›› 2009, Vol. 36 ›› Issue (12): 89-92.

• 遗传繁育 • 上一篇    下一篇

六盘山肉牛杂交改良群体Pit-1基因多态性分析

李聚才1, 张春珍2, 杨易2, 于建勇3, 杨治齐3, 唐国崇3, 杨麦堆3   

  1. (1.宁夏农林科学院草畜工程技术研究中心, 银川 750002; 2.宁夏大学, 银川 750021; 3.泾源县农牧与科技局, 泾源 756400)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-12-20 发布日期:2009-12-20

Study on Polymorphisms of Pit-1 Gene in Improved Hybrid BeefCattle Groups from Liupan Mountain Area

LI Ju-cai1, ZHANG Chun-zhen2, YANG Yi2, YU Jian-yong3, YANG Zhi-qi3, TANG Guo-chong3, YANG Mai-dui3   

  1. (1.Research Center of Grass and Livestock of Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002, China;2.Ningxia University, Yinchuan 750021, China;3.Office of Agro-farming and Science-Technology of Jingyuan County, Jingyuan 756400, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20

摘要: 本试验旨在对宁夏六盘山区肉牛杂交改良群体垂体特异性转录因子1(Pit-1)基因的多态性进行研究,为其杂交改良提供理论基础。采用聚合酶链式反应—限制性片段长度多态性方法(PCR-RFLP),对该群体101个个体Pit-1基因多态性进行检测。结果表明,扩增出的451 bp片段为目的基因片段,其PCR产物经限制性内切酶HinfⅠ消化后表现出多态性,共检测到AA、BB和AB 3种基因型。其中AA基因型频率6.93%(7个);AB基因型频率34.65%(35个);BB型基因型频率58.42%(59个),等位基因A频率24.26%;等位基因B频率75.74%。因此,宁夏六盘山地区的肉牛杂交改良群体Pit-1基因具有多态性。

关键词: 肉牛; Pit-1; PCR-RFLP

Abstract: Polymorphism of the pituitary special transcription factor-1(Pit-1) in improved hybrid beef cattle group from Liupan mountain area in Ningxia province was investigated to give the rationale for hybrid improvement. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) technology was carried out to examine polymorphisms of Pit-1 gene of 101 individuals.The results showed that PCR products of 451 bp digested by restriction enzyme Hinf I showed polymorphisms. The frequencies of three genotypes (AA, AB and BB) were 6.93%(7 individuals),34.65%(35 individuals) and 58.42%(59 individuals).There were three genotypes of Pit-1 gene in improved hybrid beef cattle group from Liupan mountain area in Ningxia province. 

Key words: beef cattle; pituitary special transcription factor 1; PCR-RFL

中图分类号: