迷迭香酸对哮喘小鼠氧化性肺损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2017.12.036

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (12): 3650-3655.doi: 10.16431/j.cnki.1671-7236.2017.12.036

迷迭香酸对哮喘小鼠氧化性肺损伤的保护作用

梁正敏¹, 廖晓光^1,2, 文雪梅¹, 徐杨峰¹, 邓鑫¹, 颜国庆¹, 何家康¹

1. 广西大学动物科学技术学院, 南宁 530005;
2. 广西畜牧研究所, 南宁 530001

收稿日期:2017-06-06 出版日期:2017-12-20 发布日期:2017-12-20
通讯作者: 何家康 E-mail:32148859@qq.com
作者简介:梁正敏(1992-),女,贵州遵义人,硕士生,研究方向:中兽医药,E-mail:277138349@qq.com
基金资助:
国家自然科学基金项目（31360624）；广西南宁市科学研究与技术开发计划项目（20163163）

Protective Effects of Rosmarinic Acid on Oxidative Lung Damage in Asthmatic Mice

LIANG Zheng-min¹, LIAO Xiao-guang^1,2, WEN Xue-mei¹, XU Yang-feng¹, DENG Xin¹, YAN Guo-qing¹, HE Jia-kang¹

1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
2. Guangxi Institute of Animal Science, Nanning 530001, China

Received:2017-06-06 Online:2017-12-20 Published:2017-12-20

摘要/Abstract

摘要：

为评价迷迭香酸对哮喘小鼠模型氧化性肺损伤的保护作用，本研究用卵清蛋白（OVA）致敏、激发雌性BALB/c小鼠建立哮喘模型，并用OVA和H₂O₂联合激发小鼠作为氧化肺损伤阳性对照模型。在最后一次滴鼻激发24 h后，取支气管肺泡灌洗液（BALF）进行细胞计数并测定活性氧（ROS）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平，取左侧肺脏固定做HE染色。结果显示，迷迭香酸可明显减少BALF中细胞总数和嗜酸性粒细胞数目，显著抑制肺组织和BALF中ROS的产生，升高SOD和GSH-Px水平，改善肺组织病理变化。本试验结果表明，迷迭香酸对氧化肺损伤起到明显的保护作用。

关键词: 迷迭香酸; 哮喘; 氧化性肺损伤; 抗氧化

Abstract:

In order to evaluate the protective effect of rosmarinic acid on oxidative lung damage in asthmatic mice, in this study, the asthma model was established by sensitizing and challenging female BALB/c mice with ovalbumin (OVA), and some mice were challenged by OVA and H₂O₂ as a positive control of oxidative lung damage. The BALF, lung tissues of mice were harvested 24 h after the final OVA challenge and the number of total cells and eosinophils were measured, ROS,SOD and GSH-Px levels were detected, and pathological changes of lung tissue were observed using HE staining. The results showed that rosmarinic acid significantly reduced the number of total cells and eosinophils in BALF, significantly inhibited the production of ROS in lung tissue and BALF, increased the levels of SOD and GSH-Px, and ameliorated pathological changes of lung tissue. The results suggested that rosmarinic acid could protect oxidative lung damage in asthmatic mice.

Key words: rosmarinic acid; asthma; oxidative lung damage; antioxidation

中图分类号:

R285.5

梁正敏, 廖晓光, 文雪梅, 徐杨峰, 邓鑫, 颜国庆, 何家康. 迷迭香酸对哮喘小鼠氧化性肺损伤的保护作用[J]. 《中国畜牧兽医》, 2017, 44(12): 3650-3655.

LIANG Zheng-min, LIAO Xiao-guang, WEN Xue-mei, XU Yang-feng, DENG Xin, YAN Guo-qing, HE Jia-kang. Protective Effects of Rosmarinic Acid on Oxidative Lung Damage in Asthmatic Mice[J]. , 2017, 44(12): 3650-3655.

