《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (9): 2603-2612.doi: 10.16431/j.cnki.1671-7236.2017.09.010

• 动物营养与饲料科学 • 上一篇    下一篇

断奶日龄对舍饲羔羊小肠内容物中主要消化酶活性的影响

郭江鹏1,2, 王俊1, 李发弟2,3, 李海静2,4, 马腾2,5, 齐志国1, 郝正里2, 陈京媛1   

  1. 1. 北京市畜牧总站, 北京 100107;
    2. 甘肃农业大学动物科学技术学院, 兰州 730070;
    3. 兰州大学草地农业科技学院, 兰州 730030;
    4. 山东东阿阿胶股份有限公司, 聊城 252000;
    5. 大同市良种奶牛有限责任公司, 大同 037000
  • 修回日期:2017-02-22 出版日期:2017-09-20 发布日期:2017-09-22
  • 通讯作者: 李发弟 E-mail:lifd@lzu.edu.cn
  • 作者简介:郭江鹏(1972-),男,陕西铜川人,博士,高级畜牧师,研究方向:反刍动物营养,E-mail:guojp72@163.com
  • 基金资助:

    现代农业产业技术体系建设专项资金;甘肃省自然科学基金(3ZS061-A25-077);甘肃省教育厅高等学校研究生导师科研项目(0702-07)

Effect of Different Weaning Ages on Main Digestive Enzymes Activities in the Small Intestinal Contents of Lamb

GUO Jiang-peng1,2, WANG Jun1, LI Fa-di2,3, LI Hai-jing2,4, MA Teng2,5, QI Zhi-guo1, HAO Zheng-li2, CHEN Jing-yuan1   

  1. 1. Beijing General Station of Animal Husbandry Service, Beijing 100107, China;
    2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    3. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730030, China;
    4. Shandong Dong E E Jiao Co., Ltd., Liaocheng 252000, China;
    5. Datong Liangzhong Dairy Co., Ltd., Datong 037000, China
  • Revised:2017-02-22 Online:2017-09-20 Published:2017-09-22

摘要:

为研究早期断奶日龄对羔羊小肠各段pH及主要消化酶活性的影响,选用55只初生重为(3.43±1.15)kg的甘肃现代肉羊育种群哺乳公羔,按同质原则分成3组,即对照组(正常吮乳组,25只)、Ⅰ组(28日龄断奶组,15只)和Ⅱ组(42日龄断奶组,15只)。在断奶12 h、7 d和14 d后从每个处理中随机抽取5只羔羊屠宰,测定小肠各段pH、α-淀粉酶、胰蛋白酶、糜蛋白酶和脂肪酶活性变化。结果显示,十二指肠食糜能够更为敏感地表达断奶对小肠pH及主要消化酶活性的影响。从小肠近端至远端,断奶对各肠段内容物pH及主要消化酶活性的影响逐渐减弱;断奶对小肠内容物pH、胰蛋白酶活性和脂肪酶活性影响较小;断奶日龄对羔羊小肠主要消化酶活性恢复所需要的时间影响不大,28日龄断奶组小肠α-淀粉酶活性恢复时间较42日龄断奶组短。本试验条件下,对羔羊实施28日龄断奶可行,且羔羊小肠主要消化酶活性恢复能力不逊于42日龄断奶羔羊。

关键词: 羔羊; 断奶日龄; 小肠内容物; pH; 消化酶

Abstract:

The objective of this study was to determine the effects of different weaning ages on pH and the main digestive enzymes activities in the small intestinal contents of lamb. Fifty-five male lambs of Gansu modern breeding sheep group with(3.43±1.15)kg initial body weight were divided into 3 treatments randomly that were control group (sucking,25 lambs), group Ⅰ (weaning at 28 days old, 15 lambs), and group Ⅱ(weaning at 42 days old,15 lambs).5 lambs from each group were slaughtered at 12 h,7 d and 14 d after weaning,respectively,and the the change of pH and activities of α-amylase,trypsin,chympotrypsin and lipase were measured in the small intestinal contents. The results showed that the effects of pH and the main digestive enzymes activities could be expressed sensitively in the contents of duodenum. From duodenum to ileum,the effects of weaning turned weakening gradually,and it did not show a high-impact on pH,trypsin and lipase activities. There was no significant influence in the recovery time to the normal levels of the main digestive enzymes activities in the small intestinal contents by different weaning ages,the recovery time of α-amylase in group Ⅰ was shorter than group Ⅱ. In this trail condition,it was feasible to wean at 28 days of age,and it had no significant difference in the recovery ability of main digestive enzymes in the small intestinal contents weaning at 28 and 42 days old.

Key words: lamb; weaning day of age; small intestinal content; pH; digestive enzyme

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