维生素D3对10～12月龄梅花鹿生长性能、营养物质表观消化率及血清生化指标的影响

doi:10.16431/j.cnki.1671-7236.2024.10.014

摘要/Abstract

摘要： 【目的】探究饲粮维生素D₃(VD₃)添加水平对10～12月龄梅花鹿生长性能、营养物质表观消化率及血清生化指标的影响。【方法】选取24头10月龄、平均体重为(45.32±5.23)kg的健康雄性梅花鹿，随机分成4组，每组6头，每个重复1头，分别饲喂在基础饲粮中添加0(对照组)、2 000、4 000 和8 000 IU/kg VD₃的试验饲粮。试验期共90 d。试验结束后统计梅花鹿体重、采食量，计算生长性能；采集血液样本，测定血清谷胱甘肽过氧化物酶、总超氧化物歧化酶(T-SOD)、碱性磷酸酶水平及丙二醛、免疫球蛋白A、免疫球蛋白G、免疫球蛋白M、甲状腺旁素、降钙素、1,25羟基维生素D₃、钙和磷含量。试验第75天起连续采集3 d粪便样本，测定干物质、粗蛋白质、粗脂肪、酸性洗涤纤维、中性洗涤纤维、钙及磷含量。【结果】 ①与对照组相比，饲粮添加4 000 IU/kg VD₃显著提高了梅花鹿平均日增重(P＜0.05)，其他各组间平均日增重差异不显著(P＞0.05)。②4 000 IU/kg组梅花鹿Ca表观消化率显著高于对照组(P＜0.05)，其他各组间差异不显著(P＞0.05)。③3个试验组梅花鹿血清T-SOD水平均显著高于对照组(P＜0.05)，但其在3组间差异不显著(P＞0.05)；饲粮添加8 000 IU/kg VD₃显著提高了梅花鹿血清1,25羟基维生素D₃水平(P＜0.05)，其他各组间该指标差异不显著(P＞0.05)。【结论】饲粮添加适量VD₃有利于提高梅花鹿生长性能和机体抗氧化能力，增加血清1,25羟基维生素D₃含量。综合试验结果，4 000 IU/kg VD₃在10～12月龄梅花鹿应用效果较好。

关键词: 维生素D₃; 梅花鹿; 生长性能; 血清生化指标

Abstract: 【Objective】 To investigate the effects of vitamin D₃(VD₃) supplementation on growth performance,apparent digestibility of nutrients and serum biochemical indexes of 10-12 months old sika deer (Cervus nippon).【Method】 Twenty-four healthy male sika deers aged 10 months with an average body weight of 45.32 kg±5.23 kg were randomly divided into 4 groups with 6 deers in each group and 1 deer per replicate,which were fed basal diet supplemented with 0 (control group)，2 000，4 000 and 8 000 IU/kg VD₃,respectively. The experiment lasted for 90 days. At the ending of the experiment，the body weight and feed intake of sika deer were counted to determine the growth performance.Blood samples were collected to determine the serum levels of glutathione peroxidase,total superoxide dismutase(T-SOD) and alkaline phosphatase, and the levels of malondialdehyde,immunoglobulin A,immunog-lobulin G,immunoglobulin M,parathyroid，calcitonin，1,25 hydroxyvitamin D₃,calcium and phosphorus. Since the 75th day，fecal samples were collected for 3 days，and the contents of dry matter,crude protein,crude fat,acid detergent fiber,neutral detergent fiber,calcium and phosphorus were determined.【Result】 ①Compared with control group,diet supplemented with 4 000 IU/kg VD₃ significantly increased the average daily gain of sika deer (P＜0.05),but there was no significant difference among other groups (P＞0.05). ②The apparent digestibility of Ca in 4 000 IU/kg group was significantly higher than that in control group (P＜0.05)，but there was no significant difference among other groups (P＞0.05).③The serum T-SOD levels of sika deer in 2 000，4 000 and 8 000 IU/kg groups were significantly higher than that in control group (P＜0.05),but there was no significant difference among 2 000，4 000 and 8 000 IU/kg groups (P＞0.05). Diet supplemented with 8 000 IU/kg VD₃ significantly increased serum 1,25 hydroxyvitamin D₃ level (P＜0.05)，but there was no significant difference among other groups (P＞0.05).【Conclusion】 Dietary VD₃ supplementation was beneficial to improve growth performance，antioxidant capacity and serum 1,25 hydroxyvitamin D₃ content of sika deer. According to the indexes determined in this experiment,4 000 IU/kg VD₃ had a good application effect on 10-12 months old sika deer.

Key words: vitamin D₃; sika deer; growth performance; serum biochemical indexes

中图分类号:

S816.7

张婷, 黄姚, 袁伟涛, 郭肖兰, 王凯英, 徐超. 维生素D₃对10～12月龄梅花鹿生长性能、营养物质表观消化率及血清生化指标的影响[J]. 中国畜牧兽医, 2024, 51(10): 4336-4342.

ZHANG Ting, HUANG Yao, YUAN Weitao, GUO Xiaolan, WANG Kaiying, XU Chao. Effects of Vitamin D₃ on Growth Performance，Apparent Digestibility of Nutrients and Serum Biochemical Indexes of 10-12 Months Old Sika Deer (Cervus nippon)[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4336-4342.

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维生素D₃对10～12月龄梅花鹿生长性能、营养物质表观消化率及血清生化指标的影响

Effects of Vitamin D₃ on Growth Performance，Apparent Digestibility of Nutrients and Serum Biochemical Indexes of 10-12 Months Old Sika Deer (Cervus nippon)

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