肉鸡鸡胚肝脏中脂滴的发育研究

doi:10.16431/j.cnki.1671-7236.2017.03.011

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (3): 692-697.doi: 10.16431/j.cnki.1671-7236.2017.03.011

肉鸡鸡胚肝脏中脂滴的发育研究

余燕¹, 马艺源², 吴世秀¹, 陶立奎², 张艳红¹, 徐之勇¹, 马金友¹

1. 河南科技学院动物科技学院, 新乡 453003;
2. 河南师范大学附属中学, 新乡 453002

收稿日期:2016-05-19 出版日期:2017-03-20 发布日期:2017-03-21
通讯作者: 马金友 E-mail:marsjy@163.com
作者简介:余燕(1972-),女,河南信阳人,博士,副教授,研究方向:动物组织胚胎学与发育生物学,E-mail:yymtt03@163.com
基金资助:
河南省高校科技创新人才支持计划（13HASTIT006）；河南省科技攻关项目（162102110038）；河南省教育厅自然科学基础研究项目（14A230011）

Study on Development of Broiler Liver Lipid Droplet during Embryonic Period

YU Yan¹, MA Yi-yuan², WU Shi-xiu¹, TAO Li-kui², ZHANG Yan-hong¹, XU Zhi-yong¹, MA Jin-you¹

1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China;
2. The Affiliated Middle School of Henan Normal University, Xinxiang 453002, China

Received:2016-05-19 Online:2017-03-20 Published:2017-03-21

摘要/Abstract

摘要：

为了探索鸡胚肝脏脂滴的发育规律，选取120枚受精蛋，分别在孵化的第7~21天（E7~E21）每天随机选取6枚蛋，收集鸡胚，采集鸡胚肝脏，采用冰冻切片、苏丹Ⅲ染色观察鸡胚肝脏脂滴的形成和发育特点。结果发现：①鸡胚发育到E9胚龄，肝细胞内开始出现脂滴，且随着胚龄增加，脂滴的数量不断增多，脂滴的大小不断增大；②E18胚龄以前，肝脏脂滴的分布从中央静脉开始沿肝索到肝小叶边缘数量逐渐减少，E18胚龄以后，肝脏边缘脂滴数量较肝脏中央的多；③肝脏脂滴的发育分为E9~E14、E15~E17和E18~E21 3个阶段，而且后一个阶段脂滴的数量和体积均显著大于前一个阶段（P<0.05），脂滴的平均大小在E9~E14、E15~E17和E18~E21 3个阶段分别为3.2、5.7和10.8 μm，平均数量分别为67、327、397个/mm²。以上结果表明，随着鸡胚的发育，肝细胞内脂滴数量增加、体积变大。

关键词: 肉鸡鸡胚; 脂滴; 肝脏; 发育

Abstract:

This study was aimed to explore the developmental change of lipid droplet in liver of chicken embryos.120 fertilized eggs were incubated, and 6 embryos were selected randomly every day and their livers were removed for measuring the formation and development of lipid droplets from E7 to E21 during the incubation.The methods of frozen section and Sudan Ⅲ staining were used. The results showed that:①Lipid droplets emerged early on the 9th day of chicken embryos incubation (E9). The quantities and sizes of lipid droplets became more and larger with the increasing of chicken embryos age. ②Quantities of lipid droplets in every hepatic lobule decreased from central veins to the margin of hepatic lobule along hepatic cords before E18, while more lipid droplets distributed at the margin of the liver than that in the center of liver after E18. ③The quantities and sizes of hepatic lipid droplets were significant difference during the three stage of E9-E14, E15-E17 and E18-E21 (P<0.05). The average sizes of hepatic lipid droplets were 3.2, 5.7 and 10.8 μm, respectively, and their average numbers were 67,327 and 397 n/mm², respectively. In conclusion, the quantities and sizes of hepatic lipid droplets became more and larger with the increasing of chicken embryos age.

Key words: chicken embryos; lipid droplet; liver; development

中图分类号:

S831.1

余燕, 马艺源, 吴世秀, 陶立奎, 张艳红, 徐之勇, 马金友. 肉鸡鸡胚肝脏中脂滴的发育研究[J]. 《中国畜牧兽医》, 2017, 44(3): 692-697.

YU Yan, MA Yi-yuan, WU Shi-xiu, TAO Li-kui, ZHANG Yan-hong, XU Zhi-yong, MA Jin-you. Study on Development of Broiler Liver Lipid Droplet during Embryonic Period[J]. , 2017, 44(3): 692-697.

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肉鸡鸡胚肝脏中脂滴的发育研究

Study on Development of Broiler Liver Lipid Droplet during Embryonic Period

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