不同单宁制剂对南美白对虾生长、血液指标及肠道消化酶活性的影响

doi:10.16431/j.cnki.1671-7236.2023.11.013

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (11): 4444-4453.doi: 10.16431/j.cnki.1671-7236.2023.11.013

不同单宁制剂对南美白对虾生长、血液指标及肠道消化酶活性的影响

彭凯¹, 邱建强^1,2, 徐有良³, 鲁慧杰¹, 赵红霞¹, 陈冰¹, 冼理权⁴, 王玉玺⁵, 黄文¹

1. 广东省农业科学院动物科学研究所, 广东省畜禽育种与营养研究重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省农业科学院水产协同创新中心, 广州 510640;
2. 华中农业大学水产学院, 武汉 430070;
3. 肇庆市佰仕科生物科技有限公司, 肇庆 526105;
4. 肇庆市高要区新桥镇农林水综合服务站, 肇庆 526116;
5. 加拿大农业与农业食品部莱斯布里奇研究中心, 莱斯布里奇 T1J4B1

收稿日期:2023-06-04 出版日期:2023-11-05 发布日期:2023-10-27
通讯作者: 黄文 E-mail:huangwen549@126.com
作者简介:彭凯,E-mail:pengkai1016@126.com。
基金资助:
国家自然科学基金（31902388）；国家外国专家项目（G2022030077L、G2023030042L）；广东省农业科学院协同创新中心课题（XT202301）；广东省乡村振兴战略专项资金种业振兴项目（2022-SPY-00-007）；广东省财政专项资金南美白对虾种质创制与产业化项目（2022SDZG01）；科技创新战略专项资金（高水平农科院建设-青年研究员项目）（R2021PY-QY001）

Effects of Different Plant Tannin Preparations on Growth Performance, Blood Parameters and Intestinal Digestive Enzyme Activities of Litopenaeus vannamei

PENG Kai¹, QIU Jianqiang^1,2, XU Youliang³, LU Huijie¹, ZHAO Hongxia¹, CHEN Bing¹, XIAN Liquan⁴, WANG Yuxi⁵, HUANG Wen¹

1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center of Aquatic Sciences, Guangzhou 510640, China;
2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
3. Zhaoqing Baishike Biotechnology Co., Ltd., Zhaoqing 526105, China;
4. Agriculture, Forestry and Water Comprehensive Service Station of Xinqiao Town in Gaoyao District, Zhaoqing City, Zhaoqing 526116, China;
5. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge T1J4B1, Canada

Received:2023-06-04 Online:2023-11-05 Published:2023-10-27

摘要/Abstract

摘要： 【目的】评价不同植物单宁制剂对南美白对虾（Litopenaeus vannamei）的饲用效果。【方法】选择初始体重为（6.86±0.02） g的南美白对虾600尾，随机分为5组，每组3个重复，每个重复40尾。制备5组等氮等脂饲料，即基础饲料（G1）、含0.1%和0.2%五倍子单宁制剂饲料（G2、G3）、含0.1%和0.2%栗木单宁制剂饲料（G4、G5）。试验周期为42 d。试验结束后统计对虾重量、数量、摄食量，计算生长性能；围心腔采血并分离血清，测定血清生化及血清抗氧化与免疫指标；分离对虾肠道，匀浆后测定消化酶活性。【结果】与G1组相比，G5组对虾饲料系数显著提高（P<0.05），肠体比、出肉率显著下降（P<0.05）；G3组对虾血清甘油三酯及丙二醛含量均显著下降（P<0.05）；G5组对虾血清葡萄糖、甘油三酯、尿素氮含量显著下降（P<0.05），而免疫球蛋白M含量显著提高（P<0.05）；G2、G3组对虾肠道胰蛋白酶活性显著提高（P<0.05）。【结论】以生长性能、抗氧化能力和肠道消化酶活性为综合评价指标，0.2%五倍子单宁制剂对南美白对虾的应用效果较好。

关键词: 水解单宁; 南美白对虾; 生长性能; 抗氧化与免疫

Abstract: 【Objective】 This study was conducted to evaluate the feeding effect of different plant tannin preparations on Litopenaeus vannamei.【Method】 A total of 600 shrimp were randomly selected and divided into 5 groups with 3 replicates per group and 40 shrimp per replicates.Five isonitrogen and isolipid diets were prepared containing 0 (G1), 0.1% (G2) and 0.2% (G3) Chinese gallotannin preparations, and 0.1% (G4) and 0.2% (G5) chestnut tannin preparations.The experiment was lasted for 42 days.At the end of the experiment, the weight, quantity, and feed intake of shrimp each tank were counted to calculate their growth performance.Blood was collected from the cardiocoelom of the sampled shrimp to separate serum for determination of serum biochemical and serum antioxidant and immune indicators, and the intestine of the shrimp was separated for determination of intestinal digestive enzyme activity.【Result】 Compared with G1, the feed coefficient of shrimp in G5 was significantly increased (P<0.05), while the intestinesomatic index and meat rate were significantly decreased (P<0.05).The content of serum triglyceride of shrimp in G3 was significantly decreased (P<0.05), and the contents of serum glucose, triglyceride and urea nitrogen of shrimp in G5 were significantly decreased (P<0.05).Compared with G1, the content of serum malondialdehyde in G3 was significantly decreased (P<0.05), while the content of serum immunoglobulin M in G5 was significantly increased (P<0.05).Compared with G1, intestinal trypsin activity of shrimp in G2 and G3 were significantly increased (P<0.05).【Conclusion】 Taking growth performance, antioxidant capacity and intestinal digestive enzyme activity as comprehensive evaluation indicators, 0.2% Chinese gallotannin preparation had a good application effect on Litopenaeus vannamei.

Key words: hydrolysable tannin; Litopenaeus vannamei; growth performance; antioxidant and immune

中图分类号:

S816.7

彭凯, 邱建强, 徐有良, 鲁慧杰, 赵红霞, 陈冰, 冼理权, 王玉玺, 黄文. 不同单宁制剂对南美白对虾生长、血液指标及肠道消化酶活性的影响[J]. 中国畜牧兽医, 2023, 50(11): 4444-4453.

PENG Kai, QIU Jianqiang, XU Youliang, LU Huijie, ZHAO Hongxia, CHEN Bing, XIAN Liquan, WANG Yuxi, HUANG Wen. Effects of Different Plant Tannin Preparations on Growth Performance, Blood Parameters and Intestinal Digestive Enzyme Activities of Litopenaeus vannamei[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4444-4453.

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不同单宁制剂对南美白对虾生长、血液指标及肠道消化酶活性的影响

Effects of Different Plant Tannin Preparations on Growth Performance, Blood Parameters and Intestinal Digestive Enzyme Activities of Litopenaeus vannamei

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