新型环境消毒剂全光谱二氧化钛的消毒效果评价

doi:10.16431/j.cnki.1671-7236.2025.05.047

摘要/Abstract

摘要： 【目的】对新型全光谱二氧化钛消毒剂进行消毒效果评价，为该消毒剂的临床应用提供理论基础和参考依据。【方法】通过实验室消毒试验，首先考察全光谱二氧化钛消毒剂消毒效果，在消毒剂喷涂作用24 h后涂布不同菌液(金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌和大肠杆菌)并培养，观察菌株生长状况，评估该消毒剂对常见细菌的预防效果。在培养基上涂布菌液后立即喷涂消毒剂，在不同时间段采样培养并计算杀菌率，考察该消毒剂对细菌的杀灭效果。将金属腐蚀试片浸泡于消毒剂中，评估其对临床生产设备的侵蚀程度。同时考察了光照对消毒剂抑菌效果的影响。另在规模化养殖场开展临床消毒试验，在使用消毒剂前后分别对栋舍内空气及物体表面进行采样并培养，通过统计菌落总数考察消毒剂在临床应用中的杀菌效果，从而全面评价该消毒剂的消毒效果和安全性。【结果】在实验室消毒评价中，预防试验结果显示，受试消毒剂组除铜绿假单胞菌外均无细菌定植。杀菌试验结果显示，受试消毒剂在10 min即可发挥较好灭菌效果，对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的杀菌率在99.80%以上；6 h后对铜绿假单胞菌的杀菌率下降。金属腐蚀性试验结果显示，受试消毒剂原液对304不锈钢作用72 h的腐蚀速率为0.0047 mm/a，基本无腐蚀性。光照试验结果显示，避光组与非避光组均无细菌定植。在临床生产考察中，与消毒剂对照组相比，受试消毒剂组猪舍空气菌落总数极显著降低(P＜0.01)，物体表面菌落总数无显著变化(P＞0.05)。【结论】全光谱二氧化钛消毒剂对常见致病菌有较强的杀灭作用，在临床效果评价中表现良好，对空气中细菌的杀菌率高达100%，且使用成本较低，拥有较好的应用发展前景，为规模化养殖场的环境消毒提供了一种新的方案。

关键词: 二氧化钛; 环境消毒; 杀菌; 抑菌

Abstract: 【Objective】 The purpose of this experiment was to evaluate the disinfection effect of the new full spectrum TiO₂ disinfectant and provide theoretical basis and reference for the clinical application of this disinfectant.【Method】 Through the laboratory disinfection test,the disinfection effect of full spectrum TiO₂ disinfectant was first investigated,and different bacterial liquids (Staphylococcus aureus,Bacillus subtilis,Pseudomonas aeruginosa and Escherichia coli) were coated and cultured for 24 h after spraying the disinfectant,and the growth status of the strains was observed to evaluate the preventive effect of the disinfectant on common bacteria.The disinfectant was sprayed immediately after the bacterial solution was coated on the medium,and the bactericidal rate was calculated at different time periods to investigate the bactericidal effect of the disinfectant on bacteria.Metal corrosion test tablets were immersed in disinfectants to assess the degree of erosion to clinical production equipment.The effect of light on bacteriostatic effect of disinfectant was also investigated.At the same time,clinical disinfection trials were carried out in a large-scale farm.The air and surface of the building were sampled and cultured before and after the use of the disinfectant,and the bactericidal effect of the disinfectant in clinical application was investigated by counting the total number of colonies,so as to comprehensively evaluate the disinfection effect and safety of the disinfectant.【Result】 In the laboratory disinfection evaluation, the results of prevention test showed that no bacteria colonized in the tested disinfectant group except Pseudomonas aeruginosa. The bactericidal test results showed that the test disinfectant could play a good sterilization effect within 10 min, the bactericidal rate of Staphylococcus aureus,Bacillus subtilis and Escherichia coli was over 99.80%.The bactericidal rate of Pseudomonas aeruginosa decreased after 6 h.The metal corrosion test results showed that the corrosion rate of 304 stainless steel under test for 72 h was 0.0047 mm/a,and there was basically no corrosion.The results of light test showed that there was no bacterial colonization in both the light avoiding and the non-light avoiding groups.In the clinical production investigation,compared with disinfectant control group,the total number of bacterial colonies in the air of hoggery in test disinfectant group was extremely significantly decreased (P<0.01),but the total number of bacterial colonies on the surface of objects had no significant difference (P＞0.05).【Conclusion】 The full spectrum TiO₂ disinfectant had a strong killing effect on common pathogens,and had a good performance in clinical efficacy evaluation.The bactericidal rate in the air was as high as 100%,and the use cost was low,which had a good application and development prospect,and provided a new scheme for the environmental disinfection of large-scale farms.

