响应面法优化珠芽蓼多糖提取工艺研究

doi:10.16431/j.cnki.1671-7236.2025.03.042

摘要/Abstract

摘要： 【目的】利用水提醇沉法提取珠芽蓼多糖,并通过响应面法优化提取工艺参数，进一步对其单糖组成进行初步分析。【方法】采用单因素试验考察提取温度、液料比、提取时间和超声功率对珠芽蓼多糖提取率的影响；以多糖提取率为响应值，运用Box-Behnken试验设计法设计4因素3水平试验并进行响应面优化，确定各影响因素与多糖提取率的关系；运用Sevag法对多糖进行脱蛋白纯化，采用气相色谱-质谱联用(GC-MS)法对珠芽蓼多糖的单糖组成进行检测。【结果】各影响因素对珠芽蓼多糖提取率的影响大小依次为提取温度＞超声功率＞提取时间＞液料比，获得的最佳提取工艺条件为：提取温度50 ℃、液料比8∶1、提取时间90 min、超声功率500 W，在该优化条件下所得的珠芽蓼多糖提取率为6.619%。GC-MS检测结果显示，珠芽蓼多糖主要由D-阿拉伯糖、D-甘露糖、葡萄糖和D-半乳糖组成，摩尔比为0.63∶0.57∶6.3∶2.5。【结论】利用响应面法对珠芽蓼多糖提取工艺进行了优化，初步检测了其单糖组成，研究结果为珠芽蓼多糖的工业化提取提供了技术支持。

关键词: 珠芽蓼; 多糖; 响应面法; 提取

Abstract: 【Objective】 The objective of this experiment was to extract Polygonum viviparum L.polysaccharide by water extraction and alcohol precipitation,and optimize the extraction parameters by response surface methodology,and further analyze the composition of monosaccharides.【Method】 The effects of extraction temperature,liquid-solid ratio,extraction time and ultrasonic power on the extraction yield of Polygonum viviparum L.polysaccharide were investigated by single factor test.With the extraction rate of polysaccharide as the response value,the Box-Behnken experimental design method was used to design the 4-factor and 3-level test and optimize the response surface to determine the relationship between the influencing factors and the extraction rate of polysaccharide.The polysaccharide was deproteinized and purified by Sevag method,and the monosaccharide composition of Polygonum viviparum L.polysaccharide was determined by gas chromatography-mass spectrometry (GC-MS).【Result】 The influencing factors on the extraction rate of Polygonum viviparum L.polysaccharide was extraction temperature ＞ ultrasonic power ＞ extraction time ＞ liquid-solid ratio.The optimal extraction conditions were as follows:Extraction temperature was 50 ℃,liquid-solid ratio was 8∶1,extraction time was 90 min and ultrasonic power was 500 W.Under the optimized conditions,the extraction rate of Polygonum viviparum L.polysaccharide was 6.619%.The results of GC-MS assay showed that the Polygonum viviparum L.polysaccharide was mainly composed of D-arabinose, D-mannose,glucose and D-galactose with a molar ratio of 0.63∶0.57∶6.3∶2.5.【Conclusion】 Response surface methodology was used to optimize the extraction process of Polygonum viviparum L.polysaccharide,and the monosaccharide composition was preliminarily determined.The results provided technical support for the industrial extraction of Polygonum viviparum L.polysaccharide.

Key words: Polygonum viviparum L.; polysaccharides; response surface methodology; extraction

中图分类号:

S853.72

满敬元, 刘宇, 邢小勇, 杨珍, 郝宝成, 拉巴次旦, 何俊峰, 吴金措姆. 响应面法优化珠芽蓼多糖提取工艺研究[J]. 中国畜牧兽医, 2025, 52(3): 1416-1427.

MAN Jingyuan, LIU Yu, XING Xiaoyong, YANG Zhen, HAO Baocheng, LABA Cidan, HE Junfeng, WUJIN Cuomu. Optimization of Extraction Process of Polysaccharides from Polygonum viviparum L.by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1416-1427.

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