广东省4株PCV2型分离株全基因组进化分析

doi:10.16431/j.cnki.1671-7236.2022.03.023

摘要/Abstract

摘要： 【目的】了解目前广东省猪圆环病毒2型（Porcine circovirus type 2，PCV2）分离株的基因型和进化特征，为广东省PCV2防控及疫苗株的筛选提供参考依据。【方法】运用PCR方法将4份鉴定为PCV2阳性样品进行PCV2全基因组序列扩增、测序及遗传进化分析；利用MegAlign软件对4株PCV2广东分离株ORF2氨基酸序列与国内外参考毒株进行关键位点氨基酸变异分析；应用DNAStar中Protean软件的Jameson-Wolf方法对4株PCV2广东分离株ORF2基因编码的Cap蛋白与4株疫苗株进行抗原指数预测分析。【结果】测序结果表明，4株PCV2广东分离株序列长度均为1 767 bp。遗传进化树结果表明，4株分离株均属于PCV2d基因型，且核苷酸相似性在97.7%~99.1%之间，与国内外54株参考毒株相似性在91.7%~99.8%之间，其中与PhuTho/G40312/2018株（Viet Nam，登录号：LC602996）、QZ1410株（江苏，登录号：MG732832）、GXBB1501211株（广西，登录号：MH756609）亲缘关系最为接近。关键氨基酸位点变异分析表明，在Cap蛋白上有8个氨基酸特异性突变位点，分别是Y3C、F8Y、T56S、R116K、V123I、K164E、R169G及T216A。抗原指数分析发现，广东省分离株的Cap蛋白抗原指数与4株疫苗株相比差异较大，主要集中在第7－12、47－53、80－90、160－170和205－213位氨基酸这5个区域。【结论】从广东省部分地区分离的4株PCV2均为2d亚型，其Cap蛋白氨基酸序列存在多个突变位点，抗原指数与疫苗株差异较大，提示广东省PCV2的流行趋势逐渐以2d亚型为主。

关键词: 猪圆环病毒2型(PCV2); 基因型; ORF2基因; 抗原指数; 遗传进化分析

Abstract: 【Objective】 This study was aimed to investigate the genotypes and evolutionary characteristics of Porcine circovirus type 2 (PCV2) isolated from Guangdong province, and provide reference for PCV2 prevention and control and vaccine strain screening in Guangdong province.【Method】 PCV2 whole genome sequences from 4 positive samples were amplified by PCR, and then sequenced and phylogenetically analyzed.MegAlign software was used to analyze the key variation of ORF2 amino acid sequences of 4 Guangdong isolates of PCV2 and reference strains at home and abroad.The capsid protein encoded by ORF2 gene of 4 Guangdong isolates of PCV2 and 4 vaccine strains were predicted by Jameson-Wolf method of DNAStar Protean software.【Result】 The sequence length of 4 Guangdong isolates of PCV2 was 1 767 bp.Phylogenetic tree analysis showed that all the 4 isolates belonged to PCV2d genotype with nucleotide homology of 97.7%-99.1%, 91.7%-99.8% with 54 reference strains at home and abroad, PhuTho/G40312/2018 (Viet Nam, accession No.:LC602996), QZ1410 (Jiangsu, accession No.:MG732832) and GXBB1501211 (Guangxi, accession No.:MH756609) had similar genetic relationships.Analysis of key amino acid variation showed that there were 8 amino acid specific mutation sites were found in Cap protein, which were Y3C, F8Y, T56S, R116K, V123I, K164E, R169G and T216A.Antigen analysis showed that the Cap protein antigenic index of Guangdong isolates was significantly different from that of the four vaccine strains, mainly in the amino acid regions of 7-12, 47-53, 80-90, 160-170 and 205-213.【Conclusion】 In this study, 4 strains of PCV2 isolated from parts of Guangdong province were all 2d subtypes, and there were many mutation sites in the amino acid sequence of Cap protein, and the antigenic index was also significantly different from that of the vaccine strain, suggesting that the epidemic trend of PCV2 in Guangdong province was gradually dominated by 2d subtypes.

Key words: Porcine circovirus type 2(PCV2); genotype; ORF2 gene; antigen index; genetic evolutionary analysis

中图分类号:

S852.65

羊露露, 李安琪, 袁生, 黄良宗, 黄淑坚, 温峰, 郭锦玥. 广东省4株PCV2型分离株全基因组进化分析[J]. 中国畜牧兽医, 2022, 49(3): 1015-1023.

YANG Lulu, LI Anqi, YUAN Sheng, HUANG Liangzong, HUANG Shujian, WEN Feng, GUO Jinyue. Complete Genome Evolution Analysis of Four Porcine Circovirus Type 2 Isolates from Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1015-1023.

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