Key words: TiO₂; environmental disinfectant; sterilization; bacteriostasis

中图分类号:

S851.36

莫雪莲, 相亦飞, 李子杨, 钟雅文, 黄璐瑗, 赵林艺, 宁俊凯, 何家康. 新型环境消毒剂全光谱二氧化钛的消毒效果评价[J]. 中国畜牧兽医, 2025, 52(5): 2449-2457.

MO Xuelian, XIANG Yifei, LI Ziyang, ZHONG Yawen, HUANG Luyuan, ZHAO Linyi, NING Junkai, HE Jiakang. Evaluation of Disinfection Effect of New Environmental Disinfectant Full Spectrum TiO₂[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2449-2457.

参考文献

[1] 相亦飞,马箐阳,钟雅文,等.222 nm远紫外线的杀菌作用及安全性评价[J].中国畜牧兽医,2024,51(7):3109-3117.XIANG Y F,MA J Y,ZHONG Y W,et al.Bactericidal effect and safety evaluation of 222 nm far ultraviolet light[J].China Animal Husbandry & Veterinary Medicine,2024,51(7):3109-3117.(in Chinese)
[2] 胡云皓,刘燕,朱良全.我国畜禽细菌类主要兽医病原微生物及其疫苗研制与应用概况[J].微生物学通报,1-14[2024-09-10].https://doi.org/10.13344/j.microbiol.china.240480.HU Y H,LIU Y,ZHU L Q.Overview of bacterial pathogens and vaccines in livestock and poultry industry in China[J]. Microbiology China,1-14[2024-09-10].https://doi.org/10.13344/j.microbiol.china.240480.(in Chinese)
[3] KIM Y J,KIM J B,SONG C S,et al.Disinfection of various materials with 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride in hatchery facilities[J]. Animal Bioscience,2022,35(4):631-637.
[4] BAI Y,DING X,ZHAO Q,et al.Development of an organic acid compound disinfectant to control food-borne pathogens and its application in chicken slaughterhouses[J].Poultry Science,2022,101(6):101842.
[5] 王文钊,李妍,代飞,等.3种消毒剂对猪场4种常见细菌的杀菌效果试验[J].动物医学进展,2024,45(8):139-144.WANG W Z,LI Y,DAI F,et al.Bactericidal effect of three disinfectants on four common bacteria in pig farms[J].Progress in Veterinary Medicine,2024,45(8):139-144.(in Chinese)
[6] 张泽辉,吴运谱,王春田.屏障系统常用消毒剂对金黄色葡萄球菌的杀菌效果[J].动物医学进展,2023,44(7):107-111.ZHANG Z H,WU Y P,WANG C T.Bactericidal effect of disinfectants commonly used in barrier systems against Staphylococcus aureus[J]. Progress in Veterinary Medicine,2023,44(7):107-111.(in Chinese)
[7] 林旭东,马文瑾,徐向月,等.兽用消毒剂环境安全性研究进展[J].中国兽医杂志,2024,60(8):97-104.LIN X D,MA W J,XU X Y,et al.Research progress on the environmental safety of veterinary disinfectants[J].Chinese Journal of Veterinary Medicine,2024,60(8):97-104.(in Chinese)
[8] 杨爽,赵斯琴,杨慧英.铋基半导体复合二氧化钛光催化研究进展[J].无机盐工业,2022,54(6):38-45.YANG S,ZHAO S Q,YANG H Y.Research progress on photocatalysis by bismuth-based semiconductor composite TiO₂[J].Inorganic Chemicals Industry,2022,54(6):38-45.(in Chinese)
[9] 杨鹤云,李晓良,郑兴.TiO₂基异质结光催化剂应用于有机污染物降解的研究进展[J].化工新型材料,2023,51(6):64-69.YANG H Y,LI X L,ZHENG X.Research progress of TiO₂-based heterojunction photocatalyst for degradation of organic pollutants[J].New Chemical Materials,2023,51(6):64-69.(in Chinese)
