢粉对妊娠后期母羊血液及初乳抗氧化、免疫指标的影响

doi:10.16431/j.cnki.1671-7236.2018.01.014

摘要/Abstract

摘要：

本试验旨在研究竹酢粉对妊娠后期母羊血液及其初乳抗氧化和免疫指标的影响。试验选择胎次一致、身体状况良好的妊娠后期（妊娠约120 d）湖羊10只，随机分成2组，分别饲喂基础日粮（对照组）和基础日粮+2%竹酢粉（试验组），试验期3周（1周预饲期+2周正式试验期），试验结束采集血液测定生化、免疫和抗氧化指标；分娩1周内采集初乳测定免疫和抗氧化指标。结果显示：①试验组母羊血液总蛋白（TP）含量和谷丙转氨酶（GPT）活性显著高于对照组（P<0.05），谷草转氨酶/谷丙转氨酶（GOT/GPT）和血小板数量则显著低于对照组（P<0.05），而红细胞数量、血红蛋白浓度均高于对照组母羊，但差异并不显著（P>0.05）；②与对照组相比，试验组母羊血清的免疫球蛋白A（IgA）、白细胞介素-6（IL-6）含量显著升高（P<0.05），总抗氧化力（T-AOC）、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）含量极显著升高（P<0.01），肿瘤坏死因子-α（TNF-α）含量低于对照组但无显著差异（P>0.05）；③竹酢粉能够显著降低母羊初乳TNF-α的含量（P<0.05），显著升高初乳超氧化物歧化酶（SOD）活性（P<0.05），但对初乳免疫球蛋含量无显著影响（P>0.05）。本研究结果表明，日粮中添加竹酢粉能改善妊娠后期湖羊的健康状况，提高母羊的抗氧化和免疫性能。

关键词: 竹酢粉; 抗氧化; 免疫性能; 妊娠后期母羊

Abstract:

To study the effects of bamboo vinegar powder on the antioxidant and immune indexes of blood and colostrums in late-pregnant Hu ewe, ten healthy late-pregnant primiparous Hu ewes at about 120 days were chosen and randomly divided into 2 groups with 5 ewes per group. The basic diet and the basic diet added with 2% of bamboo vinegar powder were fed to the ewes in control and experimental groups respectively, and the trial period were 3 weeks including 1 week of preparatory feeding and 2 weeks of formal trial period. The blood samples were collected and detected for the biochemical, immune and antioxidant indexes after finishing experiment. The colostrum was collected within a week after parturition and measured for immune and antioxidant indexes also. The results showed that:①Compared with the ewes in control group, the total protein (TP) content and glutamic-pyruvic transaminase (GPT) activity of blood in experimental ewes were significantly increased (P<0.05), while the glutamic oxalacetic transaminase (GOT)/GPT and platelet counts were significantly decreased (P<0.05), and the red blood cell counts and the hemoglobin concentration were increased with no significant difference (P>0.05);②Compared with the control group, the serum interleukin-6(IL-6) and immune globulin A(IgA) contents of ewes in experimental group were increased significantly (P<0.05), the total antioxidant capacity (T-AOC), immune globulin G(IgG) and immune globulin M(IgM) contents were extremely significantly increased (P<0.01), the tumour necrosis factor-α(TNF-α) content was decreased with no significant difference (P>0.05);③Bamboo vinegar powder significantly decreased the TNF-α content (P<0.05), increased the superoxide dismutase(SOD) activity (P<0.05) of colostrum, but the immunoglobulin content had no significant changes(P>0.05). In conclusion, diet supplemented with bamboo vinegar powder could improve the healthy and enhance the antioxidant and immune performance of late-pregnant Hu ewe.

Key words: bamboo vinegar powder; antioxidant; immune performance; late-pregnant ewe

中图分类号:

S816.7

刘明美, 徐长军, 黄德军, 王伟伟, 占今舜, 霍永久, 赵国琦. 竹酢粉对妊娠后期母羊血液及初乳抗氧化、免疫指标的影响[J]. 《中国畜牧兽医》, 2018, 45(1): 106-112.

