›› 2012, Vol. 39 ›› Issue (1): 142-145.

• 遗传繁育 • 上一篇    下一篇

长顺绿壳蛋鸡雌激素受体1基因SNP与蛋品质性状的相关性研究

韦雄1, 邹成松1, 张勇1, 李丹超1, 王现科1, 孙娟2, 杨忠诚2, 陈祥1   

  1. 1. 贵州大学动物科学学院,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳 550025;2. 贵州省畜禽品种改良站,贵州贵阳 550001
  • 收稿日期:2011-06-14 修回日期:1900-01-01 出版日期:2012-01-20 发布日期:2012-01-20
  • 通讯作者: 陈祥

Study on Association of ESR1 Gene with Egg Quality of Changshun Blue-shell Chickens

WEI Xiong1, ZOU Cheng-song1, ZHANG Yong1, LI Dan-chao1, WANG Xian-ke1, SUN Juan2, YANG Zhong-cheng2, CHEN Xiang1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Sciences, Guizhou University,Guiyang 550025,China;2. Guizhou Provincial Livestock Bureau,Guiyang 550001,China
  • Received:2011-06-14 Revised:1900-01-01 Online:2012-01-20 Published:2012-01-20

摘要: 为加快贵州长顺绿壳蛋鸡选育与开发利用,采用PCR-SSCP和DNA测序技术对124只长顺绿壳蛋鸡的雌激素受体1(estrogen receptor,ESR1)基因进行多态性分析,并与其蛋品质性状进行关联性研究。结果表明,长顺绿壳蛋鸡ESR1基因中出现AA、AC和CC 3种基因型,其由于A(-999)C 1个SNPs所导致,经χ2适合性检验,基因型分布偏离了Hardy-Weinberg平衡(P<0.05)。基因型与蛋品质性状关联性分析结果显示,3种基因型个体间的蛋重、蛋形指数、蛋黄比率、蛋白比率、蛋壳重差异不显著(P>0.05),而AA与AC基因型个体的蛋壳厚度差异显著(P<0.05),在不影响其它性状的情况下,选择AA基因型个体可得到良好的蛋壳厚度,从而增强蛋品质。

关键词: 绿壳蛋鸡; ESR1; SNP; 蛋品质

Abstract: In order to quicken breeding and exploitation of Guizhou Changshun Blue-shell chickens, PCR-SSCP and DNA sequence technology were used to analyze polymorphisms and association of estrogen receptor 1(ESR1) gene polymorphisms with egg quality from 124 Changshun Blue-shell chickens. The results showed that there were three kinds of genotypes(AA,AC and CC) in ESR1 gene resulted from A(-999)C, the distribution of genotypes deviated from Hardy-Weinberg equilibrium(P<0.05) by Chi square test,the difference among egg weight,egg shape index,egg yolk and albumen ratio,eggshell weight was not significant(P>0.05),whereas the difference of eggshell thickness between AA and AC genetypes was significant individuals of AA genotype could get better eggshell thickness, which might strengthen egg quality.

Key words: Blue-shell chickens; estrogen receptor(ESR1); SNP; egg quality

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