新疆艾比湖鹅喉羚mtDNA D-Loop区序列多态性研究

doi:10.16431/j.cnki.1671-7236.2016.05.029

《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (5): 1316-1322.doi: 10.16431/j.cnki.1671-7236.2016.05.029

新疆艾比湖鹅喉羚mtDNA D-Loop区序列多态性研究

塔吉古丽·吐热甫, 艾孜孜江·乃比, 宁礼群, 马合木提·哈力克

新疆大学生命科学与技术学院, 乌鲁木齐 830046

收稿日期:2015-10-26 出版日期:2016-05-20 发布日期:2016-05-23
通讯作者: 马合木提·哈力克 E-mail:mahmuthalic@xju.edu.cn
作者简介:塔吉古丽·吐热甫(1990-),女,新疆库尔勒人,硕士生,研究方向:保护遗传学,E-mail:1125598762@qq.com
基金资助:
国家自然科学基金项目(31360266);中华人民共和国全球环境基金艾比湖流域可持续管理与生物多样性保护项目(zx-3-1)

Genetic Diversity of Gazella subgutturosa Based on Mitochondrial DNA D-Loop Sequence in Xinjiang Ebinur Lake

TAJIGUL Turap, EZIZJAN Nabi, NING Li-qun, MAHMUT Halik

College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China

Received:2015-10-26 Online:2016-05-20 Published:2016-05-23

摘要/Abstract

摘要： 为探讨新疆艾比湖鹅喉羚(Gazella subgutturosa)遗传多样性和遗传背景,本试验对艾比湖鹅喉羚35份粪便样本进行了研究,采用非损伤性DNA分析技术,扩增其mtDNAD-Loop区878bp片段序列,测序结果进行GenBank数据库的BLAST比对及ClustalW和DNASP软件分析。结果表明,成功扩增出以上片段序列,共检测到55个多态性位点,其中未发现缺失、插入现象;发现15个变异位点,包括14个转换位点、1个颠换位点。A、T、C、G含量分别为28.03%、31.57%、24.84%、15.56%,A+T的含量(59.6%)高于C+G含量(40.4%),具有明显的碱基组成偏向性。除此之外共测到16种单倍型,单倍型多态性(h)为0.886±0.037,核苷酸多态性(π)为0.037±0.01528。表明中国新疆艾比湖国家级自然保护区鹅喉羚mtDNAD-Loop区序列存在丰富的多态性。

关键词: 艾比湖; 鹅喉羚; 种群数量; mtDNA D-Loop; 遗传多样性

Abstract: To investigate the genetic diversity and genetic data of Gazella subgutturosa from Xinjiang Ebinur lake National Wetland Nature Reserves, the Ebinur lake goose antelope 35 feces samples were analyzed.Using noninvasive DNA technology, the mitochondrial DNA D-Loop 878 bp fragment was successfully amplified.Sequences results for GenBank database BLAST comparison and Clustal W and DNASP software analysis.The results showed that the sequences of the above fragments were successfully amplified, total 55 polymorphic loci were detected, which were not found insert and deficiency.This study found 15 variable sites, including 14 transversion sites and 1 top transposition.The average A, T, C and G contents in D-Loop were 28.03%, 31.57%, 24.84% and 15.56%, respectively.The contents of A+T (59.6%) were higher than G+C (40.4%).In addition, a total of 16 mtDNA haploid type were detected, haploid type polymorphism (h) was 0.886 ±0.037, nucleotide diversity degree (π) was 0.037±0.01528.The results showed that Ebinur lake Gazelle subgutturosa maintained high variation in mitochondrial D-Loop sequences.

Key words: Ebinur lake; Gazelle subgutturosa; populations; mtDNA D-Loop; genetic diversity

中图分类号:

S813.3

塔吉古丽·吐热甫, 艾孜孜江·乃比, 宁礼群, 马合木提·哈力克. 新疆艾比湖鹅喉羚mtDNA D-Loop区序列多态性研究[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(5): 1316-1322.

TAJIGUL Turap, EZIZJAN Nabi, NING Li-qun, MAHMUT Halik. Genetic Diversity of Gazella subgutturosa Based on Mitochondrial DNA D-Loop Sequence in Xinjiang Ebinur Lake[J]. , 2016, 43(5): 1316-1322.

