鸭腺病毒A型Hexon基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2017.10.030

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (10): 3042-3048.doi: 10.16431/j.cnki.1671-7236.2017.10.030

鸭腺病毒A型Hexon基因克隆与序列分析

万春和, 刘荣昌, 陈翠腾, 程龙飞, 傅光华, 傅秋玲, 施少华, 陈红梅, 黄瑜

福建省农业科学院畜牧兽医研究所, 福建省畜禽疫病防治工程技术研究中心, 福建省禽病防治重点实验室, 福州 350013

收稿日期:2017-04-17 出版日期:2017-10-20 发布日期:2017-10-20
通讯作者: 黄瑜 E-mail:huangyu_815@163.com
作者简介:万春和(1982-),男,湖北鄂州人,博士,副研究员,研究方向:水禽病毒分子生物学,E-mail:chunhewan@126.com
基金资助:
国家水禽产业技术体系（CARS-43）；国家自然科学基金（31602068）；福建省农业科学院畜牧兽医研究所基金[MYQJ2015（S）-3]；福建省自然科学基金（2015J01114）；福建省农业科学院"青年科技英才百人计划"专项（YC2015-12）

Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A

WAN Chun-he, LIU Rong-chang, CHEN Cui-teng, CHENG Long-fei, FU Guang-hua, FU Qiu-ling, SHI Shao-hua, CHEN Hong-mei, HUANG Yu

Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fujian Animal Diseases Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

Received:2017-04-17 Online:2017-10-20 Published:2017-10-20

摘要/Abstract

摘要：

为明确鸭腺病毒A型Hexon基因特征及其在腺病毒科病毒分类中的作用，本研究从前期分离鉴定的一株番鸭源鸭腺病毒A型分离株（JX2016株）中分段扩增获得其Hexon基因编码区。结果发现，鸭腺病毒A型JX2016株Hexon基因编码区为2 733 bp，编码910个氨基酸，与鸭腺病毒A型（FJ12025株）核苷酸同源性最高，达99.8%；与其他分离株Hexon基因核苷酸同源性均在99.4%以上；与鸭腺病毒2型（GR株，未明确分类地位）同源性仅为54.5%；与禽腺病毒A型Phelps株（鸡源）及P29株（鹅源）的核苷酸同源性分别为53.6%和55.2%。遗传进化树分析发现，所有鸭腺病毒A型分离株在遗传进化上均处于相同的亚分支，且与绵羊腺病毒D型（OAV287株）均处于富AT腺病毒属遗传进化分支。但鸭腺病毒2型（GR株）与禽腺病毒A型Phelps株（鸡源）、P29株（鹅源）却处于同一遗传进化分支，均属于禽腺病毒属遗传进化分支。基于Hexon蛋白的遗传进化分析，建议将鸭腺病毒A型重新命名为鸭源富AT腺病毒A型，将鸭腺病毒2型重新命名为鸭源禽腺病毒。

关键词: 鸭腺病毒A型; Hexon基因; 序列分析; 遗传进化; 分类

Abstract:

In order to clarify the characteristics of the Hexon gene and the classification candidate of duck adenovirus A under the Adenoviridae family, the Hexon gene fragments of duck adenovirus A (designated as strain JX2016) were amplified. The results demonstrated that the cloned Hexon gene of duck adenovirus A (strain JX2016) was 2 733 bp in length, coding 910 amino acids. Nucleotide homology comparison showed that the strain JX2016 shared the highest nucleotide (99.8%) homology with reference strain FJ12025, also displayed higher than 99.4% nucleotide homology with other duck adenovirus A reference strains. However, the nucleotide homologies with strain GR (duck adenovirus 2, unclassified virus), Phelps (fowl adenovirus A) and P29 (goose adenovirus) were 54.5%, 53.6% and 55.2%, respectively. The genetic evolution analysis showed that all the duck adenovirus A strains were at the same subgroup, under the same Atadenovirus genus branch with ovine atadenovirus D (strain OAV287). Meanwhile, the duck adenovirus 2 (strain GR) at the same branch under Aviadenovirus genus branch with fowl adenovirus A (strain Phelps) and goose adenovirus (strain P29). Based on the Hexon protein genetic evolution analysis, we suggested renamed the duck adenovirus A as duck-origin atadenovirus and renamed the duck adenovirus 2 as duck-origin aviadenovirus.

Key words: duck adenovirus A; Hexon gene; sequence analysis; genetic evolution; classification

中图分类号:

S852.65

万春和, 刘荣昌, 陈翠腾, 程龙飞, 傅光华, 傅秋玲, 施少华, 陈红梅, 黄瑜. 鸭腺病毒A型Hexon基因克隆与序列分析[J]. 《中国畜牧兽医》, 2017, 44(10): 3042-3048.

WAN Chun-he, LIU Rong-chang, CHEN Cui-teng, CHENG Long-fei, FU Guang-hua, FU Qiu-ling, SHI Shao-hua, CHEN Hong-mei, HUANG Yu. Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A[J]. , 2017, 44(10): 3042-3048.

