›› 2011, Vol. 38 ›› Issue (6): 126-128.

• 遗传繁育 • 上一篇    下一篇

百宜黑鸡与兴义矮脚鸡线粒体DNA 控制区遗传多样性分析

王文涛1, 陈彬1, 阳光远2, 傅筑荫1   

  1. 1. 贵州大学,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳 550025;2. 贵州省现代农业发展研究中心,贵州贵阳 550006
  • 收稿日期:2010-12-16 修回日期:1900-01-01 出版日期:2011-06-20 发布日期:2011-06-20
  • 通讯作者: 陈彬

Genetic Diversity in Baiyi Black Chicken and Xingyi Bantam by Analyzing the mtDNA Control Region

WANG Wen-tao1, CHEN Bin1, YANG Guang-yuan2, FU Zhu-yin1   

  1. 1. Key Laboratory of Animal Genetic, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education,Guizhou University, Guiyang 550025,China;2. Guizhou Institute of Modern Agricultural Development,Guiyang 550006,China
  • Received:2010-12-16 Revised:1900-01-01 Online:2011-06-20 Published:2011-06-20

摘要: 采用PCR和直接测序的方法测定百宜黑鸡和兴义矮脚鸡线粒体DNA控制区全序列,比较分析两种贵州地方鸡种的遗传变异。结果表明,百宜黑鸡、兴义矮脚鸡mtDNA控制区全序列长均为1231 bp;共发现26个变异位点(不包含种内变异位点),占分析位点总数的2.11%,其中12个转换,14个颠换;百宜黑鸡mtDNA控制区的A、T、G、C碱基含量分别为27.096%、33.628%、13.117%、26.159%,矮脚鸡的A、T、G、C碱基含量分别是A26.916%、T33.409%、G13.387%、C26.288%,t检验结果显示,两种鸡mtDNA控制区的A和T含量差异显著。百宜黑鸡和兴义矮脚鸡的遗传距离是0.0379,根据分子钟计算,二者约在190万年前分歧进化。

关键词: 百宜黑鸡; 兴义矮脚鸡; 线粒体DNA控制区; 遗传多样性

Abstract: PCR and direct sequencing methods were used to analyze the entire mtDNA D-loop in Baiyi Black chicken and Xingyi Bantam. The D-loop of Baiyi Black chicken and Xingyi Bantam was 1231 bp,there were 26 variational sites, including 12 transitions and 14 transversions. The nucleotide composition of Baiyi Black chicken was A 27.096%, T 33.628%, G 13.117%, C 26.159%, and A 26.916%, T 33.409%, G 13.387%, C 26.288% of Xingyi Bantam. The genetic distance between two partridges was 0.0379. According to the calibrated rates of molecular evolution, they speciated about 1.9 million years ago.

Key words: Baiyi Black chicken; Xingyi Bantam; mtDNA D-loop; genetic diversity

中图分类号: