镰刀菌毒素对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响

doi:10.16431/j.cnki.1671-7236.2015.09.026

摘要/Abstract

摘要： 试验旨在研究镰刀菌毒素污染日粮对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响。选择35日龄体重为(8.45±0.94) kg的健康三元杂交(杜×长×大)断奶雌性仔猪40头,随机分为2组,每组20个重复,每个重复1头猪。对照组饲喂基础日粮,试验组饲喂用霉变玉米和霉变玉米蛋白粉替代基础日粮中50%的玉米和玉米蛋白粉配制而成的镰刀菌毒素污染日粮(玉米赤霉烯酮(ZEN) 0.90 mg/kg,呕吐毒素(DON) 1.43 mg/kg,烟曲霉毒素(FUM) 5.85 mg/kg)。仔猪单栏饲喂,预试期7 d,正试期35 d。结果表明:①与对照组相比,试验组断奶仔猪血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(ALP)活性均显著升高(P<0.05),肝脏相对重显著降低(P<0.05);②与对照组相比,试验组断奶仔猪血清和肝脏中谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性均显著降低(P<0.05),而丙二醛(MDA)含量显著升高(P<0.05);③病理组织学观察发现,试验组断奶仔猪肝脏出现一定程度的组织损伤,部分肝小叶边缘发生明显的炎性细胞浸润,肝细胞发生局部空泡变性。由此可知,镰刀菌毒素污染日粮(0.90 mg/kg ZEN,1.43 mg/kg DON,5.85 mg/kg FUM)饲喂断奶仔猪可引起仔猪血清酶活性改变,肝脏抗氧化功能降低,肝脏组织出现一定程度的病理损伤,从而影响肝脏功能的发挥。

关键词: 镰刀菌毒素; 血清酶; 肝脏; 抗氧化功能; 组织病理学; 断奶仔猪

Abstract: The objective of the present study was to investigate the effects of Fusarium toxins contaminated diet on serum enzymes, liver antioxidant function and histopathology of weaned gilts.Forty healthy weaned gilts (Duroc×Landrace×Yorkshire) aged 35 d with body weight of (8.45±0.94) kg were randomly allocated into 2 groups with 20 replicates per group and 1 pigs per replicate.Gilts in the control group were fed with basal diet, and those in the experiment group were fed with Fusarium toxins contaminated diet made by replacing 50% of corn and corn gluten meal in the basal diet with the naturally contaminated corn and corn gluten meal (0.90 mg/kg ZEN, 1.43 mg/kg DON, 5.85 mg/kg FUM).Gilts were fed individually in a metabolic cage for 35 d after 7 d adaptation.The results showed as follows:① Compared with control group, the serum activities of AST, ALT and ALP in experimental group were significantly increased (P<0.05), and the liver index of experiment group was significantly decreased (P<0.05).②Compared with control group, the activities of GSH-Px and T-SOD in serum and liver of experimental group were significantly decreased (P<0.05), and the MDA content in serum and liver were significantly increased (P<0.05).③ Histological examination of liver in experiment group revealed a certain degree of damage, with obvious inflammatory cell infiltration partly at the margin of hepatic lobule and vacuoles degeneration of local hepatic cells.The results indicated that Fusarium toxins contaminated diets (0.90 mg/kg ZEN, 1.43 mg/kg DON, 5.85 mg/kg FUM) could change serum enzymes activity and impair liver antioxidant function, with a certain degree of pathological injury of liver in the present study, which had a negative impact on the normal liver function in weaned gilts.

Key words: Fusarium toxins; serum enzymes; liver; antioxidant function; histopathology; weaned gilts

中图分类号:

S828

陈宁波, 张崇玉, 姜淑贞, 黄丽波, 陈祥兴, 孙建立, 杨维仁. 镰刀菌毒素对断奶仔猪血清酶、肝脏抗氧化功能及组织病理学的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2384-2390.

CHEN Ning-bo, ZHANG Chong-yu, JIANG Shu-zhen, Huang Li-bo, CHEN Xiang-xing, SUN Jian-li, YANG Wei-ren. Effects of Fusarium Toxins on Serum Enzymes Activity,Liver Antioxidant Function and Histopathology of Weaned Gilts[J]. , 2015, 42(9): 2384-2390.

参考文献

[1] 甄阳光.我国主要饲料原料及产品中镰刀菌毒素污染及分布规律的研究[D].雅安:四川农业大学,2009.

[2] 敖志刚,陈代文.2006~2007年中国饲料及饲料原料霉菌毒素污染调查报告[J].中国畜牧兽医,2008,35(1):152-156.

[3] Placinta C M,D'Mello J P F,Macdonald A M C.A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins[J].Animal Feed Science and Technology,1999,78(1):21-37.

[4] Swamy H,Smith T K,Macdonald E J,et al.Effects of feeding a blend of grains naturally contaminated with mycotoxins on growth and immunological measurements of starter pigs,and the efficacy of a polymeric glucomannan mycotoxin adsorbent[J].Journal of Animal Science,2003,81(11):2792-2803.

[5] 苏军.镰刀菌毒素对猪的抗营养效应及其机制研究[D].雅安:四川农业大学,2008.

[6] Chen F,Ma Y,Xue C,et al.The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs[J].Journal of Veterinary Science,2008,9(1):39-44.

[7] 雷明彦.饲料中常见霉菌毒素对小鼠的联合毒性作用及机制的研究[D].武汉:华中农业大学,2013.

[8] Alm H,Gteising T,Brüssow K P,et al.The influence of the mycotoxins deoxynivalenol and zearalenol on in vitro maturation of pig oocytes and in vitro culture of pig zygotes[J].Toxicology in Vitro,2002,16(6):643-648.

