中药牛皮消多糖体外抗新城疫病毒作用的研究

doi:10.16431/j.cnki.1671-7236.2019.09.029

摘要/Abstract

摘要： 为探讨牛皮消多糖（CAP）对体外抗新城疫病毒（NDV）能力的影响，本试验用水提醇沉法提取牛皮消多糖，通过苯酚硫酸法和红外光谱对牛皮消多糖进行检测，采用MTT法测定牛皮消多糖对鸡胚成纤维细胞（CEF）的安全浓度，多糖的安全浓度统一为625 μg/mL，选择安全浓度范围内的（39.06~625 μg/mL）5种浓度的各级多糖，用3种加药方式（先加多糖后接种病毒、先接种病毒后加多糖、多糖和病毒感作后加药）检测牛皮消多糖对NDV的阻断、抑制及直接杀灭作用。结果显示，先接病毒后加多糖组和多糖与病毒感作后加药组CAP₅₀、CAP₃₀、CAP_t、CAP₈₀和CAP₇₀均能显著抑制NDV感染CEF的能力，两组各级多糖抑制率分别为131.23%、110.12%、108.15%、100.24%、93.07%和61.76%、43.73%、44.51%、29.02%、37.45%；先加多糖后接病毒组CAP₅₀、CAP₃₀能显著抑制NDV感染CEF的作用（P<0.05），阻断作用下对NDV的抑制率可达到15.82%、8.33%，其余各组在安全浓度范围内对NDV无抑制作用。各组中药牛皮消多糖均有较好的抗NDV活性，其中对NDV的抑制作用和直接杀灭作用强于对其阻断作用。CAP₅₀及CAP₃₀的抗NDV活性较强，可作为进一步研究材料并具备一定的研究价值。

关键词: 牛皮消多糖; 鸡胚成纤维细胞; 新城疫病毒; 抗病毒

Abstract: In order to investigate the effect of Cynanchum auriculatum polysaccharide (CAP) on the resistance to Newcastle disease virus (NDV) in vitro,the polysaccharides from Cynanchum auriculatum was extracted by water and alcohol precipitation,phenol sulfuric acid and infrared spectroscopy were used to detect CAP.The safe concentration of CAP on chicken embryo fibroblast (CEF) was determined by MTT method,the safe concentration of CAP was set up to 625 μg/mL,and 5 concentrations in the safe concentration range (39.06 to 625 μg/mL) were selected.Each CAP and NDV were added with three kinds of dosing methods (add CAP and then inoculate the virus,inoculate the virus and then add CAP,CAP and the virus-sensing) to detect the blocking,inhibition and direct effect of CAP on NDV.The results showed that CAP₅₀,CAP₃₀,CAP_t,CAP₈₀ and CAP₇₀ could significantly inhibit the ability of NDV to infect CEF after the virus was first added group and the CAP and the virus-sensing group.The inhibition rates of CAP₅₀,CAP₃₀,CAP_t,CAP₈₀ and CAP₇₀ were 131.23%,110.12%,108.15%,100.24%,93.07% and 61.76%;43.73%,44.51%,29.02% and 37.45%,respectively.The addition of CAP₅₀ and CAP₃₀ in CAP followed by the virus group could significantly inhibited NDV (P<0.05) with 15.82% and 8.33% inhibition rate under the blocking condition,and the other groups had no inhibitory effect on NDV within the safe concentration range.In conclusion,the CAP had better anti-NDV activity,and the inhibition and direct killing effect on NDV was stronger than the blocking effect.CAP₅₀ and CAP₃₀ had stronger anti-NDV activity and could be used as research materials to study further.

Key words: Cynanchum auriculatum polysaccharide; chicken embryo fibroblast (CEF); Newcastle disease virus (NDV); antivirus

中图分类号:

S854.5

布合丽倩穆·依明, 赛福丁·阿不拉, 艾克拜尔·依明, 胡安古丽·努尔别克, 阿克珠利·努尔包, 李昊. 中药牛皮消多糖体外抗新城疫病毒作用的研究[J]. 中国畜牧兽医, 2019, 46(9): 2737-2744.

BUHELIQIANMU·Yiming, SAIFUDING·Abula, AIKEBAIER·Yiming, HUANGULI·Nuerbieke, AKEZHULI·Nuerbao, LI Hao. Study on Antiviral Effect of Polysaccharides from Chinese Medicine Cynanchum auriculatum on Newcastle Disease Virus in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2737-2744.

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