2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.02.021

摘要/Abstract

摘要：

试验探讨了2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析。选择120头12月龄、体重250 kg左右的健康西门塔尔杂种肉牛进行运输应激试验，并根据牛基因组遗传图谱，选择23号染色体上与HSP70基因连锁的微卫星座位BMS468和BM1258检测其在西门塔尔杂种肉牛样本群体中的多态性，采用最小二乘拟合一般线性模型分析微卫星座位与运输应激性状部分指标之间的关联效应。结果显示：微卫星座位BMS468共检测到5个等位基因（128、134、140、146、154 bp），优势等位基因为128和134 bp，有效等位基因数（Ne）、多态信息含量（PIC）和遗传杂合度（He）分别为3.66、0.68和0.73，遗传多态性较高，关联分析显示与运输后7 d平均日增重和发病率显著相关，其中128/128 bp基因型个体的运输后7 d平均日增重最大（P < 0.05），134/128 bp基因型个体的发病率最低（P < 0.05）；微卫星座位BM1258座位共检测到6个等位基因（99、101、103、113、117、119 bp），优势基因为101 bp，Ne、PIC和He分别为4.30、0.73和0.77，遗传多态性较丰富，关联分析显示，其与发病率显著相关，其中99/99 bp基因型个体的发病率最低（P < 0.05）。综上所述，2个微卫星座位可作为牛运输应激性状的潜在遗传标记，为开展牛抗运输应激性状的标记辅助选择提供科学依据。

关键词: 肉牛; 运输应激; 微卫星座位; 遗传标记

Abstract:

The correlation between 2 microsatellite loci linked to the HSP70 gene and transport stress traits in beef cattle was analyzed in this study.120 12-month old health Simmental crossbred beef cattle with 250 kg average body weight were used in transport stress test. Two microsatellite loci (BMS468 and BM1258) linked to HSP70 gene mapped to chromosome 23 were selected to study genetic polymorphisms in Simmental crossbred beef cattle population, the relation between microsatellite polymorphisms and transport stress traits were also analyzed by least square fitting general linear model. The results showed that:In BMS468 microsatellite locus,five alleles were detected (128,134,140,146 and 154 bp), the dominant allele was 128 and 134 bp,the effective allele number (Ne),polymorphism information content (PIC) and genetic heterozygosity (He) were 3.66,0.68 and 0.73,respectively.The correlation analysis showed that BMS468 microsatellite locus had a significant effect on the 7 d (after transport) average daily gain and disease incidence,the 7 d (after transport) average daily gain of 128/128 bp genotype was the highest (P < 0.05),and the disease incidence of 134/128 bp genotype was the lowest (P < 0.05).In BM1258 microsatellite locus, six alleles were detected (99,101,103,113,117 and 119 bp), the dominant allele was 101 bp,the Ne,PIC and He were 4.30,0.73 and 0.77,respectively. The correlation analysis showed that BM1258 microsatellite locus had a significant effect on the disease incidence (P < 0.05),and the disease incidence of 99/99 bp genotype was the lowest (P < 0.05).In conclusion,the 2 microsatellite loci with a rich genetic polymorphisms were valuable potential genetic markers for marker-assisted selection of anti-transport stress traits in beef cattle,providing the scientific basis for the markers selection for the anti-transport stress characteristics of cattle.

Key words: beef cattle; transport stress; microsatellite loci; genetic marker

中图分类号:

S813.3

刘延鑫, 孙宇, 李业亮, 翟磊, 郝万清, 高腾云. 2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析[J]. 《中国畜牧兽医》, 2018, 45(2): 456-462.

LIU Yanxin, SUN Yu, LI Yeliang, ZHAI Lei, HAO Wanqing, GAO Tengyun. Correlation Analysis Between 2 Microsatellite Loci Linked to the HSP70 Gene and Transport Stress Traits in Beef Cattle[J]. , 2018, 45(2): 456-462.

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