陇东肉牛PON1基因结构与功能的生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.07.003

摘要/Abstract

摘要：

为阐明陇东肉牛对氧磷酶1（paraoxonase 1，PON1）基因的结构与功能，本研究运用PCR扩增陇东肉牛PON1基因所有外显子序列并测序，通过Seqman软件完成序列拼接，结合生物信息学方法预测和分析其碱基组成、开放阅读框及编码蛋白的理化性质、原子组成、氨基酸残基数、亲水性和疏水性、分子质量、等电点、二级结构、高级结构等。结果显示，陇东肉牛PON1基因CDS区序列全长1 068 bp，编码355个氨基酸残基，其中数目最多的为亮氨酸（Leu），数目最少的为半胱氨酸和色氨酸（Cys和Trp）。碱基组成中A+T含量（56.55%）高于G+C含量（43.26%）。与GenBank中牛PON1基因序列（登录号：EU289337）比对分析发现，在PON1基因外显子4、6、8、9中存在突变，但均未导致氨基酸发生变化，为同义突变。PON1蛋白的原子组成是C₁₈₁₀H₂₇₉₆N₄₅₄O₅₃₃S₁₂，分子质量为39.83 ku，理论等电点为5.24，为稳定的水溶性蛋白质。PON1蛋白二级结构中无规则卷曲、延展链、α-螺旋和β-转角分别由159、116、55和25个氨基酸构成，最终形成了以无规则卷曲和延展链为主的混合型。PON1蛋白的疏水性结果表明，第8位苏氨酸（Thr）疏水性最强（最高分值2.878），第299位脯氨酸（Pro）亲水性最强（最低分值-2.222）。本试验结果为深入研究陇东肉牛PON1蛋白功能及探讨PON1基因突变对甘肃地方肉牛胴体、肉质性状的影响奠定基础。

关键词: 陇东肉牛; PON1基因; 生物信息学; 突变

Abstract:

In order to clarify the structure and function of paraoxonase 1 (PON1) gene,all exon sequences of PON1 gene in Longdong cattle were amplified by PCR method,the complete sequence was assembled by Seqman software,and the base composition,open reading frame,physical and chemical properties of coding protein,atomic composition,number of amino acid residues,hydrophilicity and hydrophobicity,molecular weight,isoelectric point,secondary structure,higher structure,etc were predicted and analyzed by bioinformatics method.The results showed that the CDS sequence of PON1 gene in Longdong cattle was 1 068 bp,encoding 355 amino acids,Leu was the most abundant,Cys and Trp were the least.The content of A+T (56.55%) was higher than that of G+C (43.26%) in base composition.Compared with the published sequence (GenBank accession No.:EU289337) of cattle,we found that there were mutations which were a synonymous mutation in exons 4,6,8 and 9,but none of them changed amino acids.PON1 protein was a stable water-soluble protein,the atomic composition was C₁₈₁₀H₂₇₉₆N₄₅₄O₅₃₃S₁₂,the molecular mass was 39.83 ku,and the theoretical isoelectric point was 5.24.The number of amino acids of random coil,extended strand,α-helix and β-turn in the secondary structure of PON1 protein was 159,116,55 and 25,respectively,eventually formed a hybrid model with random coil and extended strand.Hydrophobic results of PON1 protein showed that the 8th Thr had the strongest hydrophobicity (the highest score:2.878) and the 299th Pro at the highest hydrophilicity (the lowest score:-2.222).The results of this study would lay a foundation for further study on the function of PON1 protein in Longdong cattle and explore the effect of PON1 gene mutation on the carcass and meat quality traits of local cattle in Gansu province.

Key words: Longdong cattle; PON1 gene; bioinformatics; mutation

中图分类号:

张娟, 母童, 赵平, 冯小芳, 吴建平, 顾亚玲. 陇东肉牛PON1基因结构与功能的生物信息学分析[J]. 《中国畜牧兽医》, 2018, 45(7): 1750-1758.

ZHANG Juan, MU Tong, ZHAO Ping, FENG Xiaofang, WU Jianping, GU Yaling. Bioinformatics Analysis of Structure and Function of PON1 Gene in Longdong Cattle[J]. , 2018, 45(7): 1750-1758.

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