水貂毛皮成熟期皮肤中EGF、IGF-Ⅰ及IGF-ⅠR基因的表达

中国畜牧兽医

水貂毛皮成熟期皮肤中EGF、IGF-Ⅰ及IGF-ⅠR基因的表达

张海华¹，张铁涛¹，宋兴超¹，常忠娟¹，赵靖波¹，周宁¹^，2，李光玉¹

（1.中国农业科学院特产研究所，特种经济动物分子生物学重点实验室，吉林长春 130112；2. 延边大学农学院，吉林延吉 133002）

收稿日期:2014-01-03 出版日期:2014-07-20 发布日期:2014-08-21
通讯作者: 李光玉，研究员，博士生导师。E-mail：tcslgy@126.com
作者简介:张海华（1983—），女，河北人，博士，研究方向：特种经济动物营养与分子营养。
基金资助:
公益性行业（农业）科研专项（200903014）；中国农业科学院特产研究所创新工程专项经费。

Expression of EGF, IGF-Ⅰand IGF-ⅠR Gene in Mink’s Skin and Hair Follicles of Pelting Period

ZHANG Hai-hua¹, ZHANG Tie-tao¹, SONG Xing-chao¹, CHANG Zhong-juan¹, ZHAO Jing-bo¹,ZHOU Ning¹^，2, LI Guang-yu¹

(1. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun 130112, China；2. Agriculture College, Yanbian University, Yanji 133002，China)

Received:2014-01-03 Online:2014-07-20 Published:2014-08-21

摘要/Abstract

摘要： 为探讨水貂皮肤和毛囊发育机理，试验运用组织学和免疫组化方法对水貂毛皮成熟期皮肤及毛囊中表皮生长因子（epidermal growth factor, EGF）和胰岛素样生长因子-Ⅰ（insulin-like growth factor-Ⅰ，IGF-Ⅰ）及其受体（insulin-like growth factor-Ⅰreceptor，IGF-ⅠR）进行了研究。结果表明，水貂皮肤表皮细胞和毛囊外根鞘细胞中都有IGF-Ⅰ、IGF-ⅠR和EGF基因的强阳性表达，3种基因主要分布于细胞质中，细胞核呈空泡状未见到阳性染色，呈苏木精复染的蓝色，皱褶区域和表皮下胶原结缔组织出现了阳性染色为非特异性阳性结果。IGF-Ⅰ、IGF-ⅠR和EGF基因在水貂皮肤表皮和毛囊外根鞘细胞中广泛表达，直接参与调控皮肤和毛囊的发育。表明EGF、IGF-Ⅰ和IGF-ⅠR基因在水貂皮肤及其衍生结构的发育中发挥着重要作用。

关键词: 水貂; 毛囊; 表皮生长因子; 胰岛素样生长因子-Ⅰ; 胰岛素样生长因子-Ⅰ受体

Abstract: In order to study the mechanism of the development of skin and hair follicle of mink, distribution of epidermal growth factor(EGF), insulin-like growth factor Ⅰ(IGF-Ⅰ) and its receptor(IGF-Ⅰ R) in mink’s skin and hair follicles of pelting period were studied with histological methods and immunohistochemistry. The results showed that there was a strong positive expression of IGF-Ⅰ, IGF-ⅠR and EGF gene in mink’s skin epidermal cells and outer root sheath cells, these three genes were mainly distributed in the nucleus of cells, the positive reaction staining was not observed in vacuole, with hematoxylin counterstained blue, collagenous connective tissue and epidermis fold area under the positive staining was positive result of nonspecific. Widespread expression of IGF-Ⅰ, IGF-ⅠR and EGF gene in the epidermis and hair follicle outer root sheath cells of mink skin, directly involved in the regulation of skin and hair follicle development. So EGF, IGF-Ⅰ and IGF-Ⅰ R gene plays an important role in mink skin and its derivatives development.

Key words: mink; hair follicle; EGF; IGF-Ⅰ; IGF-ⅠR

张海华，张铁涛，宋兴超，常忠娟，赵靖波，周宁，李光玉. 水貂毛皮成熟期皮肤中EGF、IGF-Ⅰ及IGF-ⅠR基因的表达[J]. 中国畜牧兽医.

ZHANG Hai-hua, ZHANG Tie-tao, SONG Xing-chao, CHANG Zhong-juan, ZHAO Jing-bo,ZHOU Ning, LI Guang-yu. Expression of EGF, IGF-Ⅰand IGF-ⅠR Gene in Mink’s Skin and Hair Follicles of Pelting Period[J]. China Animal Husbandry & Veterinary Medicine.

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