参考文献

[1] Kingswood S C,Blank D A.Gazella subgutturosa[J].Mammalian Species,1996,518:1-10.
[2] 孙铭娟,高行宜,阿不力米提·阿不都卡迪尔,等.鹅喉羚(Gazella subgutturosa)研究动态[J].干旱区研究,2002,19(3):75-80.
[3] Haque M N,Smith T R.Reintroduction of Arabian sand gazelles Gazelle subguttrosa marica in Saudi Arabia[J].Boil Conserv,1996,76(2):203-207.
[4] 高行宜,姚军.新疆哈密盆地初冬鹅喉羚的地理分布与种群数量[J].干旱区地理,2006,29(2):213-218.
[5] 吕雪峰,阿布力孜·阿布力米提,宫平,等.鹅喉羚线粒体D-Loop和Cytb序列遗传多样性分析[J].生物技术,2015,25(2):165-184.
[6] Hayatgheib D,Karami M,Farahmand H,et al.Morpho-logical study and comparison of western and eastern goitered gazelle(Gazella subgutturosa:Gueldenstaedt,1780) populations in Iran[J].Int J Environ Res,2011,5(1):225-232.
[7] Xia C J,Xu W X,Yang W K,et al.Seasonal and sexual variation in vigilance behavior of goitered gazelle (Gazella subgutturosa) in western China[J].J Ethol,2011,29(3):443-451.
[8] Xu W X,Xia C J,Lin J,et al.Diet of Gazella subgutturosa (Guldenstaedt,1780) and food overlap with domestic sheep in Xinjiang,China[J].Folia Zool,2012,61(1):54-60.
[9] Blank D,Ruckstuhl K,Yang W.Influence of population density on group sizes in goitered gazelle (Gazella subgutturosa Guld,1780)[J].Eur J Wildl Res,2012,58(6):981-989.
[10] Murtskhvaladze M,Gurielidze Z,Kopaliani N,et al.Gene introgression betweenGazella subgutturosa and G.marica:Limitations of maternal inheritance analysis for species identification with conservation purposes[J].Acta Theriol,2012,57(4):383-386.
[11] 李莹,徐文轩,杨维康,等.卡拉麦里山有蹄类自然保护区鹅喉羚生境适宜性评价[J].兽类学报,2010,30(1):11-20.
[12] 徐文轩,乔建芳,刘伟,等.鹅喉羚生态生物学研究现状[J].生态学杂志,2008,27(2):257-262.
[13] 於建国,陈静波,多来提尼牙孜·依明,等.和田黑鸡mtDNA控制区D-Loop序列分析[J].中国畜牧兽医,2011,38(7):105-107.
[14] 敦松长.牦牛遗传多样性、起源驯化及分类学地位研究-线粒体DNA D-Loop序列分析[D].西宁:中国科学院西北高原生物研究所,2006.
[15] Sorokin P A,Soldatova N V,Lukarevskiy V S,et al.Genetic diversity and relations of the goitered gazelle (Gazella subgutturosa) groups from Uzbekistan,Turkmenistan,and Azerbaijan:Analysis of mitochondrial DNA[J].Biology Bulletin,2011,38(6):585-590.
[16] Bandelt H J,Forster P,Rohl A.Median-joining networks for inferring intraspecific phylogenies[J].Mol Biol Evol,1999,16(1):37-48.
[17] Tamura K,Stecher G,Peterson D,et al.Mega 6:Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution,2013,30(4):2725-2729.
[18] Tamura K,Peterson D,Peterson N,et al.Mega 5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
[19] Waits L,Paetkau D.Noninvasive genetic sampling tools for wildlife biologists:A review of applications and recommendations for accurate data collection[J].Journal of Wildlife Management, 2005,69(4):1419-1433.
[20] Waits L P.Using non-invasive genetic sampling to detect and estimate abundance of rarewild life species[J].Molecular Ecology,2004,10:249-256.
[21] 阿地力江·卡德尔,恩特马克,曹杰,等.普氏野马线粒体DNA D-Loop区序列多态性研究[J].生物技术,2008,18(4):4-7.
[22] Irwin D M,Kocher T D,Wilson A C.Evolution of the cytochrome b gene of mammals[J].Journal of Molecular Evolution.1991,32(2):128-144.
[23] 吴兰苏,杨爱珍,刘和,等.线粒体调控细胞凋亡的研究进展[J].中国农学报,2010,26(8):63-68.
[24] Zhou H,Li D Q,Zang Y G,et al.Study on mitochondrial DNA genetic diversity of Tibet an antelope[J].Hereditas,2006,28(3):299-305.
[25] Neigel J E,Avise J C.Application of a random walk model to geographic distributions of animal mitochondrial DNA variation[J].Genetics,1993,135(4):1209-1220.
[26] 艾萨江·图合提.基于线粒体DNA D-Loop区的新疆鹅喉羚种群遗传结构和遗传多态性研究[D].乌鲁木齐:新疆大学,2013.
[27] 艾孜孜江·乃比,夏米西丁·阿不都热依木,热木图拉·阿卜杜克热木,等.艾比湖国家级湿地自然保护区鹅喉羚种群数量与分布[J].四川动物,2015,3:447-452.

