饲喂苜蓿鲜草和全价颗粒饲料对兔胃抗氧化性能和肌层结构的影响

doi:10.16431/j.cnki.1671-7236.2023.01.015

摘要/Abstract

摘要： 【目的】通过比较饲喂苜蓿鲜草和全价颗粒饲料对兔胃黏膜抗氧化能力和胃肌层结构的影响，为兔健康饲养和提高养殖户的收益提供依据。【方法】选用20只健康、体重相近的断奶新西兰兔，随机分为饲料组和苜蓿草组，每组10只，分别饲喂苜蓿鲜草和全价颗粒饲料，饲养30 d。通过空气栓塞法将新西兰兔处死后解剖，称量胃的长度和重量，利用生物化学法测定胃黏膜的抗氧化能力，包括总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和脂质过氧化物（LPO）、丙二醛（MDA）含量，胃pH以及胃蛋白酶的活性，同时运用组织切片技术、苏木精伊红染色及图像分析法测定胃的贲门部、胃底部、胃体部以及幽门部的肌层厚度。【结果】饲料组体重和胃液pH均显著高于苜蓿草组（P<0.05），而苜蓿草组胃相对重量、胃蛋白酶浓度以及胃黏膜抗氧化能力均显著或极显著高于饲料组（P<0.05；P<0.01）。苜蓿草组兔胃贲门部、胃底部以及幽门部的总肌层厚度均极显著大于饲料组（P<0.01），在贲门部苜蓿草组的纵行肌厚度显著大于饲料组（P<0.05），环行肌厚度极显著大于饲料组（P<0.01），在胃底部苜蓿草组斜行肌厚度显著大于饲料组（P<0.05），而其余部位的肌层厚度均无显著差异（P>0.05）。【结论】不同饲喂条件下，苜蓿草组胃化学消化能力、抗氧化能力以及胃肌层厚度均显著高于饲料组。

关键词: 新西兰兔; 食草; 食饲料; 胃壁肌层; 抗氧化

Abstract: 【Objective】 The aim of this study was to compare the effect of feeding fresh alfalfa and full-price pellets on the antioxidant capacity of gastric mucosa and the structure of gastric muscle layer in rabbits, and provide basis for healthy feeding of rabbits and improving the income of farmers.【Method】 Twenty healthy weaned New Zealand rabbits of similar weight were randomly divided into two groups:Alfalfa and feed groups, with ten rabbits in each group.The rabbits in the two groups were fed with fresh alfalfa and full price pellet feed for 30 days, respectively.New Zealand rabbits were sacrificed by air embolization and dissected, and the length and weight of stomachs were measured.The antioxidant capacity of gastric mucosa, including the activity of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and the contents of lipid peroxidation (LPO) and malondialdehyde (MDA), and the gastric pH and pepsin activity were determined by biochemical methods.At the same time, the histological section technique, hematoxylin and eosin staining and image analysis were used to determine the thickness of muscle layer of gastric cardia, fundus, corpus and pylorus.【Result】 The body weight and gastric juice pH of feed group were significantly higher than that of alfalfa group (P<0.05), while the relative gastric weight, the concentration of pepsin and the antioxidant capacity of gastric mucosa in alfalfa group were significantly or extremely significantly higher than those in feed group (P<0.05 or P<0.01).The thickness of total muscle layer in the gastric cardia, fundus and pylorus of alfalfa group were significantly greater than that of feed group (P<0.01).In the cardia, the thickness of longitudinal muscle in alfalfa group was significantly larger than that in feed group (P<0.05), and the thickness of circular muscle in alfalfa group was extremely significantly larger than that in feed group (P<0.01).The thickness of the oblique muscle at the bottom of stomach in alfalfa group was significantly larger than that in feed group (P<0.05).There was no significant difference in the thickness of muscle layer in other parts (P>0.05).【Conclusion】 The gastric chemical digestibility, antioxidant capacity and gastric muscle layer thickness of alfalfa group were significantly higher than those of feed group under different feeding conditions.

Key words: New Zealand rabbit; herbivority; feed; gastric wall muscular; antioxidant

中图分类号:

S829.1

仲华玲, 何丛丛, 吴邦元. 饲喂苜蓿鲜草和全价颗粒饲料对兔胃抗氧化性能和肌层结构的影响[J]. 中国畜牧兽医, 2023, 50(1): 143-150.

ZHONG Hualing, HE Congcong, WU Bangyuan. Effects of Fresh Alfalfa and Full-price Pellet Feed on the Antioxidant Properties and Muscle Structure of Rabbit Stomach[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 143-150.

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