参考文献

[1] Duan L, Li J, Ma P, et al. Vitamin E antagonizes ozone-induced asthma exacerbation in BALB/c mice through the Nrf2 pathway[J]. Food and Chemical Toxicology, 2017,107(Pt A):47-56.
[2] Lee I T, Yang C M. Role of NADPH oxidase/ROS in pro-inflammatory mediators-induced airway and pulmonary diseases[J]. Biochemical Pharmacology, 2012, 84(5):581-590.
[3] Nielsen F, Mikkelsen B B, Nielsen J B, et al. Plasma malondialdehyde as biomarker for oxidative stress:Reference interval and effects of life-style factors[J]. Clinical Chemistry, 1997, 43(7):1209-1214.
[4] Nadeem A, Siddiqui N, Alharbi N O, et al. Airway and systemic oxidant-antioxidant dysregulation in asthma:A possible scenario of oxidants spill over from lung into blood[J]. Pulmonary Pharmacology & Therapeutics, 2014, 29(1):31-40.
[5] ElNaa M M, ElRefaei M F, Nasif W A, et al. In-vivo antioxidant and anti-inflammatory activity of rosiglitazone, a peroxisome proliferator-activated receptor-gamma (PPAR-γ) agonists in animal model of bronchial asthma[J]. Journal of Pharmacy and Pharmacology, 2015, 67(10):1421-1430.
[6] Ho W E, Cheng C, Peh H Y, et al. Anti-malarial drug artesunate ameliorates oxidative lung damage in experimental allergic asthma[J]. Free Radical Biology and Medicine, 2012, 53(3):498-507.
[7] Dai Y, Tu F J, Yao Z H, et al. Rapid identification of chemical constituents in traditional Chinese medicine fufang preparation xianling gubao capsule by LC-linear ion trap/Orbitrap mass spectrometry[J]. The American Journal of Chinese Medicine, 2013, 41(5):1181-1198.
[8] Li S, Wang Y, Shi Y, et al. Regulatory effects of stage-treatment with established Chinese herbal formulas on inflammatory mediators in pediatric asthma[J]. Journal of Traditional Chinese Medicine, 2013, 33(6):727-732.
[9] Liang Z, Xu Y, Wen X, et al. Rosmarinic acid attenuates airway inflammation and hyperresponsiveness in a murine model of asthma[J]. Molecules, 2016, 21(6):1-15.
[10] Liang Z, Nie H, Xu Y, et al. Therapeutic effects of rosmarinic acid on airway responses in a murine model of asthma[J]. Int Immunopharmacol, 2016, 41:90-97.
[11] 付远妨, 赵尉丹, 杨潇, 等. 鸡血藤总黄酮对猪圆环病毒2型感染小鼠脾脏氧化应激的影响[J]. 中国畜牧兽医, 2017,44(5):1526-1532.
[12] 王尧,况九龙. 支气管哮喘气道重塑与氧化应激的关系[J]. 中华哮喘杂志,2008,2:133-136.
[13] Ye L, Mou Y, Wang J, et al. Effects of microRNA-19b on airway remodeling, airway inflammation and degree of oxidative stress by targeting TSLP through the Stat3 signaling pathway in a mouse model of asthma[J]. Oncotarget, 2017, 8(29):47533-47546.
[14] Frossi B, De Carli M, Piemonte M, et al. Oxidative microenvironment exerts an opposite regulatory effect on cytokine production by Th1 and Th2 cells[J]. Mol Immunol, 2008, 45(1):58-64.
[15] Topic A, Francuski D, Nikolic A, et al. The role of oxidative stress in the clinical manifestations of childhood asthma[J]. Fetal and Pediatric Pathplogy, 2017, 8:1-10.
[16] Lee K S, Kim S R, Park S J, et al. Hydrogen peroxide induces vascular permeability via regulation of vascular endothelial growth factor[J]. American Journal of Respiratory Cell and Molecular Biology, 2006, 35(2):190-197.
[17] Al-Harbi N O, Nadeem A, Al-Harbi M M, et al. Airway oxidative stress causes vascular and hepatic inflammation via upregulation of IL-17A in a murine model of allergic asthma[J]. Int Immunopharmacol, 2016, 34:173-182.
[18] Rangasamy T, Guo J, Mitzner W A, et al. Disruption of Nrf2 enhances susceptibility to severe airway inflammation and asthma in mice[J]. The Journal of Experimental Medicine, 2005, 202(1):47-59.
[19] Koike Y, Hisada T, Utsugi M, et al. Glutathione redox regulates airway hyperresponsiveness and airway inflammation in mice[J]. American Journal of Respiratory Cell and Molecular Biology, 2007, 37(3):322-329.
[20] Nadeem A, Chhabra S K, Masood A, et al. Increased oxidative stress and altered levels of antioxidants in asthma[J]. J Allergy Clin Immunol, 2003, 111(1):72-78.
[21] Nadeem A, Siddiqui N, Al-Harbi N O, et al. TLR-7 agonist attenuates airway reactivity and inflammation through Nrf2-mediated antioxidant protection in a murine model of allergic asthma[J]. Int J Biochem Cell Biol, 2016, 73:53-62.
[22] Zhang Y X, Wang L, Xiao E L, et al. Ginsenoside-Rd exhibits anti-inflammatory activities through elevation of antioxidant enzyme activities and inhibition of JNK and ERK activation in vivo[J]. Int Immunopharmacol, 2013, 17(4):1094-1100.
[23] Petersen M, Simmonds M S. Rosmarinic acid[J]. Phytochemistry, 2003, 62(2):121-125.
[24] 苏平,王根女,吴丹,等. 迷迭香酸的生理活性功能及其来源研究进展[J]. 食品与发酵工业,2008,34(12):135-138.