新疆艾比湖鹅喉羚mtDNA D-Loop区序列多态性研究

Genetic Diversity of Gazella subgutturosa Based on Mitochondrial DNA D-Loop Sequence in Xinjiang Ebinur Lake

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	熊陈勇, 尹彦文, 施开创, 李军, 郑敏, 韦显凯, 冯淑萍, 龙凤, 屈素洁, 陆文俊, 周洪槿, 黄海莲, 谢守玉, 黎宗强. 2019-2021年鸭坦布苏病毒广西流行毒株遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(8): 3099-3111.
[2]	彭彩淳, 李斌强, 王野影, 粟海军. 基于线粒体Cytb基因的贵州野猪(Sus scrofa) 群体遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(7): 2601-2612.
[3]	张强, 洛桑顿珠, 巴桑旺堆, 彭阳洋, 俄广鑫, 尼玛次仁, 索朗多吉, 巴多, 旦巴, 鲜莉莉, 旦巴加参, 支张, 平措占堆. 利用微卫星遗传标记评估5个西藏牦牛群体遗传多样性及群体结构[J]. 中国畜牧兽医, 2022, 49(6): 2228-2238.
[4]	郭銮英, 李家磊, 金芹芹, 马骏, 刘全. 牛丙型肝炎病毒研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1994-2000.
[5]	刘晓玮, 李瑞香, 王兴龙, 李晓. 基于mtDNA Cytb基因和D-loop区序列的陕西林麝遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(4): 1352-1363.
[6]	李中波, 侯强红. 湖南省怀化地区白纹伊蚊核糖体18S rRNA和线粒体cox1基因的序列测定及分析[J]. 中国畜牧兽医, 2021, 48(8): 2736-2745.
[7]	房瑞新, 田雪琪, 邹晨, 董依萌, 李洋, 邢秀梅, 刘欣. 利用Y染色体SRY基因分析马鹿的父系起源和遗传多样性[J]. 中国畜牧兽医, 2021, 48(7): 2457-2466.
[8]	李广祯, 马志杰, 陈生梅, 尕布藏, 李瑞哲. 基于mtDNA D-loop序列探究门源白牦牛与天祝白牦牛间的遗传差异[J]. 中国畜牧兽医, 2021, 48(5): 1647-1654.
[9]	肖逸, 王孟文, 张秀英, 古丽扎尔·阿不都克力木, 韩建林, 王玉涛. 基于mtDNA Cytb基因的马可·波罗盘羊遗传多样性与起源进化分析[J]. 中国畜牧兽医, 2021, 48(5): 1681-1690.
[10]	房瑞新, 田雪琪, 邹晨, 董依萌, 刘欣, 邢秀梅. 马鹿的起源进化与遗传多样性研究进展[J]. 中国畜牧兽医, 2021, 48(12): 4575-4587.
[11]	李静, 郭丽君, 王莉, 胡平, 肖逸, 韩建林, 王玉涛. 喀喇昆仑-帕米尔地区牦牛mtDNA D-loop区遗传多样性及系统发育分析[J]. 中国畜牧兽医, 2020, 47(8): 2481-2492.
[12]	李莹, 何国戈, 王艳, 何静怡, 黄爱珍, 郑经成, 葛影影, 罗成龙. 4个优质鸡群体mtDNA D-loop区遗传多样性分析[J]. 中国畜牧兽医, 2020, 47(2): 469-478.
[13]	王坤, 豆腾飞, 李琦华, 柳明正, 李丰耘, 贾俊静, 葛长荣. 云南3个新发掘地方鸡种mtDNA D-loop区序列遗传多样性分析[J]. 中国畜牧兽医, 2020, 47(10): 3232-3241.
[14]	周凡莉, 黄卫平, 金素钰, 杨世忠, 黄林, 饶开晴, 郑玉才. 建昌马mtDNA D-loop区遗传多样性及系统进化分析[J]. 中国畜牧兽医, 2020, 47(1): 63-73.
[15]	李兴才, 潘成勇, 李辉, 杨华婷, 陈林, 肖涛. 香炉山鸡线粒体DNA D-Loop区遗传多样性及系统进化研究[J]. 中国畜牧兽医, 2019, 46(9): 2633-2641.