[9] Dänicke S,Brüssow K P,Valenta H,et al.On the effects of graded levels of Fusarium toxin contaminated wheat in diets for gilts on feed intake,growth performance and metabolism of deoxynivalenol and zearalenone[J].Molecular Nutrition & Food Research,2005,49(10):932-943.

[10] Swamy H,Smith T K,Karrow N A,et al.Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on growth and immunological parameters of broiler chickens[J].Poultry Science,2004,83(4):533-543.

[11] Yegani M,Smith T K,Leeson S,et al.Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders[J].Poultry Science,2006,85(9):1541-1549.

[12] Burel C,Tanguy M,Guerre P,et al.Effect of low dose of fumonisins on pig health:Immune status,intestinal microbiota and sensitivity to Salmonella[J].Toxins,2013,5(4):841-864.

[13] 孟锐.镰刀菌毒素DON、ZEA及其联合染毒对小鼠肠道功能损伤的研究[D].雅安:四川农业大学,2012.

[14] NRC.National Research Council.Nutrient requirements of swine (11th ed)[M].Washington DC:National Academy Press,2012.

[15] European Commission.Commission recommendation of 17 August 2006 on the presence of deoxynivalenol,zearalenone,ochratoxin A,T-2 and HT-2 and fumonisins in products intended for animal feeding[R].Official Journal of European Union,2006.

[16] Homolka J.Clinic Biochemistry[M].Prague:Public House SZN,1969.

[17] Collins T F X,Sprando R L,Black T N,et al.Effects of zearalenone on in utero development in rats[J].Food and Chemical Toxicology,2006,44(9):1455-1465.

[18] 张弛,刘瑛,李华珠,等.血清谷丙转氨酶在正常范围内对非酒精性脂肪性肝病的预测[J].世界华人消化杂志,2011,19(8):841-844.

[19] Kaneko J J,Harvey J W,Bruss M L.Clinical biochemistry of domestic animals (5th Ed)[M].San Diego:Academic Press,1997.

[20] He C H,Fan Y H,Wang Y,et al.The individual and combined effects of deoxynivalenol and aflatoxin B₁ on primary hepatocytes of Cyprinus carpio [J].International Journal of Molecular Sciences (Online),2010,11(10):3760-3768.

[21] Tiemann U,Brüssow K P,Küchenmeister U,et al.Influence of diets with cereal grains contaminated by graded levels of two Fusarium toxins on selected enzymatic and histological parameters of liver in gilts[J].Food and Chemical Toxicology,2006,44(8):1228-1235.

[22] Huff W E,Kubena L F,Harvey R B,et al.Progression of aflatoxicosis in broiler chickens[J].Poultry Science,1986,65(10):1891-1899.

[23] Döll S,Dänicke S,Ueberschär K H,et al.Effects of graded levels of Fusarium toxin contaminated maize in diets for female weaned piglets[J].Archiv für Tierernahrung,2003,57(5):311-334.

[24] Bergsjø B,Langseth W,Nafstad I,et al.The effects of naturally deoxynivalenol-contaminated oats on the clinical condition,blood parameters,performance and carcass composition of growing pigs[J].Veterinary Research Communications,1993,17(4):283-294.

[25] Salah-Abbès J B,Abbès S,Ouanes Z,et al.Tunisian radish extract (Raphanus sativus) ameliorates the antioxidant status and mediates the oxidative stress of zeralenone in BALB/c mice[J].Journal of Applied Toxicology,2007,28(1):6-14.

[26] Jiang S Z,Yang Z B,Yang W R,et al.Effects of purified zearalenone on growth performance,organ size,serum metabolites,and oxidative stress in postweaning gilts[J].Journal of Animal Science,2011,89(10):3008-3015.

[27] 蒋竹英,李丽立,唐利华,等.DON污染饲粮添加竹炭和竹醋液对断奶仔猪抗氧化性能及小肠黏膜形态的影响[J].西北农林科技大学学报(自然科学版),2013,12:37-42.

[28] Sies H.Oxidative stress:From basic research to clinical application[J].The American Journal of Medicine,1991,3:31-38.

[29] 闫俊书,单安山,王环宇.五味子提取物对AA肉仔鸡抗氧化功能的影响[J].中国畜牧杂志,2008,44(17):33-37.

[30] Cheung C,Zheng G J,Lam P K S,et al.Relationship between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels,Perna viridis[J].Aquatic Toxicology,2001,52(3):189-203.

[31] Dröge W.Free radicals in the physiological control of cell function[J].Physiology Reviews,2002,82(1):47-95.

[32] Jiang S Z,Li Z,Wang G Y,et al.Effects of Fusarium mycotoxins with yeast cell wall absorbent on hematology,serum biochemistry and oxidative stress in broiler chickens[J].The Journal of Applied Poultry Research,2014,23(2):165-173.

[33] Kouadio J H,Mobio T A,Baudrimont I,et al.Comparative study of cytotoxicity and oxidative stress induced by deoxynivalenol,zearalenone or fumonisin B₁ in human intestinal cell line Caco-2[J].Toxicology,2005,213(1):56-65.

[34] Mclean M.The phytotoxicity of Fusarium metabolites:An update since 1989[J].Mycopathologia,1996,133(3):163-179.

[35] 何健.自然霉变玉米对肉鸭生产性能和免疫功能的影响及机制研究[D].雅安:四川农业大学,2011.

[36] 朱于斌.脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮及其联合染毒对小鼠肝肾损伤机制的研究[D].雅安:四川农业大学,2012.

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