参考文献

[1] Tan B, Yang X L, Ge X Y,et al. Novel bat adenoviruses with low G+C content shed new light on the evolution of adenoviruses[J]. J Gen Virol, 2017, 98(4):739-748.
[2] 陈娜娜,向冬喜,郑丛龙.腺病毒及其研究进展[J].大连医科大学学报,2010,32(5):586-590.
[3] 索南卓玛,增巴.Ⅰ群禽腺病毒套式PCR检测方法的建立及应用[J].中国畜牧兽医,2013,40(8):30-33.
[4] 高文娟,金玉,段招军.人腺病毒的研究进展[J].病毒学报,2014,30(2):193-200.
[5] Counihan K L, Skerratt L F, Franson J C, et al. Phylogenetic and pathogenic characterization of novel adenoviruses isolated from long-tailed ducks (Clangula hyemalis)[J]. Virology, 2015, 485:393-401.
[6] Zhang X, Zhong Y, Zhou Z, et al. Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014[J]. Virology, 2016, 493:12-21.
[7] 汪镇南,唐青海,颜爱,等.猪腺病毒3型Hexon蛋白多克隆抗体制备与免疫活性鉴定[J].中国畜牧兽医,2015,42(1):1-8.
[8] 刘雪美,秦建如,李育豪,等.Ⅱ群禽腺病毒五邻体蛋白单因子血清的制备[J].中国畜牧兽医,2013,40(10):16-20.
[9] 孙锦,李秋艳,李云龙,等.禽腺病毒QU株一个复制非必需区的鉴定[J].生物工程学报,2008,24(7):1263-1267.
[10] Fu G, Chen H, Huang Y, et al. Full genome sequence of egg drop syndrome virus strain FJ12025 isolated from Muscovy duckling[J]. Genome Announc, 2013, 1(4):e00623-13.
[11] Khatri A, Both G. Identification of transcripts and promoter regions of ovine adenovirus OAV287[J]. Virology, 1998, 245:128-141.
[12] Chiocca S, Kurzbauer R, Schaffner G, et al. The complete DNA sequence and genomic organization of the avian adenovirus CELO[J]. J Virol, 1996, 70(5):2939-2949.
[13] Kaján G L, Davison A J, Palya V, et al. Genome sequence of a waterfowl aviadenovirus, goose adenovirus 4[J]. J Gen Virol, 2012, 93(11):2457-2465.
[14] Kovács G M, LaPatra S E, D'Halluin J C, et al. Phylogenetic analysis of the hexon and protease genes of a fish adenovirus isolated from white sturgeon (Acipenser transmontanus) supports the proposal for a new adenovirus genus[J]. Virus Res, 2003, 98(1):27-34.
[15] Kovács G M, Davison A J, Zakhartchouk A N, et al. Analysis of the first complete genome sequence of an Old World monkey adenovirus reveals a lineage distinct from the six human adenovirus species[J]. J Gen Virol, 2004, 85(Pt 10):2799-2807.
[16] Davison A J, Wright K M, Harrach B. DNA sequence of frog adenovirus[J]. J Gen Virol, 2000, 81(10):2431-2439.
[17] Marek A, Kaján G L, Kosiol C, et al. Complete genome sequences of pigeon adenovirus 1 and duck adenovirus 2 extend the number of species within the genus aviadenovirus[J]. Virology, 2014, 462-463:107-114.
[18] Saif Y M. Diseases of Poultry[M]. 12th Edition. Blackwell Publishing, 2008.
[19] Joubert H W, Aitchison H, Maartens L H, et al. Molecular differentiation and pathogenicity of aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa[J]. J S Afr Vet Assoc, 2014, 85(1):1058.
[20] Schachner A, Marek A, Grafl B, et al. Detailed molecular analyses of the hexon loop-1 and fibers of fowl aviadenoviruses reveal new insights into the antigenic relationship and confirm that specific genotypes are involved in field outbreaks of inclusion body hepatitis[J]. Vet Microbiol, 2016, 186:13-20.
[21] Grgic H, Krell P J, Nany E. Comparison of fiber gene sequences of inclusion body hepatitis (IBH) and non-IBH strains of serotype 8 and 11 fowl adenoviruses[J]. Virus Genes, 2014, 48(1):74-80.
[22] Gu L, Krendelchtchikova V, Krendelchtchikov A, et al. Adenoviral vectors elicit humoral immunity against variable loop 2 of clade C HIV-1 gp120via "Antigen Capsid-Incorporation" strategy[J]. Virology, 2016, 487:75-84.
[23] 陈峰,招丽婵,覃健萍,等.应用宏基因组学鉴定国内第一株鸭2型腺病毒[J].中国预防兽医学报,2015,37(12):903-907.

鸭腺病毒A型Hexon基因克隆与序列分析

Cloning and Sequence Analysis of Hexon Gene of Duck Adenovirus A

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