迷迭香酸对哮喘小鼠氧化性肺损伤的保护作用

Protective Effects of Rosmarinic Acid on Oxidative Lung Damage in Asthmatic Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王静, 宋红兵, 吴艳玲, 林威敏, 肖天放. 西红花苷在动物抗氧化作用中的研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2233-2244.
[2]	汪阳阳, 秦士贞, 唐德富, 邵玉新, 史兆国, 王铮, 李星, 赵东栋. 锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响[J]. 中国畜牧兽医, 2023, 50(6): 2276-2285.
[3]	吕春荣, 梁家充, 邵庆勇, 吴国权, 张玉梅, 权国波. 丙酮酸钠对山羊冷冻精子质量的影响[J]. 中国畜牧兽医, 2023, 50(6): 2363-2369.
[4]	李灿, 李楚楚, 曾维, 张宁, 纪春晓, 陈韬. 猪RegⅢγ蛋白的重组表达及功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2414-2426.
[5]	李永强, 包瑞莹, 王琴, 邱平飞, 李笑春, 石慧宇, 张海文, 王学梅. 蝇蛆抗氧化肽对IPEC-J2细胞氧化应激损伤的保护作用[J]. 中国畜牧兽医, 2023, 50(5): 1836-1844.
[6]	乔宏兴, 张菡, 赵圣振, 孙彦婷, 张晓静, 康宇, 边传周. 发酵杜仲叶对肉鸡生产性能、抗氧化性能和免疫相关基因表达的影响[J]. 中国畜牧兽医, 2023, 50(5): 1845-1854.
[7]	谢华亮, 雷小文, 李建军, 刘珍妮, 钟云平, 谭东海, 张强. 蚯蚓液对105~175日龄蛋鸡产蛋性能、血清生化指标、抗氧化指标及免疫相关指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1899-1906.
[8]	雷奇, 任杰, 康杰. 黄芩对ZEA暴露雌性小鼠抗氧化能力、性激素水平和ERα mRNA表达的影响[J]. 中国畜牧兽医, 2023, 50(3): 989-997.
[9]	豆佳红, 王梓颖, 杨娟, 周炜炜, 张同存, 何红鹏, 戴小枫, 李秀梅. 基于网络药理学和分子对接分析赤芍抗氧化应激作用的活性成分及其机制[J]. 中国畜牧兽医, 2023, 50(3): 1229-1240.
[10]	赵陆原, 邹晨浩, 曹久爱, 郭燕珊, 刘莉君, 占秀安, 林刚, 刘兵, 余东游. 复合有机微量元素减量添加对肉鸭血清生化、免疫功能和抗氧化能力的影响[J]. 中国畜牧兽医, 2023, 50(1): 86-96.
[11]	仲华玲, 何丛丛, 吴邦元. 饲喂苜蓿鲜草和全价颗粒饲料对兔胃抗氧化性能和肌层结构的影响[J]. 中国畜牧兽医, 2023, 50(1): 143-150.
[12]	孙愉, 张昊, 李畅洋, 陶燕子, 王秋菊. 牛源抗氧化芽孢杆菌的分离鉴定及生物特性研究[J]. 中国畜牧兽医, 2023, 50(1): 359-367.
[13]	王帅杰, 谭子涵, 李晗博, 孙香雪, 艾思含, 王蕾, 赵迪, 侯永清. 槲皮素对PEDV感染幼龄仔猪肠道形态、抗氧化功能及空肠脂质代谢基因表达的影响[J]. 中国畜牧兽医, 2022, 49(9): 3391-3399.
[14]	魏佳, 柏琴, 罗晓林, 官久强, 安添午, 赵洪文, 谭武, 李华德, 谢荣清, 沙泉, 江明锋, 张翔飞. 不同断奶模式对犊牦牛生长发育、血清生化指标及抗氧化能力的影响[J]. 中国畜牧兽医, 2022, 49(9): 3400-3410.
[15]	李一鹏, 张虎, 任娟, 任欣, 刘聚祥, 王庚南. 紫锥菊根中菊苣酸提取工艺及对氧化应激肠道细胞的保护作用研究[J]. 中国畜牧兽医, 2022, 49(7): 2497-2505.