DNA甲基化在动物遗传育种中的研究进展

摘要/Abstract

摘要： DNA甲基化作为表观遗传学修饰方法之一,对基因的表达发挥重要的调控作用。随着众多DNA甲基化检测技术(如高通量检测技术)的迅速发展对DNA甲基化的生物信息学研究已经变成了一个非常活跃的热点。在动物遗传育种方面,由于DNA甲基化程度的改变,可能影响众多性状相关基因的表达量,因此改变动物的各种性状。所以目前已有相关研究开始探索DNA甲基化在动物遗传育种上的具体作用。

关键词: DNA甲基化; 检测方法; 杂种优势; 遗传育种

Abstract: DNA methylation play an important role in gene expression regulation as one of epigenetic modification of the methods. With the rapid development of the number of DNA methylation detection technology,such as high-throughput detection of DNA methylation,study of DNA methylation bioinformatics has become a very active hot spots. In animal genetics and breeding,traits that may affect the expression of genes can be influenced since the degree of DNA methylation changes,and thus change the characteristics of various animals. Therefore,relevant studies have already begun to explore the specific role of DNA methylation in animal genetics and breeding.

Key words: DNA methylation; detection technology; heterosis; genetics and breeding

中图分类号:

S813.3

邱峥艳, 郭将, 杨春, 赵志辉. DNA甲基化在动物遗传育种中的研究进展[J]. , 2012, 39(7): 173-178.

QIU Zheng-yan, GUO Jiang, YANG Chun, ZHAO Zhi-hui. Research Progress of DNA Methylation in Animal Genetics and Breeding[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(7): 173-178.

