北京鸭和连城白鸭胸肌脂质组鉴定与分析

doi:10.16431/j.cnki.1671-7236.2023.03.006

摘要/Abstract

摘要： 【目的】研究鸭胸肌脂质谱,并探究北京鸭和连城白鸭胸肌脂质组差异。【方法】选取6周龄北京鸭和连城白鸭各28只并采集其胸肌组织,采用基于液相色谱-质谱联用方法(liquid chromatography-mass spectrometry,LC-MS)的脂质组学技术检测鸭胸肌中的脂质谱,通过多变量统计分析方法对北京鸭和连城白鸭胸肌脂质组的检测结果进行分析,采用差异倍数、t检验和偏最小二乘判别分析(PLS-DA)筛选差异脂质。【结果】鸭胸肌共被鉴定到950种脂质,涵盖6大类,即甘油磷脂类(GP)395种、甘油脂类(GL)365种、鞘脂类(SP)86种、脂肪酰类(FA)78种、甾醇脂类(ST)23种、异戊烯醇脂类(PR)3种;种类含量最多的脂质亚类为甘油三酯类(TG)283种。与北京鸭相比,连城白鸭中筛选到18种差异显著脂质:7个上调,11个下调;其中TG类最多且在北京鸭中含量高,同时这些TG富含饱和脂肪酸和单不饱和脂肪酸,而含超长链多不饱和脂肪酸(VLC-PUFA)的GP在连城白鸭中含量高。【结论】本研究获得了全面详细的鸭胸肌脂质谱,并筛选出了北京鸭和连城白鸭18种差异脂质标记物,为鸭的肌内脂质代谢、肉质研究及品质育种提供了科学的理论依据。

关键词: 北京鸭; 连城白鸭; 胸肌; 脂质组; 肌内脂质

Abstract: 【Objective】 The purpose of this experiment was to study the lipid profile of duck breast muscle and investigate the differences between the breast muscle lipidome of Pekin duck and Liancheng White duck.【Method】 28 Pekin ducks and 28 Liancheng White ducks were selected at 6-week-old and their breast muscle tissues were collected to detect the lipid profile used lipidomics techniques based on liquid chromatography-mass spectrometry (LC-MS).The results were analyzed by multivariate statistical analysis,and the differential lipids were screened by fold change,t-test and partial least squares discriminant analysis (PLS-DA).【Result】 A total of 950 lipids were identified in duck breast muscle,covering 6 categories,395 glycerophospholipids (GP),365 glycerolipids (GL),86 sphingolipids (SP),78 fatty acyls (FA),23 sterol lipids (ST) and 3 prenol lipids (PR);The most abundant lipid subgroup was triglycerides (TG) with 283 species.Compared with Pekin duck,18 lipids were screened in Liancheng White duck, 7 were upregulated and 11 were downregulated;Among them,TGs were the most abundant and high in Pekin duck,which were rich in saturated and monounsaturated fatty acids,while these GP containing very long chain polyunsaturated fatty acid (VLC-PUFA) were found to be high in Liancheng White duck.【Conclusion】 In this study,a comprehensive and detailed lipid profile of duck breast muscle was obtained,and 18 differential lipid markers were screened for Pekin duck and Liancheng White duck,this study provided a scientific and theoretical basis for intramuscular lipid metabolism,meat quality research and quality breeding of duck.

Key words: Pekin duck; Liancheng White duck; breast muscle; lipidome; intramuscular lipid

中图分类号:

S834

张鹤, 刘大鹏, 唐贺贺, 刘同, 余大新, 于思梦, 盖凯, 侯水生, 周正奎. 北京鸭和连城白鸭胸肌脂质组鉴定与分析[J]. 中国畜牧兽医, 2023, 50(3): 904-912.

ZHANG He, LIU Dapeng, TANG Hehe, LIU Tong, YU Daxin, YU Simeng, GAI Kai, HOU Shuisheng, ZHOU Zhengkui. Identification and Analysis of the Lipidome of the Breast Muscle of Pekin Duck and Liancheng White Duck[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 904-912.

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