乳酸菌发酵扶正解毒散复合制剂的制备及其在肉鸡上的应用研究

doi:10.16431/j.cnki.1671-7236.2016.04.032

›› 2016, Vol. 43 ›› Issue (4): 1066-1071.doi: 10.16431/j.cnki.1671-7236.2016.04.032

乳酸菌发酵扶正解毒散复合制剂的制备及其在肉鸡上的应用研究

史洪涛¹, 乔宏兴¹, 王永芬¹, 姜亚乐², 边传周¹

1. 河南牧业经济学院, 郑州 450046;
2. 河南农业大学牧医工程学院, 郑州 450002

收稿日期:2015-09-28 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 边传周 E-mail:chuanzhou-bian@126.com
作者简介:史洪涛(1987-),男,河南安阳人,硕士,初级实验师,研究方向:中药发酵,E-mail:chuangzuo2015@163.com
基金资助:
河南省科技攻关项目(142102310034);河南省科技成果转化项目(142201110029)

Studies on Preparation and Application of Fuzhengjiedusan Compound Preparation Fermentated by Lactic Acid Bacteria in Broilers

SHI Hong-tao¹, QIAO Hong-xing¹, WANG Yong-fen¹, JIANG Ya-le², BIAN Chuan-zhou¹

1. Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2. College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China

Received:2015-09-28 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 本研究旨在制备一种乳酸菌发酵的扶正解毒散复合制剂,并探索其对肉鸡生长性能和免疫器官发育的影响。首先,利用单因子试验法将不同比例的黄芪、板蓝根、淫羊藿及复合中药提取液添加至乳酸菌的培养基中,测定中药对乳酸菌生长的影响;其次,利用正交试验对复合药液培养基进行优化,确定最佳的培养基配方;最后,选取1日龄健康AA肉仔鸡450只,随机分为3组,每组3个重复:对照组(抗生素)、试验Ⅰ组(发酵制剂)、试验Ⅱ组(未发酵制剂),分别在试验的第21、42天测定肉鸡的生长及免疫器官指标。试验结果显示,黄芪、板蓝根、淫羊藿及复合药液组的最高活菌数与对照组相比分别提高了261.64%、505.48%、156.16%、564.38%。通过正交试验得到复合发酵液最适生产培养基配方:复合药液100 mL、红糖2.0 g、蛋白胨0.3 g、豆粕2.0 g,发酵液活菌数为7.13×10⁹ CFU/mL。肉鸡生长试验结果显示,1~42日龄阶段,Ⅰ、Ⅱ组的平均日增重分别比对照组提高了17.84%和8.22% (P<0.05),料肉比分别比对照组降低了10.88%和4.66% (P<0.05),平均日采食量明显高于对照组 (P<0.05);21、42日龄时,Ⅰ、Ⅱ组脾指数分别比对照组提高了11.04%和13.58% (P<0.05)、7.79%和7.41% (P<0.05),法氏囊指数分别比对照组提高了31.30%和26.23% (P<0.05)、15.65%和11.48% (P<0.05)。以上试验结果表明中药提取液能促进乳酸菌的增殖,复合发酵液能够提高肉鸡的生长性能并促进免疫器官的发育。

关键词: 乳酸菌; 复合中药; 发酵; 肉鸡

Abstract: This study was aimed toproduce compound preparations of Fuzhengjiedusan fermented with Lactic acid bacteria and analyze its effectson growth performancesand immune organ growth of broilers. Firstly, to identify the influence of traditional Chinese medicine on the growth of Lactic acid bacteria, the medium for the growth of Lactic acid bacteria was filled with different proportions of Astragalusmembranaceus, Radix Isatidis, Epimedium and compound Chinese herbal medicine extracts by single factor test. Secondly, to confirm the optimum medium, orthogonal test was used to optimize the culture medium of compound medicine liquid. Finally, 450 one-day-old healthy AA broilers were randomized into three groups, each group had 3 repeats: control group (antibiotics), group Ⅰ(fermented preparation), group Ⅱ (unfermented preparation). The growth and immune organ index were determined in 21 and 42 days after administration. The results showed that the highest viable count of Astragalusmembranaceus, Radix Isatidis, Epimedium and compound medicine liquid groups were increased by 261.64%, 505.48%, 156.16% and 564.38%, respectively, compared with control group. The optimum medium composition was compound medicine liquid 100 mL, brown sugar 2.0 g, peptone 0.3 g, soybean meal 2.0 g. The viable count of fermentation liquid was 7.13×10⁹ CFU/mL.The production results showed that in period of 1~42 days, the daily weight gain of group Ⅰ and Ⅱ were separately increased by 17.84% and 8.22% (P<0.05) than that of the control. What's more, the feed conversion rate was reduced by 10.88% (P<0.05). The average daily feed intake was significantly higher than control group (P<0.05). At 21 and 42 days, the spleen index of group Ⅰ were improved by 11.04% and 13.58% (P<0.05), respectively. The bursal index were raised by 31.30% and 26.23% (P<0.05), respectively. However, the spleen index of group Ⅱ were improved by 7.79% and 7.41% (P<0.05), respectively. The bursal index were raised by 15.65% and 11.48% (P<0.05), respectively. On the basis of the above results, it could be suggested that Chinese herbal medicine extracts can promote lactic acid bacteria proliferation and compound fermentation liquid can improve growth performance in broilers, as well as immune organ development.

Key words: lactic acid bacteria; compound Chinese herbal medicine; fermentation; broilers

中图分类号:

S811.6

史洪涛, 乔宏兴, 王永芬, 姜亚乐, 边传周. 乳酸菌发酵扶正解毒散复合制剂的制备及其在肉鸡上的应用研究[J]. , 2016, 43(4): 1066-1071.

SHI Hong-tao, QIAO Hong-xing, WANG Yong-fen, JIANG Ya-le, BIAN Chuan-zhou. Studies on Preparation and Application of Fuzhengjiedusan Compound Preparation Fermentated by Lactic Acid Bacteria in Broilers[J]. , 2016, 43(4): 1066-1071.

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乳酸菌发酵扶正解毒散复合制剂的制备及其在肉鸡上的应用研究

Studies on Preparation and Application of Fuzhengjiedusan Compound Preparation Fermentated by Lactic Acid Bacteria in Broilers

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