参考文献

1 唐韶青,张沅,徐青. 不同动物部分组织基因组甲基化程度的差异分析[J]. 农业生物技术学报,2006,14:507~510.
2 Agrelo R,Setien F,Espada J,et al. Inactivation of the lamin A/C gene by CpG island promoter hypermethylation in hematologic malignancies,and its association with poor survival in nodal diffuse large B-cell lymphoma. J Clin Oncol,2005,23:3940~3947.
3 Aina R,Sgorbati S,Santagostino A,et al. Specific hypomethylation of DNA is induced by heavy metals in white clover and industrial hemp. Physiol Plantarum,2004,121:472~480.
4 Barski A,Zhao K. Genomic location analysis by ChIP-Seq. J Cell Biochem,2009,107:11~18.
5 Bird A P. Gene number,noise reduction and biological complexity. Trends Genet,1995,11:94~100.
6 Clark S J,Harrison J,Frommer M. CpNpG methylation in mammalian cells. Nat Genet,1995,10:20~27.
7 Clark S J,Harrison J,Paul C L,et al. High sensitivity mapping of methylated cytosines. Nucleic Acids Res,1994,22:2990~2997.
8 Colella S,Shen L,Baggerly K A,et al. Sensitive and quantitative universal Pyrosequencing methylation analysis of CpG sites. Biotechniques,2003,35:146~150.
9 de Montera B,Boulanger L,Taourit S,et al. Genetic identity of clones and methods to explore DNA. Cloning Stem Cells, 2004, 6:133~139.
10 Finnegan E J,Brettell R I,Dennis E S. The role of DNA methylation in the regulation of plant gene expression. EXS, 1993, 64:218~261.
11 Hajkova P,Erhardt S,Lane N,et al. Epigenetic reprogramming in mouse primordial germ cells. Mech Dev, 2002, 117:15~23.
12 Hatada I,Hayashizaki Y,Hirotsune S,et al. A genomic scanning method for higher organisms using restriction sites as landmarks. Proc Natl Acad Sci USA, 1991,88:9523~9527.
13 Herman J G,Baylin S B. Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med, 2003, 349:2042~2054.
14 Hoffman B G,Jones S J M. Genome-wide identification of DNA-protein interactions using chromatin immunoprecipitation coupled with flow cell sequencing. J Endocrinol, 2009, 201:1~13.
15 Jaenisch R, Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nature Genetics, 2003, 33:245~254.
16 Jiang C D,Deng C Y, Xiong Y Z. Differences of cytosine methylation in parental lines and F1 hybrid of Large White×Meishan crosses and their effects on F1 performance. Journal of Agricultural Biotechnology,2006,2(3):195~200.
17 Keshet I,Schlesinger Y,Farkash S,et al. Evidence for an instructive mechanism of de novo methylation in cancer cells. Nat Genet,2006,38:149~153.
18 Kouzarides T. Chromatin modifications and their function. Cell,2007,128:693~705.
19 Labra M,Grassi F,Imazio S,et al. Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L. Chemosphere, 2004, 54:1049~1058.
20 Lewin J,Schmitt A O,Adorjan P,et al. Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates. Bioinformatics, 2004, 20:3005~3012.
21 Li E,Bestor T H,Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell, 1992, 69:915~926.
22 Li X Q,Xu M L,Korban S S. DNA methylation profiles differ between field- and in vitro-grown leaves of apple. J Plant Physiol, 2002, 159:1229~1234.
23 Lister R,Ecker J R. Finding the fifth base: genome-wide sequencing of cytosine methylation. Genome Res, 2009, 19:959~966.
24 Madlung A,Masuelli R W,Watson B,et al. Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiol, 2002, 129:733~746.
25 Mayer W,Niveleau A,Walter J,et al. Demethylation of the zygotic paternal genome. Nature, 2000, 403:501~502.
26 Neff T,Armstrong S A. Chromatin maps,histone modifications and leukemia. Leukemia, 2009, 23:1243~1251.
27 Okazaki Y,Okuizumi H,Sasaki N,et al. An expanded system of restriction landmark genomic scanning (RLGS Ver. 1.8). Electrophoresis, 1995, 16:197~202.
28 Rabinowicz P D,Palmer L E,May B P,et al. Genes and transposons are differentially methylated in plants,but not in mammals. Genome Res, 2003, 13:2658~2664.
29 Rakyan V K,Hildmann T,Novik K L,et al. DNA methylation profiling of the human major histocompatibility complex: A pilot study for the human epigenome project. Plos Biol, 2004, 2:2170~2182.
30 Ramsahoye B H,Biniszkiewicz D,Lyko F,et al. Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a. P Natl Acad Sci USA, 2000, 97:5237~5242.
31 Raval A,Lucas D M,Matkovic J J,et al. TWIST2 demonstrates differential methylation in immunoglobulin variable heavy chain mutated and unmutated chronic lymphocytic leukemia. J Clin Oncol, 2005, 23:3877~3885.
32 Ronaghi M,Uhlen M,Nyren P. A sequencing method based on real-time pyrophosphate. Science, 1998, 281:363~365.
33 Sato S,Yoshimizu T,Sato E,et al. Erasure of methylation imprinting of Igf2r during mouse primordial germ-cell development. Mol Reprod Dev, 2003, 65:41~50.
34 Shaked H,Kashkush K,Ozkan H,et al. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell, 2001, 13:1749~1759.
35 Slotkin R K,Martienssen R. Transposable elements and the epigenetic regulation of the genome. Nat Rev Genet, 2007, 8:272~285.
36 Song F,Smith J F,Kimura M T,et al. Association of tissue-specific differentially methylated regions (TDMs) with differential gene expression. Proc Natl Acad Sci USA, 2005, 102:3336~3341.
37 Takai D,Jones P A. Comprehensive analysis of CpG islands in human chromosomes 21 and 22. Proc Natl Acad Sci USA, 2002, 99:3740~3745.
38 Tost J,Dunker J,Gut I G. Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing. Biotechniques, 2003, 35:152~156.
39 Wang Y,Leung F C C. An evaluation of new criteria for CpG islands in the human genome as gene markers. Bioinformatics, 2004, 20:1170~1177.
40 Weber M,Davies J J,Wittig D,et al. Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells. Nat Genet, 2005, 37:853~862.
41 Xiong L Z,Xu C G,Maroof M A S,et al. Patterns of cytosine methylation in an elite rice hybrid and its parental lines,detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet, 1999, 261:439~446.
42 Xu M L,Li X Q, Korban S S. AFLP-based detection of DNA methylation. Plant Mol Biol Rep, 2000, 18:361~368.
43 Xu Q,Sun D X,Zhang Y. F-MSAP:A practical system to detect methylation in chicken genome. Chinese Science Bulletin, 2005, 18:2039~2044.
44 Xu Q,Zhang Y,Sun D X,et al. Analysis on DNA methylation of various tissues in chicken. Anim Biotechnol, 2007, 18:231~241.
45 Yan P S,Chen C M,Shi H D,et al. Dissecting complex epigenetic alterations in breast cancer using CpG island microarrays. Cancer Res, 2001, 61:8375~8380.
46 Yang C,Zhang M J,Niu W P,et al. Analysis of DNA methylation in various swine tissues. Plos One, 2011, 6:47.

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