[10] 南瑞春,赵玉恒,丁莎,等.TiO₂纳米材料抗微生物研究进展[J].河南化工,2024,41(9):32-35.NAN R C,ZHAO Y H,DING S,et al.Research progress on antimicrobial properties of TiO₂ nanomaterials[J].Henan Chemical Industry,2024,41(9):32-35.(in Chinese)
[11] 刘静,高正阳,王杰,等.共掺杂改性TiO₂光催化剂的研究进展[J].材料导报,2021,35(S1):42-47.LIU J,GAO Z Y,WANG J,et al.Research progress of co-doped TiO₂ photocatalyst[J].Materials Reports,2021,35(S1):42-47.(in Chinese)
[12] LINSEBIGLER A,LU G,YATES J.Photocatalysis on TiO₂ surfaces:Principles,mechanisms,and selected results[J].Chemical Reviews,1995,95:735-758.
[13] 王震,郑晓环,袁翼,等.光催化去除水中天然有机物的研究进展[J].工业水处理,2023,43(9):43-50.WANG Z,ZHENG X H,HUAN Y,et al.Progress of photocatalysis for the removal of natural organic matter from water[J].Industrial Water Treatment,2023,43(9):43-50.(in Chinese)
[14] 李朋朋,李传栋,陈真,等.癸甲溴铵溶液在鸡场不同应用场景的消毒效果研究[J].中国家禽,2024,46(9):128-134.LI P P,LI C D,CHEN Z,et al.Disinfection effect of deciquam solution in different application scenes of chicken farm[J].China Poultry,2024,46(9):128-134.(in Chinese)
[15] 中华人民共和国卫生部.消毒技术规范[S].北京:中华人民共和国卫生部,2002.MINISTRY OF HEALTH OF THE PEOPLE’S REPUBLIC OF CHINA.Technical standard for disinfection[S].Beijing:Ministry of Health of the People’s Republic of China,2002.(in Chinese)
[16] 国家疾病预防控制局.消毒剂金属腐蚀性评价方法:GB/T 38498—2020[S].北京:中国标准出版社,2020.NATIONAL ADMINISTRATION OF DISEASE PREVENTION AND CONTROL.Evaluation method for determining metal corrosion of disinfectant:GB/T 38498—2020[S].Beijing:Standards Press of China,2020.(in Chinese)
[17] 北京市农业局.动物养殖场消毒效果评价规范:DB11/T 1429—2017[S].北京:北京市质量技术监督局，2017.BEIJING MUNICIPAL AGRICULTURE BUREAU.Regulation of disinfectant effect evaluation of farms:DB11/T 1429—2017[S].Beijing:Beijing Municipal Bureau of Quality and Technical Supervision,2017.(in Chinese)
[18] 国家疾病预防控制局.公共场所卫生检验方法第3部分:空气微生物:GB/T 18204.3—2013[S].北京:中国标准出版社,2013.NATIONAL ADMINISTRATION OF DISEASE PREVENTION AND CONTROL.Examination methods for public places—Part 3:Airborne microorganism:GB/T 18204.3—2013[S].Beijing:Standards Press of China,2013.(in Chinese)
[19] 余叔文,王明霞,颜丽英.大气污染生物监测方法[M].广东:中山大学出版社,1993.YU S W,WANG M X,YAN L Y.Biological Monitoring Methods for Air Pollution[M].Guangdong:Sun Yat-sen University Press,1993.(in Chinese)
[20] 中华人民共和国农业部.畜禽场环境质量标准：NY/T388—1999[S].北京:中国标准出版社,1999.MINISTRY OF AGRICULTURE OF THE PEOPLE’S REPUBLIC OF CHINA.Environmental quality standard for the livestock and poultry farm:NY/T388—1999[S].Beijing:Standards Press of China,1999.(in Chinese)
[21] 彭海青,王阳,张志豪,等.规模鸡场湿帘循环系统添加微酸性电解水杀菌效果研究[J].中国家禽,2022,44(8):73-78.PENG H Q,WANG Y,ZHANG Z H,et al.Sterilization effect of adding slightly acidic electrolyzed water to wet curtain circulation systems in scale poultry farms[J].China Poultry,2022,44(8):73-78.(in Chinese)
