单宁对南美白对虾生长性能、体成分、消化酶活性及抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2023.08.005

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (8): 3073-3083.doi: 10.16431/j.cnki.1671-7236.2023.08.005

• 营养与饲料 • 上一篇

单宁对南美白对虾生长性能、体成分、消化酶活性及抗氧化性能的影响

邱建强^1,2, 黄文^2,3, 赵红霞², 陈冰², 鲁慧杰², 刘振兴^3,4, 彭凯^2,3

1. 华中农业大学水产学院, 武汉 430070;
2. 广东省农业科学院动物科学研究所, 广东省畜禽育种与营养研究重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广州 510640;
3. 广东省农业科学院水产研究中心, 广州 510640;
4. 广东省农业科学院动物卫生研究所, 广州 510640

收稿日期:2023-01-06 发布日期:2023-07-27
通讯作者: 彭凯 E-mail:pengkai1016@126.com
作者简介:邱建强,E-mail:qiujianqiang1124@126.com。
基金资助:
广东省农业科学院协同创新中心课题(XT202301);国家自然科学基金(31902388);国家外国专家项目(G2022030077L);广东省自然科学基金面上项目(2018A0303130301、2021A1515010850);广州市基础与应用基础研究项目(202002030378);科技创新战略专项资金(高水平农科院建设-青年研究员项目)(R2021PY-QY001)

Effects of Hydrolysable and Condensed Tannins on Growth Performance, Body Composition,Digestive Enzyme Activities and Antioxidant Indexes of Litopenaeus vannamei

QIU Jianqiang^1,2, HUANG Wen^2,3, ZHAO Hongxia², CHEN Bing², LU Huijie², LIU Zhenxing^3,4, PENG Kai^2,3

1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China;
3. Aquatic Research Center of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
4. Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2023-01-06 Published:2023-07-27

摘要/Abstract

摘要： 【目的】研究水解单宁和缩合单宁对南美白对虾(Litopenaeus vannamei)生长性能、体成分、肠道消化酶活性、血清生化及抗氧化指标的影响。【方法】选取初始体重为1.04 g±0.02 g南美白对虾800尾,随机分为5组,每组4个重复,每个重复40尾虾。5个组分别为:CON(对照)、HT0.1(添加0.1%水解单宁)、HT0.2(添加0.2%水解单宁)、CT0.1(添加0.1%缩合单宁)、CT0.2(添加0.2%缩合单宁),养殖周期56 d。试验结束后统计对虾重量、数量、摄食量,计算生长性能;每缸随机选取一定数量对虾测定体成分,于围心腔采血分离血清,测定血清生化及抗氧化指标,解剖后分离肝胰腺测定肝胰腺抗氧化指标,分离肠道测定肠道消化酶活性。【结果】与CON组相比,①HT0.1组对虾终末体重(final body weight,FBW)、摄食量(feed intake,FI)、增重率(weight gain rate,WGR)和特定生长率(specific growth rate,SGR)显著提高(P＜0.05),CT0.1组对虾FI显著降低(P＜0.05),CT0.2组对虾FBW、FI、WGR和SGR均显著降低(P＜0.05);CT0.1和CT0.2组对虾肠道胰蛋白酶和脂肪酶活性均显著降低(P＜0.05)。②HT0.2、CT0.1、CT0.2组对虾血清谷丙转氨酶和谷草转氨酶活性显著升高(P＜0.05);HT0.1和HT0.2组对虾血清总抗氧化能力(total antioxidant capacity,T-AOC)显著提高(P＜0.05),HT0.1组对虾血清过氧化氢酶(catalase,CAT)活性显著提高(P＜0.05),CT0.2组对虾血清CAT活性显著降低(P＜0.05);HT0.1、HT0.2、CT0.1、CT0.2组对虾血清丙二醛(malondialdehyde,MDA)含量均显著降低(P＜0.05)。③CT0.1组对虾肝胰腺T-AOC显著提高(P＜0.05),HT0.1、HT0.2、CT0.1、CT0.2组对虾肝胰腺超氧化物歧化酶活性均显著提高(P＜0.05),HT0.1和CT0.1组对虾肝胰腺CAT活性均显著提高(P＜0.05),HT0.2组对虾肝胰腺MDA含量显著降低(P＜0.05)。【结论】综合评价南美白对虾的生长、消化和抗氧化指标,水解单宁的应用效果优于缩合单宁,饲料中添加水解单宁的适宜剂量为0.1%。

