1株唾液乳杆菌的抑菌物质及益生作用

doi:10.16431/j.cnki.1671-7236.2023.09.033

摘要/Abstract

摘要： 【目的】研究1株唾液乳杆菌的抑菌物质和益生作用,探讨其在生产中应用的可能性。【方法】经真空冷冻唾液乳杆菌发酵液制得无细胞上清液(cell free supernatant,CFS),采用牛津杯法观察其体外抑菌效果,并通过改变pH、添加过氧化氢酶和多种蛋白酶的方法分析其抑菌物质;将20只SPF昆明小鼠随机分为对照组和唾液乳杆菌组2组,用于研究唾液乳杆菌的安全性和益生作用。【结果】CFS在体外对大肠杆菌和金黄色葡萄球菌有明显抑制作用;当CSF的pH为5.0时,抑菌活性消失,除胃蛋白酶外,CSF经过氧化氢酶和其他多种蛋白酶处理后,其抑菌活性与未处理的CSF相比均显著或极显著降低(P＜0.05;P＜0.01),表明抑菌物质含有有机酸、过氧化氢和蛋白类物质;唾液乳杆菌组小鼠成活率为100%,且十二指肠绒毛形态明显优于对照组,肝脏指数、脾脏指数和空肠Occludin基因mRNA的表达量均与对照组差异不显著(P＞0.05),而体重和空肠Claudin-2基因mRNA表达量极显著高于对照组(P＜0.01)。【结论】该唾液乳杆菌在体外有较强的抑菌能力且对小鼠益生作用良好,抑菌物质包含有机酸、过氧化氢和蛋白类物质,有作为动物微生态制剂开发的潜力。

关键词: 唾液乳杆菌; 抑菌物质; 脏器系数; Occludin; Caludin-2

Abstract: 【Objective】 The purpose of this experiment was to study the bacteriostatic substances and probiotics of Lactobacillus salivarius from chicken,and to explore the possibility of its application in production.【Method】 In this experiment,sell free supernatant (CFS) was prepared by vacuum freezing Lactobacillus salivarius fermentation broth.The antibacterial effect of CFS was observed by Oxford cup method,and the antibacterial substances were analyzed by changing pH,adding catalase and various proteases.Twenty mice were randomly divided into control group and Lactobacillus salivarius group to study the probiotic effects of Lactobacillus salivarius.【Result】 CFS had obvious inhibitory effect on Escherichia coli and Staphylococcus aureus in vitro.When the pH of CSF was 5.0,the antibacterial activity disappeared.Except pepsin,the antibacterial activity of CSF treated with catalase and other proteases decreased significantly compared with untreated CSF (P＜0.05 or P＜0.01),indicating that the antibacterial substances contained organic acids,hydrogen peroxide and protein substances.The survival rate of mice in Lactobacillus salivarius group was 100%,duodenal villus morphology was significantly better than that of control group,liver index,spleen index and jejunum Occludin mRNA expression had no signifcant difference with control group (P＞0.05).Body weight and expression of Claudin-2 mRNA in jejunum were significantly higher than those in control group (P＜0.01).【Conclusion】 Lactobacillus salivarius had strong bacteriostatic ability in vitro and had good probiotic effect on mice.The bacteriostatic substances included organic acids,hydrogen peroxide and protein substances,which had the potential to be used as animal microecological preparations.

Key words: Lactobacillus salivary; antimicrobial substances; organ index; Occludin; Claudin-2

中图分类号:

S831.5

李平, 李涛, 齐萌, 郭雪峰, 吴自豪, 车丽妍, 郭嘉栋, 陈荣. 1株唾液乳杆菌的抑菌物质及益生作用[J]. 中国畜牧兽医, 2023, 50(9): 3771-3780.

LI Ping, LI Tao, QI Meng, GUO Xuefeng, WU Zihao, CHE Liyan, GUO Jiadong, CHEN Rong. Bacteriostatic Substances and Probiotics of a Strain of Lactobacillus salivarius[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3771-3780.

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