LIU Mingmei, XU Changjun, HUANG Dejun, WANG Weiwei, ZHANG Jinshun, HUO Yongjiu, ZHAO Guoqi. Effects of Bamboo Vinegar Powder on the Antioxidant and Immune Indexes of Blood and Colostrums in Late-pregnant Ewe[J]. , 2018, 45(1): 106-112.

参考文献

[1] 朱世平,霍永久,喻礼怀,等.竹酢液及其在养猪生产中的应用[J].养猪,2014,2:22-24. ZHU S P, HUO Y J, YU L H,et al.Bamboo vinegar and its application in swine[J].Swine Production,2014,2:22-24. (in Chinese)
[2] CHU G M, JUNG C K, KIM H Y, et al. Effects of bamboo charcoal and bamboo vinegar as antibiotic alternatives on growth performance, immune responses and fecal microflora population in fattening pigs[J]. Animal Science Journal, 2013, 84(2):113-120.
[3] HUO Y J, LIU Z X, XUAN H, et al. Effects of bamboo vinegar powder on growth performance and mRNA expression levels of interleukin-10,interleukin-22,and interleukin-25 in immune organs of weaned piglets[J].Animal Nutrition,2016,2(2):111-118.
[4] 董丽,仲召鑫,王淑楠,等.竹酢粉对育肥猪肉品质和风味的影响[J].动物营养学报,2017,29(3):916-922. DONG L,ZHONG Z X,WANG S N,et al. Effects of bamboo vinegar powder on meat quality and meat flavor of finishing pigs[J].Chinese Journal of Animal Nutrition,2017,29(3):916-922.(in Chinese)
[5] 蒋竹英,范觉鑫,陈明洪,等.脱氧雪腐镰刀菌烯醇污染饲粮对断奶仔猪血液生理生化指标的影响及竹炭和竹醋液的干预作用[J].动物营养学报,2012,24(12):2459-2468. JIANG Z Y,FAN J X, CHEN M H,et al. Effects of diets contaminated by deoxynivalenol on blood physiological and biochemical indexes and intervention effects of bamboo-carbon and bamboo vinegar in weaner piglets[J].Chinese Journal of Animal Nutrition,2012,24(12):2459-2468. (in Chinese)
[6] 蒋竹英,李丽立,唐利华,等.DON污染饲粮添加竹炭和竹醋液对断奶仔猪抗氧化性能及小肠黏膜形态的影响[J]西北农林科技大学学报(自然科学版),2013,41(12):37-42. JIANG Z Y, LI L L, TANF L H,et al.Effects of adding bamboo charcoal and bamboo vinegar to DON contaminated diets on oxidation resistance and small intestinal morphology of weaned piglets[J].Journal of Northwest A & F University(Natural Science Edition),2013,41(12):37-42. (in Chinese)
[7] 蒋竹英,李铁军,唐利华,等.竹炭和竹醋液对断奶仔猪生长性能、血液生理生化指标和抗氧化性能的影响[J].华中农业大学学报,2013,32(3):88-93. JIANG Z Y,LI T J,TANF L H,et al. Effects of bamboo-carbon and bamboo vinegar on blood indexes and antioxidant capacity in weaned piglets[J].Journal of Huazhong Agricultural University, 2013,32(3):88-93. (in Chinese)
[8] WANG H F, GAO K, WANG C, et al. Effects of feeding bamboo vinegar and acidifier as an antibiotic substitute on the growth performance and intestinal bacterial communities of weaned piglets[J]. Acta Agriculturae Scandinavica Section A-Animal Science, 2013, 63(3):143-150.
[9] YAN L, KIM I H, HUH K. Influence of bamboo vinegar supplementation on growth performance, apparent total tract digestibility, blood characteristics, meat quality, fecal noxious gas content, and fecal microbial concentration in finishing pigs[J]. Livestock Science, 2012, 144:240-246.