[22] 张晓琴,王静,马仕洪.季铵盐类抑菌剂在药物制剂中的应用进展[J].中国抗生素杂志,2024,49(3):252-261.ZHANG X Q,WANG J,MA S H.Application progress of quaternary ammonium antimicrobial agents in pharmaceutical preparations[J].Chinese Journal of Antibiotics,2024,49(3):252-261.(in Chinese)
[23] 万冰洁,刘小雪,齐林光,等.TiO₂基光催化CO₂还原研究进展[J].应用化学,2024,41(5):637-658.WAN B J,LIU X X,QI L G,et al.Research progress of TiO₂-based photocatalytic CO₂ reduction[J].Chinese Journal of Applied Chemistry,2024,41(5):637-658.(in Chinese)
[24] 冯恋,王玥,李鹏辉,等.碳化细菌纤维素掺杂的氮化碳/二氧化钛光催化杀菌剂的制备及性能[J].环境化学,2023,42(11):3940-3950.FENG L,WANG Y,LI P H,et al.Preparation and properties of photocatalytic bactericide of carbonized bacterial cellulose doped carbon nitride/titanium dioxide[J].Environmental Chemistry,2023,42(11):3940-3950.(in Chinese)
[25] 李超群,石娟,周丽萍,等.Ag-HA/TiO₂抗菌剂的制备及在大黄鱼中的应用[J].食品与生物技术学报,2023,42(11):55-62.LI C Q,SHI J,ZHOU L P,et al.Preparation of Ag-HA/TiO₂ antibacterial agent and application in large yellow croaker[J].Journal of Food Science and Biotechnology,2023,42(11):55-62.(in Chinese)
[26] 常香玲,柴菲.氧化锌纳米复合抗菌材料的制备及性能研究[J].日用化学工业,2023,53(9):1051-1056.CHANG X L,CHAI F.Preparation and performance study of zinc oxide nanocomposite antibacterial materials[J].China Surfactant Detergent & Cosmetics,2023,53(9):1051-1056.(in Chinese)
[27] 石燕花,杜西领,曲湲,等.抗菌-超疏水性载银纳米二氧化钛-聚氨酯复合纤维素纸膜制备及油水乳化液分离的应用[J].东北林业大学学报,2022,50(1):116-122.SHI Y H,DU X L,QU Y,et al.Preparation of Ag@TiO₂ NPs/PU composite cellulose membrane and separating oil-water emulsion[J].Journal of Northeast Forestry University,2022,50(1):116-122.(in Chinese)
[28] 陈学山,张军华,李川川,等.纳米抗菌三聚氰胺浸渍胶膜纸饰面板的制备及性能研究[J].林产工业,2024,61(9):13-19.CHEN X S,ZHANG J H,LI C C,et al.Preparation and properties of antibacterial melamine impregnated paper decorative panels[J].China Forest Products Industry,2024,61(9):13-19.(in Chinese)
[29] 李万珅,姚红蕊,王娜.六方氮化硼@TiO₂/水性环氧涂层的制备及性能[J].精细化工,2025，42(2):306-312.LI W S,YAO H R,WANG N.Preparation and properties of h-BN@TiO₂/waterborne epoxy coating[J]. Fine Chemicals,2025，42(2):306-312.(in Chinese)
[30] 宋延霞,倪世恒,王峰,等.定安猪保种及改良现状调查[J].黑龙江畜牧兽医,2023，10:63-67.SONG Y X,NI S H,WANG F,et al.Investigation on breeding preservation and improvement of Ding’an pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2023，10:63-67.(in Chinese)
[31] 佟海山,陈遇英.我国兽用消毒剂的应用现状与对策[J].中国兽医杂志,2017,53(9):115-117.TONG H S,CHEN Y Y.Application status and countermeasures of veterinary disinfectants in China[J].Chinese Journal of Veterinary Medicine,2017,53(9):115-117.(in Chinese)
[32] 闫妍,高亚楠,李其玮,等.等离子体与纳米二氧化钛光触媒协同对室内空气消毒效果研究[J].中国消毒学杂志,2022,39(2):83-86.YAN Y,GAO Y N,LI Q W,et al.Purification and disinfection effect of indoor air by plasma coupling Ag/TiO₂ nanophotocatalysis system[J].Chinese Journal of Disinfection,2022,39(2):83-86.(in Chinese)