新疆猪源沙门氏菌耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2017.03.038

摘要/Abstract

摘要：

为了解新疆某规模化养殖场猪源沙门氏菌对临床上常用抗菌药物的耐药情况，以及β-内酰胺酶、16S rRNA甲基化酶和质粒介导的喹诺酮耐药（plasmid mediated quinolone resistance，PMQR）基因的流行情况，本试验通过琼脂稀释法对分离的菌株进行最小抑菌浓度测定，PCR方法进行β-内酰胺酶bla_TEM、bla_CMY-2、bla_CTX-M、bla_LAP-1、bla_KPC、bla_OXA和bla_SHV基因，16S rRNA甲基化酶基因armA和rmtB及PMQR类基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA、oqxB和aac（6'）-Ib-cr的检测，确定阳性菌株，分析其携带的基因型与耐药表型之间的关系。分离的猪源沙门氏菌对环丙沙星和安普霉素耐药率最高，均为77.9%（102/131），耐药菌主要以8耐为主（29.0%，38/131）。从被检基因中检测出11种耐药基因，不同基因共存有24种类型，主要以bla_TEM+bla_OXA+qnrS+aac（6'）-Ib-cr+oqxA+oqxB（27.6%，32/116）；bla_TEM+bla_OXA+qnrS+oqxA+oqxB（24.1%，28/116）；bla_TEM+qnrS（18.0%，21/116）形式共存。该养殖场分离的沙门氏菌耐药现象严重，分离耐药菌株存在β-内酰胺酶、16S rRNA甲基化酶和PMQR类基因共存现象，提示应加强对β-内酰胺酶、16S rRNA甲基化酶和PMQR类因子的监控。

关键词: 猪源沙门氏菌; 耐药性; 耐药基因; 检测

Abstract:

The objective of this study was to investigate the resistance of Salmonella and prevalence of resistance genes isolated from a pig farm in Xinjiang, and their coexistence with the major β-lactamases, 16S rRNA methylation enzyme genes and PMQR. The minimum inhibitory concentrations of Salmonella isolated from the pig farms were determined by agar dilution method, PCR was used to detect bla_TEM, bla_CMY-2, bla_CTX-M, bla_LAP-1, bla_KPC, bla_OXAand bla_SHV genes, 16S rRNA methylation enzyme genes armA and rmtB, and PMQR including qnrA, qnrB, qnrC, qnrD, qnrS, qepA, oqxA, oqxB and aac(6')-Ib genes. The positive strains were performed by using DNA sequencing to determine the purpose of the belt. The result showed that resistant rate of Salmonella isolates from swine were highest to ciprofloxacin and apramycin sulfate (77.9%, 102/131). Resistant mainly based on 8 kinds of drug resistance (29.0%, 38/131), 11 kinds of resistance genes were detection, the coexistence of different genotypes had 24 types. The main types were bla_TEM+bla_OXA+qnrS+aac(6')-Ib-cr+oqxA+oqxB (27.6%, 32/116), bla_TEM+bla_OXA+qnrS+oqxA+oqxB (24.1%, 28/116) and bla_TEM+qnrS (18.0%, 21/116). The Salmonella isolated from the farm had phenomenon seriously resistance, it coexisted with the main β-lactamase, 16S rRNA methylation enzyme genes and PMQR factors. The result suggested that it should strengthen monitoring to the β-lactamase enzymes, 16S rRNA methylation enzyme genes and PMQR factors.

Key words: Salmonella of swine; resistance; resistance genes; detection

中图分类号:

S852.61

江萍, 关茹飞, 夏利宁, 林亚军. 新疆猪源沙门氏菌耐药性及耐药基因检测[J]. 《中国畜牧兽医》, 2017, 44(3): 896-903.

JIANG Ping, GUAN Ru-fei, XIA Li-ning, LIN Ya-jun. Detection of Resistance and Resistance Genes of Salmonella from Swine in Xinjiang[J]. , 2017, 44(3): 896-903.

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