miR-143在肌细胞中的表达及其影响

›› 2014, Vol. 41 ›› Issue (10): 7-12.

miR-143在肌细胞中的表达及其影响

王辰¹, 张晶¹, 谢炳坤², 周荣¹, 李奎¹, 唐中林¹

1. 中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与种质创新重点实验室, 北京 100193;
2. 广西畜牧研究所, 广西南宁 530001

收稿日期:2014-03-14 出版日期:2014-10-20 发布日期:2014-10-29
通讯作者: 唐中林 E-mail:zhonglinqy_99@sina.com
作者简介:王辰(1988－),男,河北人,硕士,研究方向:动物遗传育种与繁殖。
基金资助:
转基因生物新品种培育重大专项(2013ZX08006-002);中国农业科学院科技创新工程(ASTIP-IAS05);广西家畜遗传改良重点实验室培育基地开放课题(桂牧研科2013GXKFKT-09)

Expression and Function Analysis of miR-143 in Muscle Cells

WANG Chen¹, ZHANG Jing¹, XIE Bing-kun², ZHOU Rong¹, LI Kui¹, TANG Zhong-lin¹

1. The Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Guangxi Institute of Animal Science, Nanning 530001, China

Received:2014-03-14 Online:2014-10-20 Published:2014-10-29

摘要/Abstract

摘要： 本试验旨在研究microRNA-143(miR-143)对小鼠肌细胞增殖和分化的影响。利用Real-time PCR方法对细胞不同分化时期进行检测,并且在小鼠肌细胞C2C12中通过转染miR-143 mimics和Inhibitor对标志基因myogenin(MyoG)进行检测,然后利用免疫荧光和Western blotting方法对组织相容性复合体(major histocompatibility complex,MHC)基因表达水平进行检测。结果表明,miR-143与肌细胞的增殖和分化密切相关,过表达miR-143后,肌细胞标志基因MyoG出现下调,敲低后结果相反,且在分化细胞中过表达miR-143,免疫荧光和Western blotting结果显示,标志基因MHC的表达水平下降。因此,miR-143是一个潜在的影响肌肉生长发育的microRNA,为以后肌肉发育和功能的研究提供了一个新的资料。

关键词: 骨骼肌; C2C12细胞; miR-143; MyoG; 增殖分化

Abstract: This experiment was intended to study effects of microRNA-143 (miR-143) on muscle cell proliferation and differentiation of mice. Using the method of Real-time PCR to detect cell differentiation during the different time,and transfected miR-143 mimics and Inhibitor in C2C12 cells to test the expression of marker gene MyoG,then using the immunofluorescence and Western blotting method to test the MHC gene expression level.The results showed that miR-143 was closely related to the muscle cell proliferation and differentiation. Overexpression of miR-143,we identified that marker gene MyoG display a down-regulated expression,but after transfect siRNA,we found an opposite result. Also overexpression of miR-143 in differentiated cells,the result showed that expression of marker gene MHC was down-regulated. Therefore,miR-143 was a potential gene which had effects on muscle growth and development. For the future research,this study of muscle development and function could provide a new data.

Key words: skeletal muscle; C2C12 cells; miR-143; MyoG; proliferation and differentiation

中图分类号:

王辰, 张晶, 谢炳坤, 周荣, 李奎, 唐中林. miR-143在肌细胞中的表达及其影响[J]. , 2014, 41(10): 7-12.

WANG Chen, ZHANG Jing, XIE Bing-kun, ZHOU Rong, LI Kui, TANG Zhong-lin. Expression and Function Analysis of miR-143 in Muscle Cells[J]. , 2014, 41(10): 7-12.

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