›› 2009, Vol. 36 ›› Issue (11): 63-65.

• 生物技术 • 上一篇    下一篇

动物体内微生物的宏基因组学分析

李珊珊,王加启,魏宏阳,杨永新,刘开朗,赵圣国,卜登攀   

  1. (中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室, 北京 100193)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-20 发布日期:2009-11-20
  • 通讯作者: 王加启

Metagenomic Analysis of the Microorganisms in Animals

LI Shan-shan, WANG Jia-qi, WEI Hong-yang, YANG Yong-xin, LIU Kai-lang, ZHAO Sheng-guo, BU Deng-pan   

  1. (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
  • Contact: WANG Jia-qi

摘要: 随着分子生物学技术的快速发展及其在微生物研究中的广泛应用,宏基因组学分析方法得到了快速发展。宏基因组学通过直接从动物体内的微生物中提取总DNA,构建宏基因组文库,利用基因组学的研究方法研究样品所包含的全部微生物的遗传组成及其群落功能。作者综述了一些研究基因组组成和动物体内细菌物种的数据库和试验技术,以及肠道微生物的宏基因组学分析。

关键词: 微生物; 宏基因组学; 文库构建

Abstract: With the development of molecular biology and its application in microbiology,a emerging field of metagenomics has been developed rapidly. Metagenomics,comprising extracting total community DNA,constructing genomic library,and analyzing library with similar strategies for functional genomics,provides a powerful tool to study the microorganisms in the complex habitats. In this context,we will review some of the databases and technologies that are used in the study of genome composition of individual strains or species of animal bacteria,as well as the metagenomic analysis of gastrointestinal microorganisms.

Key words: microorganism; metagenomics; library constructing

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