一株山羊魏氏梭菌的分离鉴定及其致病性研究

doi:10.16431/j.cnki.1671-7236.2015.07.041

›› 2015, Vol. 42 ›› Issue (7): 1905-1909.doi: 10.16431/j.cnki.1671-7236.2015.07.041

一株山羊魏氏梭菌的分离鉴定及其致病性研究

孟霞¹, 孙翠平¹, 唐娜^1,2, 苗立中^1,2, 沈志强^1,2

1. 山东绿都生物科技有限公司, 滨州 256600;
2. 山东省滨州畜牧兽医研究院, 滨州 256600

收稿日期:2014-10-23 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 沈志强 E-mail:bzshenzq@163.com
作者简介:孟霞(1985-),女,山东滨州人,硕士,研究方向:动物疫病诊断,E-mail:xmeng214@163.com
基金资助:
山东省现代农业产业技术体系羊产业创新团队项目(SATS-201226-3)

Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat

MENG Xia¹, SUN Cui-ping¹, TANG Na^1,2, MIAO Li-zhong^1,2, SHEN Zhi-qiang^1,2

1. Shandong Lvdu Bio-science and Technology Co., Ltd., Binzhou 256600, China;
2. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China

Received:2014-10-23 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 本试验从一例病死山羊组织内分离到一株致病性细菌,通过鉴别培养、生化试验、分子生物学试验和动物致病性试验,证明该病原菌为羊致病性A型魏氏梭菌;毒素基因分析结果显示,该菌同时含有α和β2两种毒素基因;动物致病性试验结果表明,该菌对昆明小鼠具有较强的致病性,并从试验致死的昆明小鼠病料中分离到了与病死山羊病原相一致的细菌,从而确定A型魏氏梭菌为引起该山羊死亡的主要病原菌。本试验结果为该羊场魏氏梭菌病的治疗和预防提供了科学依据。

关键词: 山羊; 魏氏梭菌; 分离; 厌氧培养; 细菌学鉴定

Abstract: A pathogenic bacteria was isolated from a dead goat,and it was identified as goat-pathogenic type A Clostridium perfringens by differential culture,biochemical test,molecular biological technology and animal pathogenicity test.Analysis results of toxin genes showed that this bacteria contained both α and β2 toxin genes.Animal pathogenicity test result showed that this bacteria was highly pathogenic to mice,and the same bacteria was isolated from dead mice as which was isolated from the dead goat,so it was certain that type A Clostridium perfringens was the main pathogenic bacteria which caused the goat dead.The results would provide scientific basis for the treatment and prevention of the disease caused by Clostridium perfringens in this goat farm.

Key words: goat; Clostridium perfringens; isolation; anaerobic cultivation; bacteriological identification

中图分类号:

S852.61⁺6.3

孟霞, 孙翠平, 唐娜, 苗立中, 沈志强. 一株山羊魏氏梭菌的分离鉴定及其致病性研究[J]. , 2015, 42(7): 1905-1909.

MENG Xia, SUN Cui-ping, TANG Na, MIAO Li-zhong, SHEN Zhi-qiang. Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat[J]. , 2015, 42(7): 1905-1909.

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一株山羊魏氏梭菌的分离鉴定及其致病性研究

Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat

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