关键词: 单宁; 南美白对虾; 生长; 消化; 抗氧化

Abstract: 【Objective】 This study was conducted to evaluate the effects of hydrolysable and condensed tannins on growth performance,body composition,digestive enzyme activities,serum biochemistry and antioxidant indexes of Litopenaeus vannamei.【Method】 A total of 800 shrimp (initial body weight was 1.04 g±0.02 g) were randomly divided into 5 groups with 4 replicates per group and 40 shrimp per replicate.Five diets including CON (control),HT0.1 (adding 0.1% hydrolysable tannins),HT0.2 (adding 0.2% hydrolysable tannins),CT0.1 (adding 0.1% condensed tannins) and CT0.2 (adding 0.2% condensed tannins) were formulated.The experiment lasted for 56 d.At the end of the experiment,the weight,quantity,and feed intake of shrimp per tank were counted to calculate the growth performance.Shrimp were randomly selected from each tank to determine the body compositions.Blood was collected from the cardiocoelom of shrimp to separate serum for determination of serum biochemical and antioxidant indexes.Hepatopancreas of shrimp were separated to determine the antioxidant indicators.Intestine of shrimp was separated for determination of the intestinal digestive enzyme activity.【Result】 Compared with CON,the final body weight (FBW),feed intake (FI),weight gain rate (WGR) and specific growth rate (SGR) in HT0.1 were significantly increased (P＜0.05),the FI in CT0.1 was significantly decreased (P＜0.05),and the FBW,FI,WGR and SGR in CT0.2 were significantly decreased (P＜0.05).The intestinal trypsin and lipase activities of shrimp in CT0.1 and CT0.2 were significantly decreased (P＜0.05).Compared with CON,HT0.2,CT0.1 and CT0.2 had higher (P＜0.05) serum alanine transaminase and aspartate aminotransferase activities,HT0.1 and HT0.2 had higher (P＜0.05) total antioxidant capacity (T-AOC),HT0.1 had higher (P＜0.05) but CT0.2 had lower (P＜0.05) serum catalase (CAT) activities,the serum malondialdehyde (MDA) level of shrimp in HT0.1,HT0.2,CT0.1 and CT0.2 were decreased (P＜0.05).Compared with CON,the hepatic T-AOC of shrimp in CT0.1 was increased (P＜0.05),the hepatic superoxide dismutase activity of shrimp in HT0.1,HT0.2,CT0.1 and CT0.2 were increased (P＜0.05),the hepatic CAT activity of shrimp in HT0.1 and CT0.1 were increased (P＜0.05),and the hepatic MDA level of shrimp in HT0.2 was decreased (P＜0.05).【Conclusion】 Taking the growth,digestion and antioxidation of Litopenaeus vannamei as the comprehensive evaluation indexes,the effects of supplementation of hydrolysable tannins was better than that of condensed tannins,and the appropriate ratio of dietary hydrolysable tannins was 0.1%.

Key words: tannins; Litopenaeus vannamei; growth; digestion; antioxidation

中图分类号:

S816.7

邱建强, 黄文, 赵红霞, 陈冰, 鲁慧杰, 刘振兴, 彭凯. 单宁对南美白对虾生长性能、体成分、消化酶活性及抗氧化性能的影响[J]. 中国畜牧兽医, 2023, 50(8): 3073-3083.

QIU Jianqiang, HUANG Wen, ZHAO Hongxia, CHEN Bing, LU Huijie, LIU Zhenxing, PENG Kai. Effects of Hydrolysable and Condensed Tannins on Growth Performance, Body Composition,Digestive Enzyme Activities and Antioxidant Indexes of Litopenaeus vannamei[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3073-3083.

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单宁对南美白对虾生长性能、体成分、消化酶活性及抗氧化性能的影响

Effects of Hydrolysable and Condensed Tannins on Growth Performance, Body Composition,Digestive Enzyme Activities and Antioxidant Indexes of Litopenaeus vannamei

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