[10] CHEN L H, CHAI Y L, SHUK M K, et al. Bamboo vinegar decreases inflammatory mediator expression and NLRP3 inflammasome activation by inhibiting reactive oxygen species generation and protein kinase c-α/σ activation[J]. PLoS One, 2013, 8(10):e75738.
[11] 郭峰.红细胞天然免疫与获得性免疫[J].自然杂志,2004,26(4):194-199. GUO F. Natural immune of erythrocytes and acquired immunity[J].Chinese Journal of Nature,2004,26(4):194-199. (in Chinese)
[12] 刘玥,殷惠军,史大卓,等.活血化瘀中药与抗血小板治疗[J].科学通报,2014,59(8):647-655. LIU Y, YIN H J, SHI D Z, et al. The use of Chinese medicinal herbs and formulas that activate blood circulation and antiplatelet therapies[J].Chinese Science Bulletin,2014,59(8):647-655. (in Chinese)
[13] 周玉香,吕玉玲,王洁,等.血液生化指标在动物生产与营养调控研究中的应用概况[J].畜牧与饲料科学,2012,33(5-6):72-73. ZHOU Y X,LV Y L,WANG J, et al. Application survey of blood biochemical indices in the studies of animal production and nutritional regulation[J].Animal Husbandry and Feed Science,2012,33(5-6):72-73.(in Chinese)
[14] WANG Q, GE X, TIAN X, et al. Soy isoflavone:The multipurpose phytochemical (Review)[J]. Biomedical Reports, 2013, 1(5):697-701.
[15] 翟钦辉,荣岳光,董晓芳,等.苜草素对巨噬细胞吞噬活性和NO、IL-6、TNF-α分泌的影响[J].营养学报,2012,34(2):181-185. ZHAI Q H, RONG Y G, DONG X F, et al. Effects of polysavone on phagocytic activity and NO, IL-6, and TNF-α production of macrophages[J].Acta Nutrimenta Sinica,2012,34(2):181-185. (in Chinese)
[16] 丘创华,侯敢,黄迪南.TNF-α信号传导通路的分子机理[J].中国生物化学与分子生物学报,2007,23(6):430-435. QIU C H, HOU G, HUANG D N. Molecular mechanism of TNF-α signal transduction[J].Chinese Journal of Biochemistry and Molecular Biology,2007,23(6):430-435. (in Chinese)
[17] 刘正旭.竹酢粉对断奶仔猪生产性能、免疫机能及肠道微生物区系的影响[D].扬州:扬州大学,2016. LIU Z X. Effects of bamboo vinegar powder on production performance, immune function and intestinal microbiota of weaned pigs[D].Yangzhou:Yangzhou University,2016.(in Chinese)
[18] 蒋竹英.脱氧雪腐镰刀菌烯醇污染饲粮添加竹炭和竹醋液对断奶仔猪的影响研究[D].长沙:湖南农业大学,2013. JIANG Z Y.Effects of adding bamboo charcoal and bamboo vinegar to DON contaminated diets on weaned pigs[D].Changsha:Hunan Agricultural University,2013.(in Chinese)
[19] 钱华,王衍彬,张邦川,等.竹醋液及其提取物清除DPPH·自由基活性的研究[J].浙江林业科技,2007,27(6):24-27. QIAN H, WANG Y B,ZHANG B C,et al. Antioxidant activity test on bamboo vinegar and its extract by DPPH·method[J].Journal of Zhejiang Forestry Science and Technology,2007,27(6):24-27. (in Chinese)
[20] 常雅宁,赵素芬,倪炜.竹醋液抗氧化性能研究[J].华东理工大学学报,2004,11(6):640-643. CHANG Y N,ZHAO S F, NI W. Research of the antioxidative properties of bamboo vinegar[J].Journal of East China University of Science and Technology,2004,11(6):640-643.(in Chinese)
[21] 孙艳萍,罗健英,但青. 母乳超氧化物歧化酶的动态测定及其临床意义[J].中国儿童保健杂志,2002,10(6):416-417. SUN Y P, LUO J Y, DAN Q.The clinic significance and dynamic changes of superoxide dismutase in human milk[J].Chinese Journal of Child Health Care,2002,10(6):416-417. (in Chinese)